Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii. / Gimpel, Javier A.; Nour-Eldin, Hussam Hassan; Scranton, Melissa A.; Li, Daphne; Mayfield, Stephen P.

In: A C S Synthetic Biology, Vol. 5, No. 7, 2016, p. 589-596.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Gimpel, JA, Nour-Eldin, HH, Scranton, MA, Li, D & Mayfield, SP 2016, 'Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii', A C S Synthetic Biology, vol. 5, no. 7, pp. 589-596. https://doi.org/10.1021/acssynbio.5b00076

APA

Gimpel, J. A., Nour-Eldin, H. H., Scranton, M. A., Li, D., & Mayfield, S. P. (2016). Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii. A C S Synthetic Biology, 5(7), 589-596. https://doi.org/10.1021/acssynbio.5b00076

Vancouver

Gimpel JA, Nour-Eldin HH, Scranton MA, Li D, Mayfield SP. Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii. A C S Synthetic Biology. 2016;5(7):589-596. https://doi.org/10.1021/acssynbio.5b00076

Author

Gimpel, Javier A. ; Nour-Eldin, Hussam Hassan ; Scranton, Melissa A. ; Li, Daphne ; Mayfield, Stephen P. / Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii. In: A C S Synthetic Biology. 2016 ; Vol. 5, No. 7. pp. 589-596.

Bibtex

@article{0c8605fa80cf419ab648f178b08599ed,
title = "Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii",
abstract = "Oxygenic photosynthesis provides the energy to produce all food and most of the fuel on this planet. Photosystem II (PSII) is an essential and rate-limiting component of this process. Understanding and modifying PSII function could provide an opportunity for optimizing photosynthetic biomass production, particularly under specific environmental conditions. PSII is a complex multisubunit enzyme with strong interdependence among its components. In this work, we have deleted the six core genes of PSII in the eukaryotic alga Chlamydomonas reinhardtii and refactored them in a single DNA construct. Complementation of the knockout strain with the core PSII synthetic module from three different green algae resulted in reconstitution of photosynthetic activity to 85, 55, and 53% of that of the wild-type, demonstrating that the PSII core can be exchanged between algae species and retain function. The strains, synthetic cassettes, and refactoring strategy developed for this study demonstrate the potential of synthetic biology approaches for tailoring oxygenic photosynthesis and provide a powerful tool for unraveling PSII structure-function relationships.",
author = "Gimpel, {Javier A.} and Nour-Eldin, {Hussam Hassan} and Scranton, {Melissa A.} and Daphne Li and Mayfield, {Stephen P.}",
year = "2016",
doi = "10.1021/acssynbio.5b00076",
language = "English",
volume = "5",
pages = "589--596",
journal = "ACS Synthetic Biology",
issn = "2161-5063",
publisher = "American Chemical Society",
number = "7",

}

RIS

TY - JOUR

T1 - Refactoring the six-gene photosystem II core in the chloroplast of the green algae Chlamydomonas reinhardtii

AU - Gimpel, Javier A.

AU - Nour-Eldin, Hussam Hassan

AU - Scranton, Melissa A.

AU - Li, Daphne

AU - Mayfield, Stephen P.

PY - 2016

Y1 - 2016

N2 - Oxygenic photosynthesis provides the energy to produce all food and most of the fuel on this planet. Photosystem II (PSII) is an essential and rate-limiting component of this process. Understanding and modifying PSII function could provide an opportunity for optimizing photosynthetic biomass production, particularly under specific environmental conditions. PSII is a complex multisubunit enzyme with strong interdependence among its components. In this work, we have deleted the six core genes of PSII in the eukaryotic alga Chlamydomonas reinhardtii and refactored them in a single DNA construct. Complementation of the knockout strain with the core PSII synthetic module from three different green algae resulted in reconstitution of photosynthetic activity to 85, 55, and 53% of that of the wild-type, demonstrating that the PSII core can be exchanged between algae species and retain function. The strains, synthetic cassettes, and refactoring strategy developed for this study demonstrate the potential of synthetic biology approaches for tailoring oxygenic photosynthesis and provide a powerful tool for unraveling PSII structure-function relationships.

AB - Oxygenic photosynthesis provides the energy to produce all food and most of the fuel on this planet. Photosystem II (PSII) is an essential and rate-limiting component of this process. Understanding and modifying PSII function could provide an opportunity for optimizing photosynthetic biomass production, particularly under specific environmental conditions. PSII is a complex multisubunit enzyme with strong interdependence among its components. In this work, we have deleted the six core genes of PSII in the eukaryotic alga Chlamydomonas reinhardtii and refactored them in a single DNA construct. Complementation of the knockout strain with the core PSII synthetic module from three different green algae resulted in reconstitution of photosynthetic activity to 85, 55, and 53% of that of the wild-type, demonstrating that the PSII core can be exchanged between algae species and retain function. The strains, synthetic cassettes, and refactoring strategy developed for this study demonstrate the potential of synthetic biology approaches for tailoring oxygenic photosynthesis and provide a powerful tool for unraveling PSII structure-function relationships.

U2 - 10.1021/acssynbio.5b00076

DO - 10.1021/acssynbio.5b00076

M3 - Journal article

C2 - 26214707

VL - 5

SP - 589

EP - 596

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

SN - 2161-5063

IS - 7

ER -

ID: 155938983