Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER

Research output: Contribution to journal › Journal article › Research › peer-review

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Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER. / Erstad, Simon Matthe; Sakuragi, Yumiko.

In: Bio-protocol, Vol. 8, No. 01, 2018, p. 1-10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Erstad, SM & Sakuragi, Y 2018, 'Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER', Bio-protocol, vol. 8, no. 01, pp. 1-10. https://doi.org/10.21769/BioProtoc.2667

APA

Erstad, S. M., & Sakuragi, Y. (2018). Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER. Bio-protocol, 8(01), 1-10. https://doi.org/10.21769/BioProtoc.2667

Vancouver

Erstad SM, Sakuragi Y. Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER. Bio-protocol. 2018;8(01):1-10. https://doi.org/10.21769/BioProtoc.2667

Author

Erstad, Simon Matthe ; Sakuragi, Yumiko. / Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER. In: Bio-protocol. 2018 ; Vol. 8, No. 01. pp. 1-10.

Bibtex

@article{0d5d987451754242a1ab568de32befd4,
title = "Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER",
abstract = "Cyanobacteria are photosynthetic bacteria that thrive in diverse ecosystems and play major roles in the global carbon cycle. The abilities of cyanobacteria to fix atmospheric CO2 and to allocate the fixed carbons to chemicals and biofuels have attracted growing attentions as sustainable microbial cell factories. A better understanding of activities of enzymes involved in the central carbon metabolism might lead to increased product yields. Currently, cell-free lysates are widely used for the determination of intracellular enzyme activities. However, due to thick cell walls in cyanobacteria, lysis of cyanobacterial cells is inefficient and often laborious. The present protocol describes an easy and efficient method to permeabilize cyanobacterial cells, without lysing them, and direct usage of the permeabilized cells for the determination of metabolic enzyme activities in vivo.",
author = "Erstad, {Simon Matthe} and Yumiko Sakuragi",
year = "2018",
doi = "10.21769/BioProtoc.2667",
language = "English",
volume = "8",
pages = "1--10",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "01",

}

RIS

TY - JOUR

T1 - Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER

AU - Erstad, Simon Matthe

AU - Sakuragi, Yumiko

PY - 2018

Y1 - 2018

N2 - Cyanobacteria are photosynthetic bacteria that thrive in diverse ecosystems and play major roles in the global carbon cycle. The abilities of cyanobacteria to fix atmospheric CO2 and to allocate the fixed carbons to chemicals and biofuels have attracted growing attentions as sustainable microbial cell factories. A better understanding of activities of enzymes involved in the central carbon metabolism might lead to increased product yields. Currently, cell-free lysates are widely used for the determination of intracellular enzyme activities. However, due to thick cell walls in cyanobacteria, lysis of cyanobacterial cells is inefficient and often laborious. The present protocol describes an easy and efficient method to permeabilize cyanobacterial cells, without lysing them, and direct usage of the permeabilized cells for the determination of metabolic enzyme activities in vivo.

AB - Cyanobacteria are photosynthetic bacteria that thrive in diverse ecosystems and play major roles in the global carbon cycle. The abilities of cyanobacteria to fix atmospheric CO2 and to allocate the fixed carbons to chemicals and biofuels have attracted growing attentions as sustainable microbial cell factories. A better understanding of activities of enzymes involved in the central carbon metabolism might lead to increased product yields. Currently, cell-free lysates are widely used for the determination of intracellular enzyme activities. However, due to thick cell walls in cyanobacteria, lysis of cyanobacterial cells is inefficient and often laborious. The present protocol describes an easy and efficient method to permeabilize cyanobacterial cells, without lysing them, and direct usage of the permeabilized cells for the determination of metabolic enzyme activities in vivo.

U2 - 10.21769/BioProtoc.2667

DO - 10.21769/BioProtoc.2667

M3 - Journal article

VL - 8

SP - 1

EP - 10

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 01

ER -

ID: 229374802