Immunoelectrophoretic studies on pig intestinal brush border proteins

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Standard

Immunoelectrophoretic studies on pig intestinal brush border proteins. / Danielsen, Erik Michael; Sjöström, H; Norén, O; Dabelsteen, E.

In: BBA General Subjects, Vol. 494, No. 2, 1977, p. 332-42.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Danielsen, EM, Sjöström, H, Norén, O & Dabelsteen, E 1977, 'Immunoelectrophoretic studies on pig intestinal brush border proteins', BBA General Subjects, vol. 494, no. 2, pp. 332-42.

APA

Danielsen, E. M., Sjöström, H., Norén, O., & Dabelsteen, E. (1977). Immunoelectrophoretic studies on pig intestinal brush border proteins. BBA General Subjects, 494(2), 332-42.

Vancouver

Danielsen EM, Sjöström H, Norén O, Dabelsteen E. Immunoelectrophoretic studies on pig intestinal brush border proteins. BBA General Subjects. 1977;494(2):332-42.

Author

Danielsen, Erik Michael ; Sjöström, H ; Norén, O ; Dabelsteen, E. / Immunoelectrophoretic studies on pig intestinal brush border proteins. In: BBA General Subjects. 1977 ; Vol. 494, No. 2. pp. 332-42.

Bibtex

@article{88e1e1306c7d11de8bc9000ea68e967b,
title = "Immunoelectrophoretic studies on pig intestinal brush border proteins",
abstract = "Brush borders were prepared from pig intestinal mucosa and the membrane proteins solubilized with either Triton X-100 or papain. Proteins, thus released, were used as antigens to raise antisera in rabbits. The immunoglobulin G fractions were isolated and shown by the double layer immunofluorescence staining technique to react only with the brush border region of the enterocyte. The antibodies obtained were used in immunoelectrophoretic studies on the brush border proteins. Eight hydrolytic activities were identified by the use of histo-chemical staining methods. These were the microsomal aminopeptidase (EC 3.4.11.2), aspartate aminopeptidase (EC 3.4.11.7), dipeptidyl peptidase IV (EC 3.4.14.X), lactase (EC 3.2.1.23), glucoamylase (EC 3.2.1.3), sucrase (EC 3.2.1.48), isomaltase (EC 3.2.1.10) and alkaline phosphatase (EC 3.1.3.1). In addition, at least four faint immunoprecipitates were formed but none of these were identified.",
author = "Danielsen, {Erik Michael} and H Sj{\"o}str{\"o}m and O Nor{\'e}n and E Dabelsteen",
note = "Keywords: Alkaline Phosphatase; Aminopeptidases; Animals; Antibody Specificity; Endopeptidases; Glucan 1,4-alpha-Glucosidase; Immunoelectrophoresis, Two-Dimensional; Intestinal Mucosa; Intestine, Small; Microvilli; Polyethylene Glycols; Sucrase-Isomaltase Complex; Swine; beta-Galactosidase; gamma-Glutamyltransferase",
year = "1977",
language = "English",
volume = "494",
pages = "332--42",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Immunoelectrophoretic studies on pig intestinal brush border proteins

AU - Danielsen, Erik Michael

AU - Sjöström, H

AU - Norén, O

AU - Dabelsteen, E

N1 - Keywords: Alkaline Phosphatase; Aminopeptidases; Animals; Antibody Specificity; Endopeptidases; Glucan 1,4-alpha-Glucosidase; Immunoelectrophoresis, Two-Dimensional; Intestinal Mucosa; Intestine, Small; Microvilli; Polyethylene Glycols; Sucrase-Isomaltase Complex; Swine; beta-Galactosidase; gamma-Glutamyltransferase

PY - 1977

Y1 - 1977

N2 - Brush borders were prepared from pig intestinal mucosa and the membrane proteins solubilized with either Triton X-100 or papain. Proteins, thus released, were used as antigens to raise antisera in rabbits. The immunoglobulin G fractions were isolated and shown by the double layer immunofluorescence staining technique to react only with the brush border region of the enterocyte. The antibodies obtained were used in immunoelectrophoretic studies on the brush border proteins. Eight hydrolytic activities were identified by the use of histo-chemical staining methods. These were the microsomal aminopeptidase (EC 3.4.11.2), aspartate aminopeptidase (EC 3.4.11.7), dipeptidyl peptidase IV (EC 3.4.14.X), lactase (EC 3.2.1.23), glucoamylase (EC 3.2.1.3), sucrase (EC 3.2.1.48), isomaltase (EC 3.2.1.10) and alkaline phosphatase (EC 3.1.3.1). In addition, at least four faint immunoprecipitates were formed but none of these were identified.

AB - Brush borders were prepared from pig intestinal mucosa and the membrane proteins solubilized with either Triton X-100 or papain. Proteins, thus released, were used as antigens to raise antisera in rabbits. The immunoglobulin G fractions were isolated and shown by the double layer immunofluorescence staining technique to react only with the brush border region of the enterocyte. The antibodies obtained were used in immunoelectrophoretic studies on the brush border proteins. Eight hydrolytic activities were identified by the use of histo-chemical staining methods. These were the microsomal aminopeptidase (EC 3.4.11.2), aspartate aminopeptidase (EC 3.4.11.7), dipeptidyl peptidase IV (EC 3.4.14.X), lactase (EC 3.2.1.23), glucoamylase (EC 3.2.1.3), sucrase (EC 3.2.1.48), isomaltase (EC 3.2.1.10) and alkaline phosphatase (EC 3.1.3.1). In addition, at least four faint immunoprecipitates were formed but none of these were identified.

M3 - Journal article

C2 - 20974

VL - 494

SP - 332

EP - 342

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 2

ER -

ID: 13063795