Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level.
Original language | English |
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Title of host publication | P-Type ATPases : methods and protocols |
Editors | Maike Bublitz |
Number of pages | 11 |
Volume | 2. |
Publisher | Springer |
Publication date | 2016 |
Pages | 181-191 |
Chapter | 18 |
ISBN (Print) | 978-1-4939-3178-1 |
ISBN (Electronic) | 978-1-4939-3179-8 |
DOIs | |
Publication status | Published - 2016 |
Series | Methods in Molecular Biology |
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Volume | 1377 |
ISSN | 1064-3745 |
- ATP-Binding Cassette Transporters, Adenosine Triphosphate, Biological Transport, Cell Membrane, Dithionite, Fluorescence, Lipids, Membrane Proteins, Molecular Biology, Proteolipids, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 169109211