Recombinant human tumour necrosis factor increases cytosolic free calcium in murine fibroblasts and stimulates inositol phosphate formation in L-M and arachidonic acid release in 3T3 cells
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Recombinant human tumour necrosis factor increases cytosolic free calcium in murine fibroblasts and stimulates inositol phosphate formation in L-M and arachidonic acid release in 3T3 cells. / Bouchelouche, P N; Bendtzen, K; Bak, S; Nielsen, O H.
In: Cellular Signalling, Vol. 2, No. 5, 1990, p. 479-87.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Recombinant human tumour necrosis factor increases cytosolic free calcium in murine fibroblasts and stimulates inositol phosphate formation in L-M and arachidonic acid release in 3T3 cells
AU - Bouchelouche, P N
AU - Bendtzen, K
AU - Bak, S
AU - Nielsen, O H
PY - 1990
Y1 - 1990
N2 - Stimulation of murine L-M and 3T3 fibroblasts with human recombinant tumour necrosis factor (rTNF) resulted in an increase in the cytosolic free Ca2+ concentration ([Ca2+]i). In 3T3 cells rTNF also induced release and metabolization of arachidonic acid, whereas in L-M cells rTNF provoked rapid increases in the levels of inositol mono-, bis- and trisphosphates (IP1, IP2 and IP3). In these cells the Ca2+ response was also observed in Ca2+ free medium, suggesting that rTNF promotes mobilization of Ca2+ from intracellular stores. In 3T3 cells, however, Ca2+ originated from the extracellular space, since the response was abolished in medium containing 1 mM EGTA. Both rTNF-induced calcium responses were inhibited by a specific rabbit IgG antibody to rTNF but not by 1-verapamil, a blocker potential-operated calcium channels. These results suggest that increased formation of inositol phosphates, arachidonic acid release and increased cytosolic free Ca2+ are involved in the biological effects of rTNF. However, rTNF generate these signals by different mechanisms depending upon the target cell.
AB - Stimulation of murine L-M and 3T3 fibroblasts with human recombinant tumour necrosis factor (rTNF) resulted in an increase in the cytosolic free Ca2+ concentration ([Ca2+]i). In 3T3 cells rTNF also induced release and metabolization of arachidonic acid, whereas in L-M cells rTNF provoked rapid increases in the levels of inositol mono-, bis- and trisphosphates (IP1, IP2 and IP3). In these cells the Ca2+ response was also observed in Ca2+ free medium, suggesting that rTNF promotes mobilization of Ca2+ from intracellular stores. In 3T3 cells, however, Ca2+ originated from the extracellular space, since the response was abolished in medium containing 1 mM EGTA. Both rTNF-induced calcium responses were inhibited by a specific rabbit IgG antibody to rTNF but not by 1-verapamil, a blocker potential-operated calcium channels. These results suggest that increased formation of inositol phosphates, arachidonic acid release and increased cytosolic free Ca2+ are involved in the biological effects of rTNF. However, rTNF generate these signals by different mechanisms depending upon the target cell.
KW - Animals
KW - Antibodies
KW - Arachidonic Acids/metabolism
KW - Calcium/metabolism
KW - Cell Line
KW - Cytosol/metabolism
KW - Endotoxins/pharmacology
KW - Hot Temperature
KW - Humans
KW - Inositol Phosphates/metabolism
KW - Kinetics
KW - Mice
KW - Recombinant Proteins/pharmacology
KW - Spectrometry, Fluorescence
KW - Tumor Necrosis Factor-alpha/immunology
M3 - Journal article
C2 - 2278808
VL - 2
SP - 479
EP - 487
JO - Cellular Signalling
JF - Cellular Signalling
SN - 0898-6568
IS - 5
ER -
ID: 218728320