TY - JOUR

T1 - Can analyte protectants compensate wastewater matrix induced enhancement effects in gas chromatography – mass spectrometry analysis?

AU - Jørgensen, Mathias B.

AU - Christensen, Jan H.

N1 - Funding Information: This study is a contribution to the VANDALF project under grant agreement No. 9067-00032B and supported by the Innovation Fund Denmark. We would like to thank MSCi for making laboratory facilities and a GC-MS instrument available for our disposition. Furthermore, we would like to thank the reviewers for valuable suggestions, which improved the manuscript. Publisher Copyright: © 2022 The Author(s)

PY - 2022

Y1 - 2022

N2 - This study aimed to investigate the ability of analyte protectants to enhance GC-MS signals and compensate matrix effects for a range of micropollutants in pure standard, effluent, and influent wastewater samples during analysis and detection. Wastewater samples were prepared for analysis using multilayer solid phase extraction for the purpose of extracting sample components with a broad range of physical-chemical properties. The sample extracts were either spiked or not spiked with target compounds and four analyte protectants: 3-ethoxy-1,2-propanediol, D-sorbitol, gluconolactone, and shikimic acid. In this way, it was possible to evaluate the matrix effects of wastewater samples and compare the use of analyte protectants with the conventional correction method of allocating a best matching internal standard to each target compound. A relation was observed between level of wastewater treatment and matrix effects, with the largest effects observed for influent samples and the smallest effects for effluent samples. Compensation of matrix effects with analyte protectants gave comparable results with the conventional correction method of allocating a best matching internal standard to each of the 13 investigated micropollutants. The best overall compensation was observed using analyte protectants and the internal standard correction method in combination.

AB - This study aimed to investigate the ability of analyte protectants to enhance GC-MS signals and compensate matrix effects for a range of micropollutants in pure standard, effluent, and influent wastewater samples during analysis and detection. Wastewater samples were prepared for analysis using multilayer solid phase extraction for the purpose of extracting sample components with a broad range of physical-chemical properties. The sample extracts were either spiked or not spiked with target compounds and four analyte protectants: 3-ethoxy-1,2-propanediol, D-sorbitol, gluconolactone, and shikimic acid. In this way, it was possible to evaluate the matrix effects of wastewater samples and compare the use of analyte protectants with the conventional correction method of allocating a best matching internal standard to each target compound. A relation was observed between level of wastewater treatment and matrix effects, with the largest effects observed for influent samples and the smallest effects for effluent samples. Compensation of matrix effects with analyte protectants gave comparable results with the conventional correction method of allocating a best matching internal standard to each of the 13 investigated micropollutants. The best overall compensation was observed using analyte protectants and the internal standard correction method in combination.

KW - Analyte protectants

KW - GC-MS

KW - Matrix enhancement effects

KW - Micropollutants

KW - Wastewater

U2 - 10.1016/j.chroma.2022.463280

DO - 10.1016/j.chroma.2022.463280

M3 - Journal article

C2 - 35785676

AN - SCOPUS:85133218322

VL - 1676

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0301-4770

M1 - 463280

ER -