Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts

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Standard

Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts. / Trinh, Mai Duy Luu; Sato, Ryoichi; Masuda, Shinji.

In: Photosynthesis Research, Vol. 139, No. 1-3, 2019, p. 413-424.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Trinh, MDL, Sato, R & Masuda, S 2019, 'Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts', Photosynthesis Research, vol. 139, no. 1-3, pp. 413-424. https://doi.org/10.1007/s11120-018-0575-z

APA

Trinh, M. D. L., Sato, R., & Masuda, S. (2019). Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts. Photosynthesis Research, 139(1-3), 413-424. https://doi.org/10.1007/s11120-018-0575-z

Vancouver

Trinh MDL, Sato R, Masuda S. Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts. Photosynthesis Research. 2019;139(1-3):413-424. https://doi.org/10.1007/s11120-018-0575-z

Author

Trinh, Mai Duy Luu ; Sato, Ryoichi ; Masuda, Shinji. / Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts. In: Photosynthesis Research. 2019 ; Vol. 139, No. 1-3. pp. 413-424.

Bibtex

@article{0405ae2b39c84714a1f6ac46e3399640,
title = "Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts",
abstract = "Precise control of the proton concentration gradient across thylakoid membranes (ΔpH) is essential for photosynthesis and its regulation because the gradient contributes to the generation of the proton motive force used for ATP synthesis and also for the fast and reversible induction of non-photochemical quenching (NPQ) to avoid photoinhibition and photodamage. However, the regulatory mechanism(s) controlling ΔpH in response to fluctuating light has not been fully elucidated. We previously described a new NPQ-regulatory chloroplastic protein, Fluctuating-Light-Acclimation Protein1 (FLAP1), which is important for plant growth and modulation of ΔpH under fluctuating light conditions. For this report, we further characterized FLAP1 activity by individually crossing an Arabidopsis flap1 mutant with npq4 and pgr5 plants; npq4 is defective in PsbS-dependent NPQ, and pgr5 is defective in induction of steady-state proton motive force (pmf) and energy-dependent quenching (qE). Both npq4 and npq4 flap1 exhibited similar NPQ kinetics and other photosynthetic parameters under constant or fluctuating actinic light. Conversely, pgr5 flap1 had recovered NPQ, photosystem II quantum yield and growth under fluctuating light, each of which was impaired in pgr5. Together with other data, we propose that FLAP1 activity controls proton homeostasis under steady-state photosynthesis to manipulate luminal acidification levels appropriately to balance photoprotection and photochemical processes.",
keywords = "FLAP1, Fluctuating light, Luminal acidification, Non-photochemical quenching, PGR5, Photosynthesis",
author = "Trinh, {Mai Duy Luu} and Ryoichi Sato and Shinji Masuda",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Nature B.V.",
year = "2019",
doi = "10.1007/s11120-018-0575-z",
language = "English",
volume = "139",
pages = "413--424",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer",
number = "1-3",

}

RIS

TY - JOUR

T1 - Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts

AU - Trinh, Mai Duy Luu

AU - Sato, Ryoichi

AU - Masuda, Shinji

N1 - Publisher Copyright: © 2018, Springer Nature B.V.

PY - 2019

Y1 - 2019

N2 - Precise control of the proton concentration gradient across thylakoid membranes (ΔpH) is essential for photosynthesis and its regulation because the gradient contributes to the generation of the proton motive force used for ATP synthesis and also for the fast and reversible induction of non-photochemical quenching (NPQ) to avoid photoinhibition and photodamage. However, the regulatory mechanism(s) controlling ΔpH in response to fluctuating light has not been fully elucidated. We previously described a new NPQ-regulatory chloroplastic protein, Fluctuating-Light-Acclimation Protein1 (FLAP1), which is important for plant growth and modulation of ΔpH under fluctuating light conditions. For this report, we further characterized FLAP1 activity by individually crossing an Arabidopsis flap1 mutant with npq4 and pgr5 plants; npq4 is defective in PsbS-dependent NPQ, and pgr5 is defective in induction of steady-state proton motive force (pmf) and energy-dependent quenching (qE). Both npq4 and npq4 flap1 exhibited similar NPQ kinetics and other photosynthetic parameters under constant or fluctuating actinic light. Conversely, pgr5 flap1 had recovered NPQ, photosystem II quantum yield and growth under fluctuating light, each of which was impaired in pgr5. Together with other data, we propose that FLAP1 activity controls proton homeostasis under steady-state photosynthesis to manipulate luminal acidification levels appropriately to balance photoprotection and photochemical processes.

AB - Precise control of the proton concentration gradient across thylakoid membranes (ΔpH) is essential for photosynthesis and its regulation because the gradient contributes to the generation of the proton motive force used for ATP synthesis and also for the fast and reversible induction of non-photochemical quenching (NPQ) to avoid photoinhibition and photodamage. However, the regulatory mechanism(s) controlling ΔpH in response to fluctuating light has not been fully elucidated. We previously described a new NPQ-regulatory chloroplastic protein, Fluctuating-Light-Acclimation Protein1 (FLAP1), which is important for plant growth and modulation of ΔpH under fluctuating light conditions. For this report, we further characterized FLAP1 activity by individually crossing an Arabidopsis flap1 mutant with npq4 and pgr5 plants; npq4 is defective in PsbS-dependent NPQ, and pgr5 is defective in induction of steady-state proton motive force (pmf) and energy-dependent quenching (qE). Both npq4 and npq4 flap1 exhibited similar NPQ kinetics and other photosynthetic parameters under constant or fluctuating actinic light. Conversely, pgr5 flap1 had recovered NPQ, photosystem II quantum yield and growth under fluctuating light, each of which was impaired in pgr5. Together with other data, we propose that FLAP1 activity controls proton homeostasis under steady-state photosynthesis to manipulate luminal acidification levels appropriately to balance photoprotection and photochemical processes.

KW - FLAP1

KW - Fluctuating light

KW - Luminal acidification

KW - Non-photochemical quenching

KW - PGR5

KW - Photosynthesis

U2 - 10.1007/s11120-018-0575-z

DO - 10.1007/s11120-018-0575-z

M3 - Journal article

C2 - 30390180

AN - SCOPUS:85055986295

VL - 139

SP - 413

EP - 424

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 1-3

ER -

ID: 386068276