TY - JOUR

T1 - Analysis of wild tomato introgression lines elucidates the genetic basis of transcriptome and metabolome variation underlying fruit traits and pathogen response

AU - Szymanski, Jedrzej

AU - Bocobza, Samuel

AU - Panda, Sayantan

AU - Sonawane, Prashant

AU - Cardenas, Pablo D.

AU - Lashbrooke, Justin

AU - Kamble, Avinash

AU - Shahaf, Nir

AU - Meir, Sagit

AU - Bovy, Arnaud

AU - Beekwilder, Jules

AU - Tikunov, Yury

AU - Romero de la Fuente, Irene

AU - Zamir, Dani

AU - Rogachev, Ilana

AU - Aharoni, Asaph

PY - 2020

Y1 - 2020

N2 - Wild tomato species represent a rich gene pool for numerous desirable traits lost during domestication. Here, we exploited an introgression population representing wild desert-adapted species and a domesticated cultivar to establish the genetic basis of gene expression and chemical variation accompanying the transfer of wild-species-associated fruit traits. Transcriptome and metabolome analysis of 580 lines coupled to pathogen sensitivity assays resulted in the identification of genomic loci associated with levels of hundreds of transcripts and metabolites. These associations occurred in hotspots representing coordinated perturbation of metabolic pathways and ripening-related processes. Here, we identify components of theSolanumalkaloid pathway, as well as genes and metabolites involved in pathogen defense and linking fungal resistance with changes in the fruit ripening regulatory network. Our results outline a framework for understanding metabolism and pathogen resistance during tomato fruit ripening and provide insights into key fruit quality traits.A study of 580 tomato lines resulting from a cross between a wild desert-adapted species and a domesticated cultivar elucidates the genetic basis of gene expression and metabolite variation associated with fruit traits and defense against pathogens.

AB - Wild tomato species represent a rich gene pool for numerous desirable traits lost during domestication. Here, we exploited an introgression population representing wild desert-adapted species and a domesticated cultivar to establish the genetic basis of gene expression and chemical variation accompanying the transfer of wild-species-associated fruit traits. Transcriptome and metabolome analysis of 580 lines coupled to pathogen sensitivity assays resulted in the identification of genomic loci associated with levels of hundreds of transcripts and metabolites. These associations occurred in hotspots representing coordinated perturbation of metabolic pathways and ripening-related processes. Here, we identify components of theSolanumalkaloid pathway, as well as genes and metabolites involved in pathogen defense and linking fungal resistance with changes in the fruit ripening regulatory network. Our results outline a framework for understanding metabolism and pathogen resistance during tomato fruit ripening and provide insights into key fruit quality traits.A study of 580 tomato lines resulting from a cross between a wild desert-adapted species and a domesticated cultivar elucidates the genetic basis of gene expression and metabolite variation associated with fruit traits and defense against pathogens.

KW - BOTRYTIS-CINEREA

KW - DISEASE RESISTANCE

KW - SPECTROMETRY DATA

KW - EXPRESSION

KW - BIOSYNTHESIS

KW - SUSCEPTIBILITY

KW - GENOME

KW - ARABIDOPSIS

KW - POPULATION

KW - PENNELLII

U2 - 10.1038/s41588-020-0690-6

DO - 10.1038/s41588-020-0690-6

M3 - Journal article

C2 - 32989321

VL - 52

SP - 1111

EP - 1121

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

ER -