A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination. / Miettinen, Karel; Leelahakorn, Nattawat; Almeida Robles, Aldo Ricardo; Zhao, Yong; Hansen, Lukas Ronild; Nikolajsen, Iben Egebæk; Andersen, Jens Bo; Givskov, Michael; Stærk, Dan; Bak, Søren; Kampranis, Sotirios.

In: Nature Communications, Vol. 13, 3664, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Miettinen, K, Leelahakorn, N, Almeida Robles, AR, Zhao, Y, Hansen, LR, Nikolajsen, IE, Andersen, JB, Givskov, M, Stærk, D, Bak, S & Kampranis, S 2022, 'A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination', Nature Communications, vol. 13, 3664. https://doi.org/10.1038/s41467-022-31357-6

APA

Miettinen, K., Leelahakorn, N., Almeida Robles, A. R., Zhao, Y., Hansen, L. R., Nikolajsen, I. E., Andersen, J. B., Givskov, M., Stærk, D., Bak, S., & Kampranis, S. (2022). A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination. Nature Communications, 13, [3664]. https://doi.org/10.1038/s41467-022-31357-6

Vancouver

Miettinen K, Leelahakorn N, Almeida Robles AR, Zhao Y, Hansen LR, Nikolajsen IE et al. A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination. Nature Communications. 2022;13. 3664. https://doi.org/10.1038/s41467-022-31357-6

Author

Miettinen, Karel ; Leelahakorn, Nattawat ; Almeida Robles, Aldo Ricardo ; Zhao, Yong ; Hansen, Lukas Ronild ; Nikolajsen, Iben Egebæk ; Andersen, Jens Bo ; Givskov, Michael ; Stærk, Dan ; Bak, Søren ; Kampranis, Sotirios. / A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination. In: Nature Communications. 2022 ; Vol. 13.

Bibtex

@article{e8b7bdc1af4547c4a63758b34961b2c9,
title = "A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination",
abstract = "The decriminalization of cannabis and the growing interest in cannabinoids as therapeutics require efficient methods to discover novel compounds and monitor cannabinoid levels in human samples and products. However, current methods are limited by the structural diversity of the active compounds. Here, we construct a G-protein coupled receptor-based yeast whole-cell biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three real-life applications. First, we screen a library of compounds to discover two novel agonists and four antagonists and demonstrate that our biosensor can democratize GPCR drug discovery by enabling low-cost high-throughput analysis using open-source automation. Subsequently, we bioprospect 54 plants to discover a novel phytocannabinoid, dugesialactone. Finally, we develop a robust portable device, analyze body-fluid samples, and confidently detect illicit synthetic drugs like “Spice”/“K2”. Taking advantage of the extensive sensing repertoire of GPCRs, this technology can be extended to detect numerous other compounds.",
author = "Karel Miettinen and Nattawat Leelahakorn and {Almeida Robles}, {Aldo Ricardo} and Yong Zhao and Hansen, {Lukas Ronild} and Nikolajsen, {Iben Egeb{\ae}k} and Andersen, {Jens Bo} and Michael Givskov and Dan St{\ae}rk and S{\o}ren Bak and Sotirios Kampranis",
year = "2022",
doi = "10.1038/s41467-022-31357-6",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination

AU - Miettinen, Karel

AU - Leelahakorn, Nattawat

AU - Almeida Robles, Aldo Ricardo

AU - Zhao, Yong

AU - Hansen, Lukas Ronild

AU - Nikolajsen, Iben Egebæk

AU - Andersen, Jens Bo

AU - Givskov, Michael

AU - Stærk, Dan

AU - Bak, Søren

AU - Kampranis, Sotirios

PY - 2022

Y1 - 2022

N2 - The decriminalization of cannabis and the growing interest in cannabinoids as therapeutics require efficient methods to discover novel compounds and monitor cannabinoid levels in human samples and products. However, current methods are limited by the structural diversity of the active compounds. Here, we construct a G-protein coupled receptor-based yeast whole-cell biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three real-life applications. First, we screen a library of compounds to discover two novel agonists and four antagonists and demonstrate that our biosensor can democratize GPCR drug discovery by enabling low-cost high-throughput analysis using open-source automation. Subsequently, we bioprospect 54 plants to discover a novel phytocannabinoid, dugesialactone. Finally, we develop a robust portable device, analyze body-fluid samples, and confidently detect illicit synthetic drugs like “Spice”/“K2”. Taking advantage of the extensive sensing repertoire of GPCRs, this technology can be extended to detect numerous other compounds.

AB - The decriminalization of cannabis and the growing interest in cannabinoids as therapeutics require efficient methods to discover novel compounds and monitor cannabinoid levels in human samples and products. However, current methods are limited by the structural diversity of the active compounds. Here, we construct a G-protein coupled receptor-based yeast whole-cell biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three real-life applications. First, we screen a library of compounds to discover two novel agonists and four antagonists and demonstrate that our biosensor can democratize GPCR drug discovery by enabling low-cost high-throughput analysis using open-source automation. Subsequently, we bioprospect 54 plants to discover a novel phytocannabinoid, dugesialactone. Finally, we develop a robust portable device, analyze body-fluid samples, and confidently detect illicit synthetic drugs like “Spice”/“K2”. Taking advantage of the extensive sensing repertoire of GPCRs, this technology can be extended to detect numerous other compounds.

U2 - 10.1038/s41467-022-31357-6

DO - 10.1038/s41467-022-31357-6

M3 - Journal article

C2 - 35760809

VL - 13

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 3664

ER -

ID: 310453814