Collagen-Containing Fish Sidestream-Derived Protein Hydrolysates Support Skin Repair via Chemokine Induction
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Collagen-Containing Fish Sidestream-Derived Protein Hydrolysates Support Skin Repair via Chemokine Induction. / Lapi, Ioanna; Kolliniati, Ourania; Aspevik, Tone; Deiktakis, Eleftherios E.; Axarlis, Konstantinos; Daskalaki, Maria G.; Dermitzaki, Eirini; Tzardi, Maria; Kampranis, Sotirios C.; El Marsni, Zouhir; Kousoulaki, Katerina C.; Tsatsanis, Christos; Venihaki, Maria.
In: Marine Drugs, Vol. 19, No. 7, 396, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Collagen-Containing Fish Sidestream-Derived Protein Hydrolysates Support Skin Repair via Chemokine Induction
AU - Lapi, Ioanna
AU - Kolliniati, Ourania
AU - Aspevik, Tone
AU - Deiktakis, Eleftherios E.
AU - Axarlis, Konstantinos
AU - Daskalaki, Maria G.
AU - Dermitzaki, Eirini
AU - Tzardi, Maria
AU - Kampranis, Sotirios C.
AU - El Marsni, Zouhir
AU - Kousoulaki, Katerina C.
AU - Tsatsanis, Christos
AU - Venihaki, Maria
PY - 2021
Y1 - 2021
N2 - Restoring homeostasis following tissue damage requires a dynamic and tightly orchestrated sequence of molecular and cellular events that ensure repair and healing. It is well established that nutrition directly affects skin homeostasis, while malnutrition causes impaired tissue healing. In this study, we utilized fish sidestream-derived protein hydrolysates including fish collagen as dietary supplements, and investigated their effect on the skin repair process using a murine model of cutaneous wound healing. We explored potential differences in wound closure and histological morphology between diet groups, and analyzed the expression and production of factors that participate in different stages of the repair process. Dietary supplementation with fish sidestream-derived collagen alone (Collagen), or in combination with a protein hydrolysate derived from salmon heads (HSH), resulted in accelerated healing. Chemical analysis of the tested extracts revealed that Collagen had the highest protein content and that HSH contained the great amount of zinc, known to support immune responses. Indeed, tissues from mice fed with collagen-containing supplements exhibited an increase in the expression levels of chemokines, important for the recruitment of immune cells into the damaged wound region. Moreover, expression of a potent angiogenic factor, vascular endothelial growth factor-A (VEGF-A), was elevated followed by enhanced collagen deposition. Our findings suggest that a 5%-supplemented diet with marine collagen-enriched supplements promotes tissue repair in the model of cutaneous wound healing, proposing a novel health-promoting use of fish sidestreams.
AB - Restoring homeostasis following tissue damage requires a dynamic and tightly orchestrated sequence of molecular and cellular events that ensure repair and healing. It is well established that nutrition directly affects skin homeostasis, while malnutrition causes impaired tissue healing. In this study, we utilized fish sidestream-derived protein hydrolysates including fish collagen as dietary supplements, and investigated their effect on the skin repair process using a murine model of cutaneous wound healing. We explored potential differences in wound closure and histological morphology between diet groups, and analyzed the expression and production of factors that participate in different stages of the repair process. Dietary supplementation with fish sidestream-derived collagen alone (Collagen), or in combination with a protein hydrolysate derived from salmon heads (HSH), resulted in accelerated healing. Chemical analysis of the tested extracts revealed that Collagen had the highest protein content and that HSH contained the great amount of zinc, known to support immune responses. Indeed, tissues from mice fed with collagen-containing supplements exhibited an increase in the expression levels of chemokines, important for the recruitment of immune cells into the damaged wound region. Moreover, expression of a potent angiogenic factor, vascular endothelial growth factor-A (VEGF-A), was elevated followed by enhanced collagen deposition. Our findings suggest that a 5%-supplemented diet with marine collagen-enriched supplements promotes tissue repair in the model of cutaneous wound healing, proposing a novel health-promoting use of fish sidestreams.
KW - collagen
KW - fish sidestreams
KW - chemokines
KW - nutrition
KW - CHRONIC WOUNDS
KW - KERATINOCYTE DIFFERENTIATION
KW - MOLECULAR-BIOLOGY
KW - PROLIFERATION
KW - MACROPHAGE
KW - PATHOPHYSIOLOGY
KW - ANGIOGENESIS
KW - ACTIVATION
KW - NUTRITION
KW - MIGRATION
U2 - 10.3390/md19070396
DO - 10.3390/md19070396
M3 - Journal article
C2 - 34356821
VL - 19
JO - Marine Drugs
JF - Marine Drugs
SN - 1660-3397
IS - 7
M1 - 396
ER -
ID: 275380766