Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
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Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations. / Chiurazzi, Maurizio Junior; Nørrevang, Anton Frisgaard; Garcia, Pedro; Cerdán, Pablo D.; Palmgren, Michael Broberg; Wenkel, Stephan.
In: Journal of Integrative Plant Biology, Vol. 64, No. 2, 2022, p. 205-214.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
AU - Chiurazzi, Maurizio Junior
AU - Nørrevang, Anton Frisgaard
AU - Garcia, Pedro
AU - Cerdán, Pablo D.
AU - Palmgren, Michael Broberg
AU - Wenkel, Stephan
N1 - This article is protected by copyright. All rights reserved. Special 70th anniversary issue: Invited expert reviews highlighting progress in plant biology research
PY - 2022
Y1 - 2022
N2 - Breeding plants with polyploid genomes is challenging because functional redundancy hampers the identification of loss-of-function mutants. Medicago sativa is tetraploid and obligate outcrossing, which together with inbreeding depression complicates traditional breeding approaches in obtaining plants with a stable growth habit. Inducing dominant mutations would provide an alternative strategy to introduce domestication traits in plants with high gene redundancy. Here we describe two complementary strategies to induce dominant mutations in the M. sativa genome and how they can be relevant in the control of flowering time. First, we outline a genome-engineering strategy that harnesses the use of microProteins as developmental regulators. MicroProteins are small proteins that appeared during genome evolution from genes encoding larger proteins. Genome-engineering allows us to retrace evolution and create microProtein-coding genes de novo. Second, we provide an inventory of genes regulated by microRNAs that control plant development. Making respective gene transcripts microRNA-resistant by inducing point mutations can uncouple microRNA regulation. Finally, we investigated the recently published genomes of M. sativa and provide an inventory of breeding targets, some of which, when mutated, are likely to result in dominant traits. This article is protected by copyright. All rights reserved.
AB - Breeding plants with polyploid genomes is challenging because functional redundancy hampers the identification of loss-of-function mutants. Medicago sativa is tetraploid and obligate outcrossing, which together with inbreeding depression complicates traditional breeding approaches in obtaining plants with a stable growth habit. Inducing dominant mutations would provide an alternative strategy to introduce domestication traits in plants with high gene redundancy. Here we describe two complementary strategies to induce dominant mutations in the M. sativa genome and how they can be relevant in the control of flowering time. First, we outline a genome-engineering strategy that harnesses the use of microProteins as developmental regulators. MicroProteins are small proteins that appeared during genome evolution from genes encoding larger proteins. Genome-engineering allows us to retrace evolution and create microProtein-coding genes de novo. Second, we provide an inventory of genes regulated by microRNAs that control plant development. Making respective gene transcripts microRNA-resistant by inducing point mutations can uncouple microRNA regulation. Finally, we investigated the recently published genomes of M. sativa and provide an inventory of breeding targets, some of which, when mutated, are likely to result in dominant traits. This article is protected by copyright. All rights reserved.
U2 - 10.1111/jipb.13186
DO - 10.1111/jipb.13186
M3 - Journal article
C2 - 34761872
VL - 64
SP - 205
EP - 214
JO - Journal of Integrative Plant Biology
JF - Journal of Integrative Plant Biology
SN - 1672-9072
IS - 2
ER -
ID: 285376006