Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici

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Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici. / Yang, Fen; Yin, Qi.

In: Proteomics, Vol. 16, No. 1, 2016, p. 98-101.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yang, F & Yin, Q 2016, 'Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici', Proteomics, vol. 16, no. 1, pp. 98-101. https://doi.org/10.1002/pmic.201500168

APA

Yang, F., & Yin, Q. (2016). Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici. Proteomics, 16(1), 98-101. https://doi.org/10.1002/pmic.201500168

Vancouver

Yang F, Yin Q. Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici. Proteomics. 2016;16(1):98-101. https://doi.org/10.1002/pmic.201500168

Author

Yang, Fen ; Yin, Qi. / Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici. In: Proteomics. 2016 ; Vol. 16, No. 1. pp. 98-101.

Bibtex

@article{def2c7fffd6042f0b3d70c5b64ceeee7,
title = "Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici",
abstract = "Zymoseptoria tritici causes Septoria tritici blotch disease of wheat. To obtain a comprehensive protein dataset of this fungal pathogen, proteomes of Z. tritici growing in nutrient-limiting and rich media and in vivo at a late stage of wheat infection were fractionated by 1D gel or strong cation exchange (SCX) chromatography and analyzed by LC-MS/MS. A total of 5731, 5376 and 3168 Z. tritici proteins were confidently identified from these conditions, respectively. Of these in vitro and in planta proteins, 9 and 11% were predicted to contain signal peptides, respectively. Functional classification analysis revealed the proteins were involved in the various cellular activities. Comparison of three distinct protein expression profiles demonstrates the elevated carbohydrate, lipid and secondary metabolisms, transport, protein processing and energy production specifically in the host environment, in contrast to the enhancement of signaling, defense, replication, transcription and cell division in vitro. The data provide useful targets towards a better understanding of the molecular basis of Z. tritici growth, development, stress response and pathogenicity.",
keywords = "Ascomycota, Chromatography, Liquid, Fungal Proteins, Host-Pathogen Interactions, Plant Diseases, Proteome, Proteomics, Tandem Mass Spectrometry, Triticum, Journal Article, Research Support, Non-U.S. Gov't",
author = "Fen Yang and Qi Yin",
note = "{\textcopyright} 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2016",
doi = "10.1002/pmic.201500168",
language = "English",
volume = "16",
pages = "98--101",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "1",

}

RIS

TY - JOUR

T1 - Comprehensive proteomic analysis of the wheat pathogenic fungus Zymoseptoria tritici

AU - Yang, Fen

AU - Yin, Qi

N1 - © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2016

Y1 - 2016

N2 - Zymoseptoria tritici causes Septoria tritici blotch disease of wheat. To obtain a comprehensive protein dataset of this fungal pathogen, proteomes of Z. tritici growing in nutrient-limiting and rich media and in vivo at a late stage of wheat infection were fractionated by 1D gel or strong cation exchange (SCX) chromatography and analyzed by LC-MS/MS. A total of 5731, 5376 and 3168 Z. tritici proteins were confidently identified from these conditions, respectively. Of these in vitro and in planta proteins, 9 and 11% were predicted to contain signal peptides, respectively. Functional classification analysis revealed the proteins were involved in the various cellular activities. Comparison of three distinct protein expression profiles demonstrates the elevated carbohydrate, lipid and secondary metabolisms, transport, protein processing and energy production specifically in the host environment, in contrast to the enhancement of signaling, defense, replication, transcription and cell division in vitro. The data provide useful targets towards a better understanding of the molecular basis of Z. tritici growth, development, stress response and pathogenicity.

AB - Zymoseptoria tritici causes Septoria tritici blotch disease of wheat. To obtain a comprehensive protein dataset of this fungal pathogen, proteomes of Z. tritici growing in nutrient-limiting and rich media and in vivo at a late stage of wheat infection were fractionated by 1D gel or strong cation exchange (SCX) chromatography and analyzed by LC-MS/MS. A total of 5731, 5376 and 3168 Z. tritici proteins were confidently identified from these conditions, respectively. Of these in vitro and in planta proteins, 9 and 11% were predicted to contain signal peptides, respectively. Functional classification analysis revealed the proteins were involved in the various cellular activities. Comparison of three distinct protein expression profiles demonstrates the elevated carbohydrate, lipid and secondary metabolisms, transport, protein processing and energy production specifically in the host environment, in contrast to the enhancement of signaling, defense, replication, transcription and cell division in vitro. The data provide useful targets towards a better understanding of the molecular basis of Z. tritici growth, development, stress response and pathogenicity.

KW - Ascomycota

KW - Chromatography, Liquid

KW - Fungal Proteins

KW - Host-Pathogen Interactions

KW - Plant Diseases

KW - Proteome

KW - Proteomics

KW - Tandem Mass Spectrometry

KW - Triticum

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/pmic.201500168

DO - 10.1002/pmic.201500168

M3 - Journal article

C2 - 26435044

VL - 16

SP - 98

EP - 101

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 1

ER -

ID: 169137283