Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

Research output: Contribution to journal › Journal article › Research › peer-review

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Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes. / Trikka, Fotini A.; Nikolaidis, Alexandros; Ignea, Codruta; Tsaballa, Aphrodite; Tziveleka, Leto-Aikaterini; Ioannou, Efstathia; Roussis, Vassilios; Stea, Eleni A.; Božić, Dragana; Argiriou, Anagnostis; Kanellis, Angelos K.; Kampranis, Sotirios C.; Makris, Antonios M.

In: BMC Genomics, Vol. 16, 935, 2015.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Trikka, FA, Nikolaidis, A, Ignea, C, Tsaballa, A, Tziveleka, L-A, Ioannou, E, Roussis, V, Stea, EA, Božić, D, Argiriou, A, Kanellis, AK, Kampranis, SC & Makris, AM 2015, 'Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes', BMC Genomics, vol. 16, 935. https://doi.org/10.1186/s12864-015-2147-3

APA

Trikka, F. A., Nikolaidis, A., Ignea, C., Tsaballa, A., Tziveleka, L-A., Ioannou, E., Roussis, V., Stea, E. A., Božić, D., Argiriou, A., Kanellis, A. K., Kampranis, S. C., & Makris, A. M. (2015). Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes. BMC Genomics, 16, [935]. https://doi.org/10.1186/s12864-015-2147-3

Vancouver

Trikka FA, Nikolaidis A, Ignea C, Tsaballa A, Tziveleka L-A, Ioannou E et al. Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes. BMC Genomics. 2015;16. 935. https://doi.org/10.1186/s12864-015-2147-3

Author

Trikka, Fotini A. ; Nikolaidis, Alexandros ; Ignea, Codruta ; Tsaballa, Aphrodite ; Tziveleka, Leto-Aikaterini ; Ioannou, Efstathia ; Roussis, Vassilios ; Stea, Eleni A. ; Božić, Dragana ; Argiriou, Anagnostis ; Kanellis, Angelos K. ; Kampranis, Sotirios C. ; Makris, Antonios M. / Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes. In: BMC Genomics. 2015 ; Vol. 16.

Bibtex

@article{55e6c9ca15b549d7aa8d8627e33bb85a,
title = "Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes",
abstract = "BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.",
author = "Trikka, {Fotini A.} and Alexandros Nikolaidis and Codruta Ignea and Aphrodite Tsaballa and Leto-Aikaterini Tziveleka and Efstathia Ioannou and Vassilios Roussis and Stea, {Eleni A.} and Dragana Bo{\v z}i{\'c} and Anagnostis Argiriou and Kanellis, {Angelos K.} and Kampranis, {Sotirios C.} and Makris, {Antonios M.}",
year = "2015",
doi = "10.1186/s12864-015-2147-3",
language = "English",
volume = "16",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

AU - Trikka, Fotini A.

AU - Nikolaidis, Alexandros

AU - Ignea, Codruta

AU - Tsaballa, Aphrodite

AU - Tziveleka, Leto-Aikaterini

AU - Ioannou, Efstathia

AU - Roussis, Vassilios

AU - Stea, Eleni A.

AU - Božić, Dragana

AU - Argiriou, Anagnostis

AU - Kanellis, Angelos K.

AU - Kampranis, Sotirios C.

AU - Makris, Antonios M.

PY - 2015

Y1 - 2015

N2 - BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.

AB - BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.

U2 - 10.1186/s12864-015-2147-3

DO - 10.1186/s12864-015-2147-3

M3 - Journal article

C2 - 26572682

VL - 16

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 935

ER -

ID: 159084476