The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation
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The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation. / Harris, Abigail K P; Williamson, Neil R; Slater, Holly; Cox, Anthony; Abbasi, Sophia; Foulds, Ian; Simonsen, Henrik Toft; Leeper, Finian J; Salmond, George P C.
In: Microbiology, Vol. 150, No. Pt 11, 11.2004, p. 3547-60.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation
AU - Harris, Abigail K P
AU - Williamson, Neil R
AU - Slater, Holly
AU - Cox, Anthony
AU - Abbasi, Sophia
AU - Foulds, Ian
AU - Simonsen, Henrik Toft
AU - Leeper, Finian J
AU - Salmond, George P C
PY - 2004/11
Y1 - 2004/11
N2 - The prodigiosin biosynthesis gene cluster (pig cluster) from two strains of Serratia (S. marcescens ATCC 274 and Serratia sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective pig clusters revealed 14 ORFs in S. marcescens ATCC 274 and 15 ORFs in Serratia sp. ATCC 39006. In each Serratia species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of Streptomyces coelicolor A3(2). Comparisons between the two Serratia pig clusters and the red cluster from Str. coelicolor A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprodigiosin, is proposed. The distribution of the pig cluster within several Serratia sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The pig cluster of Serratia marcescens ATCC 274 is flanked by cueR and copA homologues and this configuration is demonstrated in several S. marcescens strains, whilst these genes are contiguous in strains lacking the pig cluster.
AB - The prodigiosin biosynthesis gene cluster (pig cluster) from two strains of Serratia (S. marcescens ATCC 274 and Serratia sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective pig clusters revealed 14 ORFs in S. marcescens ATCC 274 and 15 ORFs in Serratia sp. ATCC 39006. In each Serratia species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of Streptomyces coelicolor A3(2). Comparisons between the two Serratia pig clusters and the red cluster from Str. coelicolor A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprodigiosin, is proposed. The distribution of the pig cluster within several Serratia sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The pig cluster of Serratia marcescens ATCC 274 is flanked by cueR and copA homologues and this configuration is demonstrated in several S. marcescens strains, whilst these genes are contiguous in strains lacking the pig cluster.
KW - Bacterial Proteins
KW - Blotting, Southern
KW - DNA Fingerprinting
KW - DNA, Bacterial
KW - DNA-Binding Proteins
KW - Gene Order
KW - Genes, Bacterial
KW - Genetic Variation
KW - Molecular Sequence Data
KW - Multigene Family
KW - Open Reading Frames
KW - Peptide Synthases
KW - Polyketide Synthases
KW - Prodigiosin
KW - Sequence Analysis, DNA
KW - Sequence Homology, Amino Acid
KW - Serratia
KW - Streptomyces coelicolor
U2 - 10.1099/mic.0.27222-0
DO - 10.1099/mic.0.27222-0
M3 - Journal article
C2 - 15528645
VL - 150
SP - 3547
EP - 3560
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - Pt 11
ER -
ID: 128571127