VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are diffi cult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.
Original language | English |
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Title of host publication | Plant gene silencing : methods and protocols |
Editors | Kirankumar S. Mysore, Muthappa Senthil-Kumar |
Number of pages | 11 |
Volume | Part II |
Publisher | Springer |
Publication date | 2015 |
Pages | 255-265 |
Chapter | 19 |
ISBN (Print) | 978-1-4939-2452-3 |
ISBN (Electronic) | 978-1-4939-2453-0 |
DOIs | |
Publication status | Published - 2015 |
Series | Methods in Molecular Biology |
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Volume | 1287 |
ISSN | 1064-3745 |
- Arbuscular mycorrhizal symbiosis, Legume- Rhizobium symbiosis, PEBV, VIGS
Research areas
ID: 160099614