Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. / Laursen, Tomas; Borch-Jensen, Jonas; Baden, Camilla Knudsen; Bavishi, Krutika; Torta, Federico; Martens, Helle Juel; Silvestro, Daniele; Hatzakis, Nikos; Wenk, Markus R; Dafforn, Timothy R; Olsen, Carl Erik; Motawie, Mohammed Saddik; Hamberger, Björn Robert; Møller, Birger Lindberg; Bassard, Jean-Étienne André.

In: Science (New York, N.Y.), Vol. 354, No. 6314, 2016, p. 890-893.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Laursen, T, Borch-Jensen, J, Baden, CK, Bavishi, K, Torta, F, Martens, HJ, Silvestro, D, Hatzakis, N, Wenk, MR, Dafforn, TR, Olsen, CE, Motawie, MS, Hamberger, BR, Møller, BL & Bassard, J-ÉA 2016, 'Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum', Science (New York, N.Y.), vol. 354, no. 6314, pp. 890-893. https://doi.org/10.1126/science.aag2347

APA

Laursen, T., Borch-Jensen, J., Baden, C. K., Bavishi, K., Torta, F., Martens, H. J., Silvestro, D., Hatzakis, N., Wenk, M. R., Dafforn, T. R., Olsen, C. E., Motawie, M. S., Hamberger, B. R., Møller, B. L., & Bassard, J-É. A. (2016). Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. Science (New York, N.Y.), 354(6314), 890-893. https://doi.org/10.1126/science.aag2347

Vancouver

Laursen T, Borch-Jensen J, Baden CK, Bavishi K, Torta F, Martens HJ et al. Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. Science (New York, N.Y.). 2016;354(6314):890-893. https://doi.org/10.1126/science.aag2347

Author

Laursen, Tomas ; Borch-Jensen, Jonas ; Baden, Camilla Knudsen ; Bavishi, Krutika ; Torta, Federico ; Martens, Helle Juel ; Silvestro, Daniele ; Hatzakis, Nikos ; Wenk, Markus R ; Dafforn, Timothy R ; Olsen, Carl Erik ; Motawie, Mohammed Saddik ; Hamberger, Björn Robert ; Møller, Birger Lindberg ; Bassard, Jean-Étienne André. / Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. In: Science (New York, N.Y.). 2016 ; Vol. 354, No. 6314. pp. 890-893.

Bibtex

@article{20be4fac793c4d2983b0af0b409234ff,
title = "Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum",
abstract = "Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.",
author = "Tomas Laursen and Jonas Borch-Jensen and Baden, {Camilla Knudsen} and Krutika Bavishi and Federico Torta and Martens, {Helle Juel} and Daniele Silvestro and Nikos Hatzakis and Wenk, {Markus R} and Dafforn, {Timothy R} and Olsen, {Carl Erik} and Motawie, {Mohammed Saddik} and Hamberger, {Bj{\"o}rn Robert} and M{\o}ller, {Birger Lindberg} and Bassard, {Jean-{\'E}tienne Andr{\'e}}",
note = "Copyright {\textcopyright} 2016, American Association for the Advancement of Science.",
year = "2016",
doi = "10.1126/science.aag2347",
language = "English",
volume = "354",
pages = "890--893",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6314",

}

RIS

TY - JOUR

T1 - Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

AU - Laursen, Tomas

AU - Borch-Jensen, Jonas

AU - Baden, Camilla Knudsen

AU - Bavishi, Krutika

AU - Torta, Federico

AU - Martens, Helle Juel

AU - Silvestro, Daniele

AU - Hatzakis, Nikos

AU - Wenk, Markus R

AU - Dafforn, Timothy R

AU - Olsen, Carl Erik

AU - Motawie, Mohammed Saddik

AU - Hamberger, Björn Robert

AU - Møller, Birger Lindberg

AU - Bassard, Jean-Étienne André

N1 - Copyright © 2016, American Association for the Advancement of Science.

PY - 2016

Y1 - 2016

N2 - Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.

AB - Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.

U2 - 10.1126/science.aag2347

DO - 10.1126/science.aag2347

M3 - Journal article

C2 - 27856908

VL - 354

SP - 890

EP - 893

JO - Science

JF - Science

SN - 0036-8075

IS - 6314

ER -

ID: 169101862