Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants. / Amari, Foued; Fettouche, Abdelmadjid; Samra, Mario Abou; Kefalas, Panagiotis; Kampranis, Sotirios; Makris, Antonios M.

In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 24, 2008, p. 11740-11751.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Amari, F, Fettouche, A, Samra, MA, Kefalas, P, Kampranis, S & Makris, AM 2008, 'Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants', Journal of Agricultural and Food Chemistry, vol. 56, no. 24, pp. 11740-11751. https://doi.org/10.1021/jf802829r

APA

Amari, F., Fettouche, A., Samra, M. A., Kefalas, P., Kampranis, S., & Makris, A. M. (2008). Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants. Journal of Agricultural and Food Chemistry, 56(24), 11740-11751. https://doi.org/10.1021/jf802829r

Vancouver

Amari F, Fettouche A, Samra MA, Kefalas P, Kampranis S, Makris AM. Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants. Journal of Agricultural and Food Chemistry. 2008;56(24):11740-11751. https://doi.org/10.1021/jf802829r

Author

Amari, Foued ; Fettouche, Abdelmadjid ; Samra, Mario Abou ; Kefalas, Panagiotis ; Kampranis, Sotirios ; Makris, Antonios M. / Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants. In: Journal of Agricultural and Food Chemistry. 2008 ; Vol. 56, No. 24. pp. 11740-11751.

Bibtex

@article{2c7ecfa9d03a4a95af783a7a996fae6b,
title = "Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants",
abstract = "To assess the capacity of small molecules to function as antioxidants in pathologic conditions, a set of yeast assays utilizing strains deficient in the antioxidant machinery was applied with measurements of reactive oxygen species (ROS), glutathione (GSH/GSSG), and induction of the stress responsive proteins oye2 and oye3. Yeast strains deficient in superoxide dismutase (Delta sod1), catalase A (Delta cta1), and double-deficient in Old Yellow enzyme 2 and glutathione reductase 1 (Delta oye2 glr1) were supplemented with ascorbic acid, beta-carotene, caffeic acid, or quercetin, subjected to pro-oxidant insult, and monitored for growth recovery. Ascorbic acid and caffeic acid protected cells under most circumstances, whereas beta-carotene and quercetin protection was highly context dependent, exhibiting protection in some cases and inhibition in others. Beta-carotene and quercetin elevated substantially endogenous levels of ROS in some yeast mutants. Quercetin supplementation increased significantly GSH and GSSG levels but could not maintain GSH levels in H(2)O(2)-exposed cells. Induction of the stress response machinery was manifested by the strong up-regulation of a chromosomally encoded OYE2-GFP fusion. In the case of quercetin, there was simultaneous induction of OYE3-GFP, which was previously shown to sensitize cells to H(2)O(2)-induced programmed cell death (PCD). Taken together, the results show that mutations in the antioxidant machinery affect significantly the capacity of dietary antioxidants to protect cells.",
keywords = "Antioxidants, Catalase, Glutathione, Microbial Viability, Mutation, Oxidative Stress, Reactive Oxygen Species, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Superoxide Dismutase",
author = "Foued Amari and Abdelmadjid Fettouche and Samra, {Mario Abou} and Panagiotis Kefalas and Sotirios Kampranis and Makris, {Antonios M}",
year = "2008",
doi = "10.1021/jf802829r",
language = "English",
volume = "56",
pages = "11740--11751",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "24",

}

RIS

TY - JOUR

T1 - Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants

AU - Amari, Foued

AU - Fettouche, Abdelmadjid

AU - Samra, Mario Abou

AU - Kefalas, Panagiotis

AU - Kampranis, Sotirios

AU - Makris, Antonios M

PY - 2008

Y1 - 2008

N2 - To assess the capacity of small molecules to function as antioxidants in pathologic conditions, a set of yeast assays utilizing strains deficient in the antioxidant machinery was applied with measurements of reactive oxygen species (ROS), glutathione (GSH/GSSG), and induction of the stress responsive proteins oye2 and oye3. Yeast strains deficient in superoxide dismutase (Delta sod1), catalase A (Delta cta1), and double-deficient in Old Yellow enzyme 2 and glutathione reductase 1 (Delta oye2 glr1) were supplemented with ascorbic acid, beta-carotene, caffeic acid, or quercetin, subjected to pro-oxidant insult, and monitored for growth recovery. Ascorbic acid and caffeic acid protected cells under most circumstances, whereas beta-carotene and quercetin protection was highly context dependent, exhibiting protection in some cases and inhibition in others. Beta-carotene and quercetin elevated substantially endogenous levels of ROS in some yeast mutants. Quercetin supplementation increased significantly GSH and GSSG levels but could not maintain GSH levels in H(2)O(2)-exposed cells. Induction of the stress response machinery was manifested by the strong up-regulation of a chromosomally encoded OYE2-GFP fusion. In the case of quercetin, there was simultaneous induction of OYE3-GFP, which was previously shown to sensitize cells to H(2)O(2)-induced programmed cell death (PCD). Taken together, the results show that mutations in the antioxidant machinery affect significantly the capacity of dietary antioxidants to protect cells.

AB - To assess the capacity of small molecules to function as antioxidants in pathologic conditions, a set of yeast assays utilizing strains deficient in the antioxidant machinery was applied with measurements of reactive oxygen species (ROS), glutathione (GSH/GSSG), and induction of the stress responsive proteins oye2 and oye3. Yeast strains deficient in superoxide dismutase (Delta sod1), catalase A (Delta cta1), and double-deficient in Old Yellow enzyme 2 and glutathione reductase 1 (Delta oye2 glr1) were supplemented with ascorbic acid, beta-carotene, caffeic acid, or quercetin, subjected to pro-oxidant insult, and monitored for growth recovery. Ascorbic acid and caffeic acid protected cells under most circumstances, whereas beta-carotene and quercetin protection was highly context dependent, exhibiting protection in some cases and inhibition in others. Beta-carotene and quercetin elevated substantially endogenous levels of ROS in some yeast mutants. Quercetin supplementation increased significantly GSH and GSSG levels but could not maintain GSH levels in H(2)O(2)-exposed cells. Induction of the stress response machinery was manifested by the strong up-regulation of a chromosomally encoded OYE2-GFP fusion. In the case of quercetin, there was simultaneous induction of OYE3-GFP, which was previously shown to sensitize cells to H(2)O(2)-induced programmed cell death (PCD). Taken together, the results show that mutations in the antioxidant machinery affect significantly the capacity of dietary antioxidants to protect cells.

KW - Antioxidants

KW - Catalase

KW - Glutathione

KW - Microbial Viability

KW - Mutation

KW - Oxidative Stress

KW - Reactive Oxygen Species

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Superoxide Dismutase

U2 - 10.1021/jf802829r

DO - 10.1021/jf802829r

M3 - Journal article

C2 - 19049288

VL - 56

SP - 11740

EP - 11751

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 24

ER -

ID: 159085074