The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells

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The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells. / Zacharopoulou, Nefeli; Tsapara, Anna; Kallergi, Galatea; Schmid, Evi; Alkahtani, Saad; Alarifi, Saud; Tsichlis, Philip N; Kampranis, S.C.; Stournaras, Christos.

In: Cellular Physiology and Biochemistry, Vol. 47, No. 1, 2018, p. 368-377.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zacharopoulou, N, Tsapara, A, Kallergi, G, Schmid, E, Alkahtani, S, Alarifi, S, Tsichlis, PN, Kampranis, SC & Stournaras, C 2018, 'The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells', Cellular Physiology and Biochemistry, vol. 47, no. 1, pp. 368-377. https://doi.org/10.1159/000489917

APA

Zacharopoulou, N., Tsapara, A., Kallergi, G., Schmid, E., Alkahtani, S., Alarifi, S., Tsichlis, P. N., Kampranis, S. C., & Stournaras, C. (2018). The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells. Cellular Physiology and Biochemistry, 47(1), 368-377. https://doi.org/10.1159/000489917

Vancouver

Zacharopoulou N, Tsapara A, Kallergi G, Schmid E, Alkahtani S, Alarifi S et al. The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells. Cellular Physiology and Biochemistry. 2018;47(1):368-377. https://doi.org/10.1159/000489917

Author

Zacharopoulou, Nefeli ; Tsapara, Anna ; Kallergi, Galatea ; Schmid, Evi ; Alkahtani, Saad ; Alarifi, Saud ; Tsichlis, Philip N ; Kampranis, S.C. ; Stournaras, Christos. / The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells. In: Cellular Physiology and Biochemistry. 2018 ; Vol. 47, No. 1. pp. 368-377.

Bibtex

@article{cee5ebb8890247aa96971d37e24798bd,
title = "The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells",
abstract = "BACKGROUND/AIMS: The epigenetic factor KDM2B is a histone demethylase expressed in various tumors. Recently, we have shown that KDM2B regulates actin cytoskeleton organization, small Rho GTPases signaling, cell-cell adhesion and migration of prostate tumor cells. In the present study, we addressed its role in regulating EMT and small GTPases expression in colon tumor cells.METHODS: We used RT-PCR for the transcriptional analysis of various genes, Western blotting for the assessment of protein expression and immunofluorescence microscopy for visualization of fluorescently labeled proteins.RESULTS: We report here that KDM2B regulates EZH2 and BMI1 in HCT116 colon tumor cells. Knockdown of this epigenetic factor induced potent up-regulation of the protein levels of the epithelial markers E-cadherin and ZO-1, while the mesenchymal marker N-cadherin was downregulated. On the other hand, KDM2B overexpression downregulated the levels of both epithelial markers and upregulated the mesenchymal marker, suggesting control of EMT by KDM2B. In addition, RhoA, RhoB and RhoC protein levels diminished upon KDM2B-knockdown, while all three small GTPases became upregulated in KDM2B-overexpressing HCT116 cell clones. Interestingly, Rac1 GTPase level increased upon KDM2B-knockdown and diminished in KDM2B-overexpressing HCT116 colon tumor- and DU-145 prostate cancer cells.CONCLUSIONS: These results establish a clear functional role of the epigenetic factor KDM2B in the regulation of EMT and small-GTPases expression in colon tumor cells and further support the recently postulated oncogenic role of this histone demethylase in various tumors.",
keywords = "Colonic Neoplasms/genetics, Enhancer of Zeste Homolog 2 Protein/genetics, Epigenesis, Genetic, Epithelial-Mesenchymal Transition, F-Box Proteins/genetics, Gene Expression Regulation, Neoplastic, HCT116 Cells, Humans, Jumonji Domain-Containing Histone Demethylases/genetics, Monomeric GTP-Binding Proteins/genetics",
author = "Nefeli Zacharopoulou and Anna Tsapara and Galatea Kallergi and Evi Schmid and Saad Alkahtani and Saud Alarifi and Tsichlis, {Philip N} and S.C. Kampranis and Christos Stournaras",
year = "2018",
doi = "10.1159/000489917",
language = "English",
volume = "47",
pages = "368--377",
journal = "Cellular Physiology and Biochemistry",
issn = "1015-8987",
publisher = "S Karger AG",
number = "1",

}

RIS

TY - JOUR

T1 - The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells

AU - Zacharopoulou, Nefeli

AU - Tsapara, Anna

AU - Kallergi, Galatea

AU - Schmid, Evi

AU - Alkahtani, Saad

AU - Alarifi, Saud

AU - Tsichlis, Philip N

AU - Kampranis, S.C.

AU - Stournaras, Christos

PY - 2018

Y1 - 2018

N2 - BACKGROUND/AIMS: The epigenetic factor KDM2B is a histone demethylase expressed in various tumors. Recently, we have shown that KDM2B regulates actin cytoskeleton organization, small Rho GTPases signaling, cell-cell adhesion and migration of prostate tumor cells. In the present study, we addressed its role in regulating EMT and small GTPases expression in colon tumor cells.METHODS: We used RT-PCR for the transcriptional analysis of various genes, Western blotting for the assessment of protein expression and immunofluorescence microscopy for visualization of fluorescently labeled proteins.RESULTS: We report here that KDM2B regulates EZH2 and BMI1 in HCT116 colon tumor cells. Knockdown of this epigenetic factor induced potent up-regulation of the protein levels of the epithelial markers E-cadherin and ZO-1, while the mesenchymal marker N-cadherin was downregulated. On the other hand, KDM2B overexpression downregulated the levels of both epithelial markers and upregulated the mesenchymal marker, suggesting control of EMT by KDM2B. In addition, RhoA, RhoB and RhoC protein levels diminished upon KDM2B-knockdown, while all three small GTPases became upregulated in KDM2B-overexpressing HCT116 cell clones. Interestingly, Rac1 GTPase level increased upon KDM2B-knockdown and diminished in KDM2B-overexpressing HCT116 colon tumor- and DU-145 prostate cancer cells.CONCLUSIONS: These results establish a clear functional role of the epigenetic factor KDM2B in the regulation of EMT and small-GTPases expression in colon tumor cells and further support the recently postulated oncogenic role of this histone demethylase in various tumors.

AB - BACKGROUND/AIMS: The epigenetic factor KDM2B is a histone demethylase expressed in various tumors. Recently, we have shown that KDM2B regulates actin cytoskeleton organization, small Rho GTPases signaling, cell-cell adhesion and migration of prostate tumor cells. In the present study, we addressed its role in regulating EMT and small GTPases expression in colon tumor cells.METHODS: We used RT-PCR for the transcriptional analysis of various genes, Western blotting for the assessment of protein expression and immunofluorescence microscopy for visualization of fluorescently labeled proteins.RESULTS: We report here that KDM2B regulates EZH2 and BMI1 in HCT116 colon tumor cells. Knockdown of this epigenetic factor induced potent up-regulation of the protein levels of the epithelial markers E-cadherin and ZO-1, while the mesenchymal marker N-cadherin was downregulated. On the other hand, KDM2B overexpression downregulated the levels of both epithelial markers and upregulated the mesenchymal marker, suggesting control of EMT by KDM2B. In addition, RhoA, RhoB and RhoC protein levels diminished upon KDM2B-knockdown, while all three small GTPases became upregulated in KDM2B-overexpressing HCT116 cell clones. Interestingly, Rac1 GTPase level increased upon KDM2B-knockdown and diminished in KDM2B-overexpressing HCT116 colon tumor- and DU-145 prostate cancer cells.CONCLUSIONS: These results establish a clear functional role of the epigenetic factor KDM2B in the regulation of EMT and small-GTPases expression in colon tumor cells and further support the recently postulated oncogenic role of this histone demethylase in various tumors.

KW - Colonic Neoplasms/genetics

KW - Enhancer of Zeste Homolog 2 Protein/genetics

KW - Epigenesis, Genetic

KW - Epithelial-Mesenchymal Transition

KW - F-Box Proteins/genetics

KW - Gene Expression Regulation, Neoplastic

KW - HCT116 Cells

KW - Humans

KW - Jumonji Domain-Containing Histone Demethylases/genetics

KW - Monomeric GTP-Binding Proteins/genetics

U2 - 10.1159/000489917

DO - 10.1159/000489917

M3 - Journal article

C2 - 29772566

VL - 47

SP - 368

EP - 377

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

SN - 1015-8987

IS - 1

ER -

ID: 209366716