The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence

Research output: Contribution to journal › Journal article › Research › peer-review

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The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence. / Pentzold, Stefan; Zagrobelny, Mika; Roelsgaard, Pernille Sølvhøj; Møller, Birger Lindberg; Bak, Søren.

In: PLOS ONE, Vol. 9, No. 3, e91337, 2014.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pentzold, S, Zagrobelny, M, Roelsgaard, PS, Møller, BL & Bak, S 2014, 'The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence', PLOS ONE, vol. 9, no. 3, e91337. https://doi.org/10.1371/journal.pone.0091337

APA

Pentzold, S., Zagrobelny, M., Roelsgaard, P. S., Møller, B. L., & Bak, S. (2014). The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence. PLOS ONE, 9(3), [e91337]. https://doi.org/10.1371/journal.pone.0091337

Vancouver

Pentzold S, Zagrobelny M, Roelsgaard PS, Møller BL, Bak S. The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence. PLOS ONE. 2014;9(3). e91337. https://doi.org/10.1371/journal.pone.0091337

Author

Pentzold, Stefan ; Zagrobelny, Mika ; Roelsgaard, Pernille Sølvhøj ; Møller, Birger Lindberg ; Bak, Søren. / The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence. In: PLOS ONE. 2014 ; Vol. 9, No. 3.

Bibtex

@article{f5e7c7ba24264cfebac52b89ffeccb04,
title = "The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence",
abstract = "Cyanogenic glucosides (CNglcs) are widespread plant defence compounds that release toxic hydrogen cyanide by plant bglucosidaseactivity after tissue damage. Specialised insect herbivores have evolved counter strategies and some sequesterCNglcs, but the underlying mechanisms to keep CNglcs intact during feeding and digestion are unknown. We show thatCNglc-sequestering Zygaena filipendulae larvae combine behavioural, morphological, physiological and biochemicalstrategies at different time points during feeding and digestion to avoid toxic hydrolysis of the CNglcs present in their Lotusfood plant, i.e. cyanogenesis. We found that a high feeding rate limits the time for plant b-glucosidases to hydrolyse CNglcs.Larvae performed leaf-snipping, a minimal disruptive feeding mode that prevents mixing of plant b-glucosidases andCNglcs. Saliva extracts did not inhibit plant cyanogenesis. However, a highly alkaline midgut lumen inhibited the activity ofingested plant b-glucosidases significantly. Moreover, insect b-glucosidases from the saliva and gut tissue did not hydrolysethe CNglcs present in Lotus. The strategies disclosed may also be used by other insect species to overcome CNglc-basedplant defence and to sequester these compounds intact.",
author = "Stefan Pentzold and Mika Zagrobelny and Roelsgaard, {Pernille S{\o}lvh{\o}j} and M{\o}ller, {Birger Lindberg} and S{\o}ren Bak",
note = "OA",
year = "2014",
doi = "10.1371/journal.pone.0091337",
language = "English",
volume = "9",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence

AU - Pentzold, Stefan

AU - Zagrobelny, Mika

AU - Roelsgaard, Pernille Sølvhøj

AU - Møller, Birger Lindberg

AU - Bak, Søren

N1 - OA

PY - 2014

Y1 - 2014

N2 - Cyanogenic glucosides (CNglcs) are widespread plant defence compounds that release toxic hydrogen cyanide by plant bglucosidaseactivity after tissue damage. Specialised insect herbivores have evolved counter strategies and some sequesterCNglcs, but the underlying mechanisms to keep CNglcs intact during feeding and digestion are unknown. We show thatCNglc-sequestering Zygaena filipendulae larvae combine behavioural, morphological, physiological and biochemicalstrategies at different time points during feeding and digestion to avoid toxic hydrolysis of the CNglcs present in their Lotusfood plant, i.e. cyanogenesis. We found that a high feeding rate limits the time for plant b-glucosidases to hydrolyse CNglcs.Larvae performed leaf-snipping, a minimal disruptive feeding mode that prevents mixing of plant b-glucosidases andCNglcs. Saliva extracts did not inhibit plant cyanogenesis. However, a highly alkaline midgut lumen inhibited the activity ofingested plant b-glucosidases significantly. Moreover, insect b-glucosidases from the saliva and gut tissue did not hydrolysethe CNglcs present in Lotus. The strategies disclosed may also be used by other insect species to overcome CNglc-basedplant defence and to sequester these compounds intact.

AB - Cyanogenic glucosides (CNglcs) are widespread plant defence compounds that release toxic hydrogen cyanide by plant bglucosidaseactivity after tissue damage. Specialised insect herbivores have evolved counter strategies and some sequesterCNglcs, but the underlying mechanisms to keep CNglcs intact during feeding and digestion are unknown. We show thatCNglc-sequestering Zygaena filipendulae larvae combine behavioural, morphological, physiological and biochemicalstrategies at different time points during feeding and digestion to avoid toxic hydrolysis of the CNglcs present in their Lotusfood plant, i.e. cyanogenesis. We found that a high feeding rate limits the time for plant b-glucosidases to hydrolyse CNglcs.Larvae performed leaf-snipping, a minimal disruptive feeding mode that prevents mixing of plant b-glucosidases andCNglcs. Saliva extracts did not inhibit plant cyanogenesis. However, a highly alkaline midgut lumen inhibited the activity ofingested plant b-glucosidases significantly. Moreover, insect b-glucosidases from the saliva and gut tissue did not hydrolysethe CNglcs present in Lotus. The strategies disclosed may also be used by other insect species to overcome CNglc-basedplant defence and to sequester these compounds intact.

U2 - 10.1371/journal.pone.0091337

DO - 10.1371/journal.pone.0091337

M3 - Journal article

C2 - 24625698

VL - 9

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 3

M1 - e91337

ER -

ID: 104719216