TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes. / Aich, Meghali; Ansari, Asgar Hussain; Ding, Li; Iesmantavicius, Vytautas; Paul, Deepanjan; Choudhary, Chunaram; Maiti, Souvik; Buchholz, Frank; Chakraborty, Debojyoti.

In: Cell Reports, Vol. 42, No. 10, 113177, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Aich, M, Ansari, AH, Ding, L, Iesmantavicius, V, Paul, D, Choudhary, C, Maiti, S, Buchholz, F & Chakraborty, D 2023, 'TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes', Cell Reports, vol. 42, no. 10, 113177. https://doi.org/10.1016/j.celrep.2023.113177

APA

Aich, M., Ansari, A. H., Ding, L., Iesmantavicius, V., Paul, D., Choudhary, C., Maiti, S., Buchholz, F., & Chakraborty, D. (2023). TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes. Cell Reports, 42(10), [113177]. https://doi.org/10.1016/j.celrep.2023.113177

Vancouver

Aich M, Ansari AH, Ding L, Iesmantavicius V, Paul D, Choudhary C et al. TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes. Cell Reports. 2023;42(10). 113177. https://doi.org/10.1016/j.celrep.2023.113177

Author

Aich, Meghali ; Ansari, Asgar Hussain ; Ding, Li ; Iesmantavicius, Vytautas ; Paul, Deepanjan ; Choudhary, Chunaram ; Maiti, Souvik ; Buchholz, Frank ; Chakraborty, Debojyoti. / TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes. In: Cell Reports. 2023 ; Vol. 42, No. 10.

Bibtex

@article{c087dd788e9942999e23f0211ca1e707,
title = "TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes",
abstract = "Embryonic stem cells (ESCs) can undergo lineage-specific differentiation, giving rise to different cell types that constitute an organism. Although roles of transcription factors and chromatin modifiers in these cells have been described, how the alternative splicing (AS) machinery regulates their expression has not been sufficiently explored. Here, we show that the long non-coding RNA (lncRNA)-associated protein TOBF1 modulates the AS of transcripts necessary for maintaining stem cell identity in mouse ESCs. Among the genes affected is serine/arginine splicing factor 1 (SRSF1), whose AS leads to global changes in splicing and expression of a large number of downstream genes involved in the maintenance of ESC pluripotency. By overlaying information derived from TOBF1 chromatin occupancy, the distribution of its pluripotency-associated OCT-SOX binding motifs, and transcripts undergoing differential expression and AS upon its knockout, we describe local nuclear territories where these distinct events converge. Collectively, these contribute to the maintenance of mouse ESC identity.",
keywords = "alternative splicing, CP: Stem cell research, CRISPR interference, mESCs, OCT-SOX, Panct1, pluripotency, SRSF1, TOBF1",
author = "Meghali Aich and Ansari, {Asgar Hussain} and Li Ding and Vytautas Iesmantavicius and Deepanjan Paul and Chunaram Choudhary and Souvik Maiti and Frank Buchholz and Debojyoti Chakraborty",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
doi = "10.1016/j.celrep.2023.113177",
language = "English",
volume = "42",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - TOBF1 modulates mouse embryonic stem cell fate through regulating alternative splicing of pluripotency genes

AU - Aich, Meghali

AU - Ansari, Asgar Hussain

AU - Ding, Li

AU - Iesmantavicius, Vytautas

AU - Paul, Deepanjan

AU - Choudhary, Chunaram

AU - Maiti, Souvik

AU - Buchholz, Frank

AU - Chakraborty, Debojyoti

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023

Y1 - 2023

N2 - Embryonic stem cells (ESCs) can undergo lineage-specific differentiation, giving rise to different cell types that constitute an organism. Although roles of transcription factors and chromatin modifiers in these cells have been described, how the alternative splicing (AS) machinery regulates their expression has not been sufficiently explored. Here, we show that the long non-coding RNA (lncRNA)-associated protein TOBF1 modulates the AS of transcripts necessary for maintaining stem cell identity in mouse ESCs. Among the genes affected is serine/arginine splicing factor 1 (SRSF1), whose AS leads to global changes in splicing and expression of a large number of downstream genes involved in the maintenance of ESC pluripotency. By overlaying information derived from TOBF1 chromatin occupancy, the distribution of its pluripotency-associated OCT-SOX binding motifs, and transcripts undergoing differential expression and AS upon its knockout, we describe local nuclear territories where these distinct events converge. Collectively, these contribute to the maintenance of mouse ESC identity.

AB - Embryonic stem cells (ESCs) can undergo lineage-specific differentiation, giving rise to different cell types that constitute an organism. Although roles of transcription factors and chromatin modifiers in these cells have been described, how the alternative splicing (AS) machinery regulates their expression has not been sufficiently explored. Here, we show that the long non-coding RNA (lncRNA)-associated protein TOBF1 modulates the AS of transcripts necessary for maintaining stem cell identity in mouse ESCs. Among the genes affected is serine/arginine splicing factor 1 (SRSF1), whose AS leads to global changes in splicing and expression of a large number of downstream genes involved in the maintenance of ESC pluripotency. By overlaying information derived from TOBF1 chromatin occupancy, the distribution of its pluripotency-associated OCT-SOX binding motifs, and transcripts undergoing differential expression and AS upon its knockout, we describe local nuclear territories where these distinct events converge. Collectively, these contribute to the maintenance of mouse ESC identity.

KW - alternative splicing

KW - CP: Stem cell research

KW - CRISPR interference

KW - mESCs

KW - OCT-SOX

KW - Panct1

KW - pluripotency

KW - SRSF1

KW - TOBF1

U2 - 10.1016/j.celrep.2023.113177

DO - 10.1016/j.celrep.2023.113177

M3 - Journal article

C2 - 37751355

AN - SCOPUS:85171970901

VL - 42

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 10

M1 - 113177

ER -

ID: 369122589