How to prove the existence of metabolons?

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

How to prove the existence of metabolons? / Bassard, Jean-Étienne André; Halkier, Barbara Ann.

In: Phytochemistry Reviews, Vol. 17, No. 2, 2018, p. 211-227.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bassard, J-ÉA & Halkier, BA 2018, 'How to prove the existence of metabolons?', Phytochemistry Reviews, vol. 17, no. 2, pp. 211-227. https://doi.org/10.1007/s11101-017-9509-1

APA

Bassard, J-É. A., & Halkier, B. A. (2018). How to prove the existence of metabolons? Phytochemistry Reviews, 17(2), 211-227. https://doi.org/10.1007/s11101-017-9509-1

Vancouver

Bassard J-ÉA, Halkier BA. How to prove the existence of metabolons? Phytochemistry Reviews. 2018;17(2):211-227. https://doi.org/10.1007/s11101-017-9509-1

Author

Bassard, Jean-Étienne André ; Halkier, Barbara Ann. / How to prove the existence of metabolons?. In: Phytochemistry Reviews. 2018 ; Vol. 17, No. 2. pp. 211-227.

Bibtex

@article{05da99e35c5d414db5dc091d9290605c,
title = "How to prove the existence of metabolons?",
abstract = "Sequential enzymes in biosynthetic pathways are organized in metabolons. It is challenging to provide experimental evidence for the existence of metabolons as biosynthetic pathways are composed of highly dynamic protein–protein interactions. Many different methods are being applied, each with strengths and weaknesses. We will present and evaluate several techniques that have been applied in providing evidence for the orchestration of the biosynthetic pathways of cyanogenic glucosides and glucosinolates in metabolons. These evolutionarily related pathways have ER-localized cytochromes P450 that are proposed to function as anchoring site for assembly of the enzymes into metabolons. Additionally, we have included commonly used techniques, even though they have not been used (yet) on these two pathways. In the review, special attention will be given to less-exploited fluorescence-based methods such as FCS and FLIM. Ultimately, understanding the orchestration of biosynthetic pathways may contribute to successful engineering in heterologous hosts.",
keywords = "Fluorescence correlation spectroscopy, Fluorescence lifetime imaging microscopy, Fluorescence-based protein–protein interaction, Yeast-2-hybrid screen",
author = "Bassard, {Jean-{\'E}tienne Andr{\'e}} and Halkier, {Barbara Ann}",
year = "2018",
doi = "10.1007/s11101-017-9509-1",
language = "English",
volume = "17",
pages = "211--227",
journal = "Phytochemistry Reviews",
issn = "1568-7767",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - How to prove the existence of metabolons?

AU - Bassard, Jean-Étienne André

AU - Halkier, Barbara Ann

PY - 2018

Y1 - 2018

N2 - Sequential enzymes in biosynthetic pathways are organized in metabolons. It is challenging to provide experimental evidence for the existence of metabolons as biosynthetic pathways are composed of highly dynamic protein–protein interactions. Many different methods are being applied, each with strengths and weaknesses. We will present and evaluate several techniques that have been applied in providing evidence for the orchestration of the biosynthetic pathways of cyanogenic glucosides and glucosinolates in metabolons. These evolutionarily related pathways have ER-localized cytochromes P450 that are proposed to function as anchoring site for assembly of the enzymes into metabolons. Additionally, we have included commonly used techniques, even though they have not been used (yet) on these two pathways. In the review, special attention will be given to less-exploited fluorescence-based methods such as FCS and FLIM. Ultimately, understanding the orchestration of biosynthetic pathways may contribute to successful engineering in heterologous hosts.

AB - Sequential enzymes in biosynthetic pathways are organized in metabolons. It is challenging to provide experimental evidence for the existence of metabolons as biosynthetic pathways are composed of highly dynamic protein–protein interactions. Many different methods are being applied, each with strengths and weaknesses. We will present and evaluate several techniques that have been applied in providing evidence for the orchestration of the biosynthetic pathways of cyanogenic glucosides and glucosinolates in metabolons. These evolutionarily related pathways have ER-localized cytochromes P450 that are proposed to function as anchoring site for assembly of the enzymes into metabolons. Additionally, we have included commonly used techniques, even though they have not been used (yet) on these two pathways. In the review, special attention will be given to less-exploited fluorescence-based methods such as FCS and FLIM. Ultimately, understanding the orchestration of biosynthetic pathways may contribute to successful engineering in heterologous hosts.

KW - Fluorescence correlation spectroscopy

KW - Fluorescence lifetime imaging microscopy

KW - Fluorescence-based protein–protein interaction

KW - Yeast-2-hybrid screen

U2 - 10.1007/s11101-017-9509-1

DO - 10.1007/s11101-017-9509-1

M3 - Journal article

C2 - 29755303

VL - 17

SP - 211

EP - 227

JO - Phytochemistry Reviews

JF - Phytochemistry Reviews

SN - 1568-7767

IS - 2

ER -

ID: 222748756