Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae

Research output: Contribution to journal › Journal article › Research › peer-review

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Rearing zombie flies : Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae. / Edwards, Sam; De Fine Licht, Henrik H.

In: MethodsX, Vol. 12, 102523, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Edwards, S & De Fine Licht, HH 2024, 'Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae', MethodsX, vol. 12, 102523. https://doi.org/10.1016/j.mex.2023.102523

APA

Edwards, S., & De Fine Licht, H. H. (2024). Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae. MethodsX, 12, [102523]. https://doi.org/10.1016/j.mex.2023.102523

Vancouver

Edwards S, De Fine Licht HH. Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae. MethodsX. 2024;12. 102523. https://doi.org/10.1016/j.mex.2023.102523

Author

Edwards, Sam ; De Fine Licht, Henrik H. / Rearing zombie flies : Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae. In: MethodsX. 2024 ; Vol. 12.

Bibtex

@article{44c7b2f81e614ad0aba8ed6d5d8f06cd,
title = "Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae",
abstract = "Insect pathogenic fungi (IPF) and insects have ubiquitous interactions in nature. The extent of these interkingdom host-pathogen interactions are both complex and diverse. Some IPF, notably of the order Entomophthorales, manipulate their species-specific host before death. The fungus-induced altered insect behaviours are sequential and can accurately be repeatedly characterised temporally, making them a valuable model for understanding the molecular and chemical underpinnings of behaviour and host-pathogen co-evolutionary biology. Here, we present methods for the isolation and laboratory culturing of the emerging behaviourally manipulating model IPF Entomophthora muscae for experimentation. • E. muscae isolation and culturing in vitro. • Establishing and maintaining an E. muscae culture in vivo in houseflies (Musca domestica). • Controlled E. muscae infections for virulence experiments and quantification of conidia discharge per cadaver.",
keywords = "Behavioural manipulation model, Diptera, Host-parasite interaction, In vitro, In vivo, Insect pathogenic fungus",
author = "Sam Edwards and {De Fine Licht}, {Henrik H.}",
note = "Publisher Copyright: {\textcopyright} 2023",
year = "2024",
doi = "10.1016/j.mex.2023.102523",
language = "English",
volume = "12",
journal = "MethodsX",
issn = "2215-0161",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Rearing zombie flies

T2 - Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae

AU - Edwards, Sam

AU - De Fine Licht, Henrik H.

N1 - Publisher Copyright: © 2023

PY - 2024

Y1 - 2024

N2 - Insect pathogenic fungi (IPF) and insects have ubiquitous interactions in nature. The extent of these interkingdom host-pathogen interactions are both complex and diverse. Some IPF, notably of the order Entomophthorales, manipulate their species-specific host before death. The fungus-induced altered insect behaviours are sequential and can accurately be repeatedly characterised temporally, making them a valuable model for understanding the molecular and chemical underpinnings of behaviour and host-pathogen co-evolutionary biology. Here, we present methods for the isolation and laboratory culturing of the emerging behaviourally manipulating model IPF Entomophthora muscae for experimentation. • E. muscae isolation and culturing in vitro. • Establishing and maintaining an E. muscae culture in vivo in houseflies (Musca domestica). • Controlled E. muscae infections for virulence experiments and quantification of conidia discharge per cadaver.

AB - Insect pathogenic fungi (IPF) and insects have ubiquitous interactions in nature. The extent of these interkingdom host-pathogen interactions are both complex and diverse. Some IPF, notably of the order Entomophthorales, manipulate their species-specific host before death. The fungus-induced altered insect behaviours are sequential and can accurately be repeatedly characterised temporally, making them a valuable model for understanding the molecular and chemical underpinnings of behaviour and host-pathogen co-evolutionary biology. Here, we present methods for the isolation and laboratory culturing of the emerging behaviourally manipulating model IPF Entomophthora muscae for experimentation. • E. muscae isolation and culturing in vitro. • Establishing and maintaining an E. muscae culture in vivo in houseflies (Musca domestica). • Controlled E. muscae infections for virulence experiments and quantification of conidia discharge per cadaver.

KW - Behavioural manipulation model

KW - Diptera

KW - Host-parasite interaction

KW - In vitro

KW - In vivo

KW - Insect pathogenic fungus

U2 - 10.1016/j.mex.2023.102523

DO - 10.1016/j.mex.2023.102523

M3 - Journal article

C2 - 38179068

AN - SCOPUS:85180121735

VL - 12

JO - MethodsX

JF - MethodsX

SN - 2215-0161

M1 - 102523

ER -

ID: 379794527