The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development
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- The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development
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Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.
Original language | English |
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Article number | 12119 |
Journal | Nature Communications |
Volume | 7 |
Number of pages | 10 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 2016 |
- Journal Article
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