Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses
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Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses. / Ryu, Moon Young; Cho, Seok Keun; Hong, Yourae; Kim, Jinho; Kim, Jong Hum; Kim, Gu Min; Chen, Yan-Jun; Knoch, Eva; Møller, Birger Lindberg; Kim, Woo Taek; Lyngkjær, Michael Foged; Yang, Seong Wook.
In: BMC Genomics, Vol. 20, 326, 29.04.2019, p. 1-15.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses
AU - Ryu, Moon Young
AU - Cho, Seok Keun
AU - Hong, Yourae
AU - Kim, Jinho
AU - Kim, Jong Hum
AU - Kim, Gu Min
AU - Chen, Yan-Jun
AU - Knoch, Eva
AU - Møller, Birger Lindberg
AU - Kim, Woo Taek
AU - Lyngkjær, Michael Foged
AU - Yang, Seong Wook
PY - 2019/4/29
Y1 - 2019/4/29
N2 - Background: Controlled turnover of proteins as mediated by the ubiquitin proteasome system (UPS) is an important element in plant defense against environmental and pathogen stresses. E3 ligases play a central role in subjecting proteins to hydrolysis by the UPS. Recently, it has been demonstrated that a specific class of E3 ligases termed the U-box ligases are directly associated with the defense mechanisms against abiotic and biotic stresses in several plants. However, no studies on U-box E3 ligases have been performed in one of the important staple crops, barley. Results: In this study, we identified 67 putative U-box E3 ligases from the barley genome and expressed sequence tags (ESTs). Similar to Arabidopsis and rice U-box E3 ligases, most of barley U-box E3 ligases possess evolutionary well-conserved domain organizations. Based on the domain compositions and arrangements, the barley U-box proteins were classified into eight different classes. Along with this new classification, we refined the previously reported classifications of U-box E3 ligase genes in Arabidopsis and rice. Furthermore, we investigated the expression profile of 67 U-box E3 ligase genes in response to drought stress and pathogen infection. We observed that many U-box E3 ligase genes were specifically up-and-down regulated by drought stress or by fungal infection, implying their possible roles of some U-box E3 ligase genes in the stress responses. Conclusion: This study reports the classification of U-box E3 ligases in barley and their expression profiles against drought stress and pathogen infection. Therefore, the classification and expression profiling of barley U-box genes can be used as a platform to functionally define the stress-related E3 ligases in barley.
AB - Background: Controlled turnover of proteins as mediated by the ubiquitin proteasome system (UPS) is an important element in plant defense against environmental and pathogen stresses. E3 ligases play a central role in subjecting proteins to hydrolysis by the UPS. Recently, it has been demonstrated that a specific class of E3 ligases termed the U-box ligases are directly associated with the defense mechanisms against abiotic and biotic stresses in several plants. However, no studies on U-box E3 ligases have been performed in one of the important staple crops, barley. Results: In this study, we identified 67 putative U-box E3 ligases from the barley genome and expressed sequence tags (ESTs). Similar to Arabidopsis and rice U-box E3 ligases, most of barley U-box E3 ligases possess evolutionary well-conserved domain organizations. Based on the domain compositions and arrangements, the barley U-box proteins were classified into eight different classes. Along with this new classification, we refined the previously reported classifications of U-box E3 ligase genes in Arabidopsis and rice. Furthermore, we investigated the expression profile of 67 U-box E3 ligase genes in response to drought stress and pathogen infection. We observed that many U-box E3 ligase genes were specifically up-and-down regulated by drought stress or by fungal infection, implying their possible roles of some U-box E3 ligase genes in the stress responses. Conclusion: This study reports the classification of U-box E3 ligases in barley and their expression profiles against drought stress and pathogen infection. Therefore, the classification and expression profiling of barley U-box genes can be used as a platform to functionally define the stress-related E3 ligases in barley.
KW - Abiotic stress
KW - Barley
KW - Biotic stress
KW - Hordeum vulgare
KW - Ubiquitin proteasome system (UPS)
UR - http://www.scopus.com/inward/record.url?scp=85065024074&partnerID=8YFLogxK
U2 - 10.1186/s12864-019-5696-z
DO - 10.1186/s12864-019-5696-z
M3 - Journal article
C2 - 31035917
AN - SCOPUS:85065024074
VL - 20
SP - 1
EP - 15
JO - BMC Genomics
JF - BMC Genomics
SN - 1471-2164
M1 - 326
ER -
ID: 224334304