Effects of soil protists on the antibiotic resistome under long term fertilization
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Effects of soil protists on the antibiotic resistome under long term fertilization. / Li, Hong Zhe; Zhu, Dong; Sun, An Qi; Qin, Yi Fei; Lindhardt, Jonathan Hessner; Cui, Li.
In: Environmental Pollution, Vol. 307, 119516, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effects of soil protists on the antibiotic resistome under long term fertilization
AU - Li, Hong Zhe
AU - Zhu, Dong
AU - Sun, An Qi
AU - Qin, Yi Fei
AU - Lindhardt, Jonathan Hessner
AU - Cui, Li
N1 - Publisher Copyright: © 2022 Elsevier Ltd
PY - 2022
Y1 - 2022
N2 - Soil protists are key in regulating soil microbial communities. However, our understanding on the role of soil protists in shaping antibiotic resistome is limited. Here, we considered the diversity and composition of bacteria, fungi and protists in arable soils collected from a long-term field experiment with multiple fertilization treatments. We explored the effects of soil protists on antibiotic resistome using high-throughput qPCR. Our results showed that long term fertilization had stronger effect on the composition of protists than those of bacteria and fungi. The detected number and relative abundance of antibiotic resistance genes (ARGs) were elevated in soils amended with organic fertilizer. Co-occurrence network analysis revealed that changes in protists may contribute to the changes in ARGs composition, and the application of different fertilizers altered the communities of protistan consumers, suggesting that effects of protistan communities on ARGs might be altered by the top-down impact on bacterial composition. This study demonstrates soil protists as promising agents in monitoring and regulating ecological risk of antibiotic resistome associated with organic fertilizers.
AB - Soil protists are key in regulating soil microbial communities. However, our understanding on the role of soil protists in shaping antibiotic resistome is limited. Here, we considered the diversity and composition of bacteria, fungi and protists in arable soils collected from a long-term field experiment with multiple fertilization treatments. We explored the effects of soil protists on antibiotic resistome using high-throughput qPCR. Our results showed that long term fertilization had stronger effect on the composition of protists than those of bacteria and fungi. The detected number and relative abundance of antibiotic resistance genes (ARGs) were elevated in soils amended with organic fertilizer. Co-occurrence network analysis revealed that changes in protists may contribute to the changes in ARGs composition, and the application of different fertilizers altered the communities of protistan consumers, suggesting that effects of protistan communities on ARGs might be altered by the top-down impact on bacterial composition. This study demonstrates soil protists as promising agents in monitoring and regulating ecological risk of antibiotic resistome associated with organic fertilizers.
KW - Antibiotic resistance genes
KW - Bioindicators
KW - Long term fertilization
KW - Network analysis
KW - Soil micro-food web
U2 - 10.1016/j.envpol.2022.119516
DO - 10.1016/j.envpol.2022.119516
M3 - Journal article
C2 - 35609845
AN - SCOPUS:85130534776
VL - 307
JO - Environmental Pollution
JF - Environmental Pollution
SN - 0269-7491
M1 - 119516
ER -
ID: 311611568