Widespread biosynthesis of 16-carbon terpenoids in bacteria
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Widespread biosynthesis of 16-carbon terpenoids in bacteria. / Duan, Yao Tao; Koutsaviti, Aikaterini; Harizani, Maria; Ignea, Codruta; Roussis, Vassilios; Zhao, Yong; Ioannou, Efstathia; Kampranis, Sotirios C.
In: Nature Chemical Biology, Vol. 19, 2023, p. 1532-1539.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Widespread biosynthesis of 16-carbon terpenoids in bacteria
AU - Duan, Yao Tao
AU - Koutsaviti, Aikaterini
AU - Harizani, Maria
AU - Ignea, Codruta
AU - Roussis, Vassilios
AU - Zhao, Yong
AU - Ioannou, Efstathia
AU - Kampranis, Sotirios C.
N1 - Publisher Copyright: © 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2023
Y1 - 2023
N2 - Terpenoids are the most diverse group of specialized metabolites with numerous applications. Their biosynthesis is based on the five-carbon isoprene building block and, as a result, almost all terpenoids isolated to date are based on backbones that contain multiples of five carbon atoms. Intrigued by the discovery of an unusual bacterial terpenoid with a 16-carbon skeleton, here we investigate whether the biosynthesis of 16-carbon terpenoids is more widespread than this single example. We mine bacterial genomic information and identify potential C16 biosynthetic clusters in more than 700 sequenced genomes. We study selected clusters using a yeast synthetic biology platform and reveal that the encoded synthases produce at least 47 different noncanonical terpenoids. By thorough chemical analysis, we explain the structures of 13 C16 metabolites, most of which possess intricate highly strained bi- and tricyclic backbones. Our results unveil the existence of an extensive class of terpenoids in bacteria. [Figure not available: see fulltext.].
AB - Terpenoids are the most diverse group of specialized metabolites with numerous applications. Their biosynthesis is based on the five-carbon isoprene building block and, as a result, almost all terpenoids isolated to date are based on backbones that contain multiples of five carbon atoms. Intrigued by the discovery of an unusual bacterial terpenoid with a 16-carbon skeleton, here we investigate whether the biosynthesis of 16-carbon terpenoids is more widespread than this single example. We mine bacterial genomic information and identify potential C16 biosynthetic clusters in more than 700 sequenced genomes. We study selected clusters using a yeast synthetic biology platform and reveal that the encoded synthases produce at least 47 different noncanonical terpenoids. By thorough chemical analysis, we explain the structures of 13 C16 metabolites, most of which possess intricate highly strained bi- and tricyclic backbones. Our results unveil the existence of an extensive class of terpenoids in bacteria. [Figure not available: see fulltext.].
U2 - 10.1038/s41589-023-01445-9
DO - 10.1038/s41589-023-01445-9
M3 - Journal article
C2 - 37828399
AN - SCOPUS:85174015748
VL - 19
SP - 1532
EP - 1539
JO - Nature Chemical Biology
JF - Nature Chemical Biology
SN - 1552-4450
ER -
ID: 371374679