Assembly of dynamic P450-mediated metabolons - order versus chaos
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Assembly of dynamic P450-mediated metabolons - order versus chaos. / Bassard, Jean-Étienne André; Møller, Birger Lindberg; Laursen, Tomas.
In: Current Biology, Vol. 3, No. 1, 2017, p. 37-51.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Assembly of dynamic P450-mediated metabolons - order versus chaos
AU - Bassard, Jean-Étienne André
AU - Møller, Birger Lindberg
AU - Laursen, Tomas
PY - 2017
Y1 - 2017
N2 - PURPOSE OF REVIEW: We provide an overview of the current knowledge on cytochrome P450-mediated metabolism organized as metabolons and factors that facilitate their stabilization. Essential parameters will be discussed including those that are commonly disregarded using the dhurrin metabolon from Sorghum bicolor as a case study.RECENT FINDINGS: Sessile plants control their metabolism to prioritize their resources between growth and development, or defense. This requires fine-tuned complex dynamic regulation of the metabolic networks involved. Within the recent years, numerous studies point to the formation of dynamic metabolons playing a major role in controlling the metabolic fluxes within such networks.SUMMARY: We propose that P450s and their partners interact and associate dynamically with POR, which acts as a charging station possibly in concert with Cytb5. Solvent environment, lipid composition, and non-catalytic proteins guide metabolon formation and thereby activity, which have important implications for synthetic biology approaches aiming to produce high-value specialized metabolites in heterologous hosts.
AB - PURPOSE OF REVIEW: We provide an overview of the current knowledge on cytochrome P450-mediated metabolism organized as metabolons and factors that facilitate their stabilization. Essential parameters will be discussed including those that are commonly disregarded using the dhurrin metabolon from Sorghum bicolor as a case study.RECENT FINDINGS: Sessile plants control their metabolism to prioritize their resources between growth and development, or defense. This requires fine-tuned complex dynamic regulation of the metabolic networks involved. Within the recent years, numerous studies point to the formation of dynamic metabolons playing a major role in controlling the metabolic fluxes within such networks.SUMMARY: We propose that P450s and their partners interact and associate dynamically with POR, which acts as a charging station possibly in concert with Cytb5. Solvent environment, lipid composition, and non-catalytic proteins guide metabolon formation and thereby activity, which have important implications for synthetic biology approaches aiming to produce high-value specialized metabolites in heterologous hosts.
KW - Journal Article
KW - Review
U2 - 10.1007/s40610-017-0053-y
DO - 10.1007/s40610-017-0053-y
M3 - Review
C2 - 28255532
VL - 3
SP - 37
EP - 51
JO - Current Biology
JF - Current Biology
SN - 0960-9822
IS - 1
ER -
ID: 180759722