Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte

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Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte. / Hoffmann, Robert D; Palmgren, Michael Broberg.

In: Molecular Plant, Vol. 6, No. 4, 2013, p. 1176-1186.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hoffmann, RD & Palmgren, MB 2013, 'Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte', Molecular Plant, vol. 6, no. 4, pp. 1176-1186. https://doi.org/10.1093/mp/sst100

APA

Hoffmann, R. D., & Palmgren, M. B. (2013). Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte. Molecular Plant, 6(4), 1176-1186. https://doi.org/10.1093/mp/sst100

Vancouver

Hoffmann RD, Palmgren MB. Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte. Molecular Plant. 2013;6(4):1176-1186. https://doi.org/10.1093/mp/sst100

Author

Hoffmann, Robert D ; Palmgren, Michael Broberg. / Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte. In: Molecular Plant. 2013 ; Vol. 6, No. 4. pp. 1176-1186.

Bibtex

@article{cacf8aa6ae5543d68852c06fafdccf7c,
title = "Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte",
abstract = "Tissue formation, the identity of cells, and the functions they fulfill, are results of gene regulation. The male gametophyte of plants, pollen, is outstanding in this respect as several hundred genes expressed in pollen are not expressed in the sporophyte. How pollen-specific genes are down-regulated in the sporophyte has yet to be established. In this study, we have performed a bioinformatics analysis of publicly available genome-wide epigenetics data of several sporophytic tissues. By combining this analysis with DNase I footprinting data, we assessed means by which the repression of pollen-specific genes in the Arabidopsis sporophyte is conferred. Our findings show that, in seedlings, the majority of pollen-specific genes are associated with histone-3 marked by mono- or trimethylation of Lys-27 (H3K27me1/H3K27me3), both of which are repressive markers for gene expression in the sporophyte. Analysis of DNase footprint profiles of pollen-specific genes in the sporophyte displayed closed chromatin proximal to the start codon. We describe a model of two-staged gene regulation in which a lack of nucleosome-free regions in promoters and histone modifications in open reading frames repress pollen-specific genes in the sporophyte.",
author = "Hoffmann, {Robert D} and Palmgren, {Michael Broberg}",
year = "2013",
doi = "10.1093/mp/sst100",
language = "English",
volume = "6",
pages = "1176--1186",
journal = "Molecular Plant",
issn = "1674-2052",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte

AU - Hoffmann, Robert D

AU - Palmgren, Michael Broberg

PY - 2013

Y1 - 2013

N2 - Tissue formation, the identity of cells, and the functions they fulfill, are results of gene regulation. The male gametophyte of plants, pollen, is outstanding in this respect as several hundred genes expressed in pollen are not expressed in the sporophyte. How pollen-specific genes are down-regulated in the sporophyte has yet to be established. In this study, we have performed a bioinformatics analysis of publicly available genome-wide epigenetics data of several sporophytic tissues. By combining this analysis with DNase I footprinting data, we assessed means by which the repression of pollen-specific genes in the Arabidopsis sporophyte is conferred. Our findings show that, in seedlings, the majority of pollen-specific genes are associated with histone-3 marked by mono- or trimethylation of Lys-27 (H3K27me1/H3K27me3), both of which are repressive markers for gene expression in the sporophyte. Analysis of DNase footprint profiles of pollen-specific genes in the sporophyte displayed closed chromatin proximal to the start codon. We describe a model of two-staged gene regulation in which a lack of nucleosome-free regions in promoters and histone modifications in open reading frames repress pollen-specific genes in the sporophyte.

AB - Tissue formation, the identity of cells, and the functions they fulfill, are results of gene regulation. The male gametophyte of plants, pollen, is outstanding in this respect as several hundred genes expressed in pollen are not expressed in the sporophyte. How pollen-specific genes are down-regulated in the sporophyte has yet to be established. In this study, we have performed a bioinformatics analysis of publicly available genome-wide epigenetics data of several sporophytic tissues. By combining this analysis with DNase I footprinting data, we assessed means by which the repression of pollen-specific genes in the Arabidopsis sporophyte is conferred. Our findings show that, in seedlings, the majority of pollen-specific genes are associated with histone-3 marked by mono- or trimethylation of Lys-27 (H3K27me1/H3K27me3), both of which are repressive markers for gene expression in the sporophyte. Analysis of DNase footprint profiles of pollen-specific genes in the sporophyte displayed closed chromatin proximal to the start codon. We describe a model of two-staged gene regulation in which a lack of nucleosome-free regions in promoters and histone modifications in open reading frames repress pollen-specific genes in the sporophyte.

U2 - 10.1093/mp/sst100

DO - 10.1093/mp/sst100

M3 - Journal article

C2 - 23770838

VL - 6

SP - 1176

EP - 1186

JO - Molecular Plant

JF - Molecular Plant

SN - 1674-2052

IS - 4

ER -

ID: 86251887