Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat

Research output: Contribution to journalJournal articleResearchpeer-review

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Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat. / Rojas, Edward C.; Jensen, Birgit; Jørgensen, Hans J.L.; Latz, Meike A.C.; Esteban, Pilar; Ding, Yuwei; Collinge, David B.

In: Biological Control, Vol. 144, 104222, 05.2020.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rojas, EC, Jensen, B, Jørgensen, HJL, Latz, MAC, Esteban, P, Ding, Y & Collinge, DB 2020, 'Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat', Biological Control, vol. 144, 104222. https://doi.org/10.1016/j.biocontrol.2020.104222

APA

Rojas, E. C., Jensen, B., Jørgensen, H. J. L., Latz, M. A. C., Esteban, P., Ding, Y., & Collinge, D. B. (2020). Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat. Biological Control, 144, [104222]. https://doi.org/10.1016/j.biocontrol.2020.104222

Vancouver

Rojas EC, Jensen B, Jørgensen HJL, Latz MAC, Esteban P, Ding Y et al. Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat. Biological Control. 2020 May;144. 104222. https://doi.org/10.1016/j.biocontrol.2020.104222

Author

Rojas, Edward C. ; Jensen, Birgit ; Jørgensen, Hans J.L. ; Latz, Meike A.C. ; Esteban, Pilar ; Ding, Yuwei ; Collinge, David B. / Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat. In: Biological Control. 2020 ; Vol. 144.

Bibtex

@article{595a38a5c2384b0583cc406adc7451ec,
title = "Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat",
abstract = "Fusarium head blight (FHB) is a major disease in wheat and other cereal crops. Biological control is a promising alternative for control of this disease as current control options are not efficient. Fungal endophytes could represent a new source of biological control agents since they colonise plant tissues asymptomatically whilst sharing the same biological niche as pathogens. Here, we show that healthy wheat spikes and leaves collected in areas with high FHB incidence harbour fungal isolates that reduce disease severity and pathogen biomass inside the spikes. We employed an in planta screening method using detached spikelets to observe the performance of 15 fungal strains against Fusarium infection. The results were validated in greenhouse spike inoculation assays. Isolates Sarocladium strictum C113L, Anthracocystis floculossa P1P1, A. floculossa F63P and Penicillium olsonii ML37 were identified as potential biocontrol agents of FHB in wheat. Timing of application was important: the endophytes needed to be applied at least two days before the pathogen to have a significant effect. Reduced symptom expression correlated with reduced biomass of Fusarium graminearum in the infected spikelets. Interestingly, none of these isolates reduced Fusarium growth in confrontation assays on agar plates. Our results suggest that naturally occurring endophytes could be a new source of biological control agents to be used in integrated pest management strategies.",
keywords = "Anthracocystis floculossa, Endophyte isolation, Penicillium olsonii, Sarocladium strictum, Screening",
author = "Rojas, {Edward C.} and Birgit Jensen and J{\o}rgensen, {Hans J.L.} and Latz, {Meike A.C.} and Pilar Esteban and Yuwei Ding and Collinge, {David B.}",
year = "2020",
month = "5",
doi = "10.1016/j.biocontrol.2020.104222",
language = "English",
volume = "144",
journal = "Biological Control",
issn = "1049-9644",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat

AU - Rojas, Edward C.

AU - Jensen, Birgit

AU - Jørgensen, Hans J.L.

AU - Latz, Meike A.C.

AU - Esteban, Pilar

AU - Ding, Yuwei

AU - Collinge, David B.

PY - 2020/5

Y1 - 2020/5

N2 - Fusarium head blight (FHB) is a major disease in wheat and other cereal crops. Biological control is a promising alternative for control of this disease as current control options are not efficient. Fungal endophytes could represent a new source of biological control agents since they colonise plant tissues asymptomatically whilst sharing the same biological niche as pathogens. Here, we show that healthy wheat spikes and leaves collected in areas with high FHB incidence harbour fungal isolates that reduce disease severity and pathogen biomass inside the spikes. We employed an in planta screening method using detached spikelets to observe the performance of 15 fungal strains against Fusarium infection. The results were validated in greenhouse spike inoculation assays. Isolates Sarocladium strictum C113L, Anthracocystis floculossa P1P1, A. floculossa F63P and Penicillium olsonii ML37 were identified as potential biocontrol agents of FHB in wheat. Timing of application was important: the endophytes needed to be applied at least two days before the pathogen to have a significant effect. Reduced symptom expression correlated with reduced biomass of Fusarium graminearum in the infected spikelets. Interestingly, none of these isolates reduced Fusarium growth in confrontation assays on agar plates. Our results suggest that naturally occurring endophytes could be a new source of biological control agents to be used in integrated pest management strategies.

AB - Fusarium head blight (FHB) is a major disease in wheat and other cereal crops. Biological control is a promising alternative for control of this disease as current control options are not efficient. Fungal endophytes could represent a new source of biological control agents since they colonise plant tissues asymptomatically whilst sharing the same biological niche as pathogens. Here, we show that healthy wheat spikes and leaves collected in areas with high FHB incidence harbour fungal isolates that reduce disease severity and pathogen biomass inside the spikes. We employed an in planta screening method using detached spikelets to observe the performance of 15 fungal strains against Fusarium infection. The results were validated in greenhouse spike inoculation assays. Isolates Sarocladium strictum C113L, Anthracocystis floculossa P1P1, A. floculossa F63P and Penicillium olsonii ML37 were identified as potential biocontrol agents of FHB in wheat. Timing of application was important: the endophytes needed to be applied at least two days before the pathogen to have a significant effect. Reduced symptom expression correlated with reduced biomass of Fusarium graminearum in the infected spikelets. Interestingly, none of these isolates reduced Fusarium growth in confrontation assays on agar plates. Our results suggest that naturally occurring endophytes could be a new source of biological control agents to be used in integrated pest management strategies.

KW - Anthracocystis floculossa

KW - Endophyte isolation

KW - Penicillium olsonii

KW - Sarocladium strictum

KW - Screening

UR - http://www.scopus.com/inward/record.url?scp=85081003751&partnerID=8YFLogxK

U2 - 10.1016/j.biocontrol.2020.104222

DO - 10.1016/j.biocontrol.2020.104222

M3 - Journal article

AN - SCOPUS:85081003751

VL - 144

JO - Biological Control

JF - Biological Control

SN - 1049-9644

M1 - 104222

ER -

ID: 239818024