Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting. / Zhu, Chuanhao; Zhang, Xudong; Xu, Renyuan; Zhong, Yuyue; Li, Silu; Li, Jinyuan; Huang, Chenggang; Wu, Wenhao; Zhai, Mingming; Nurzikhan, Seitkali; Blennow, Andreas; Guo, Dongwei.

In: International Journal of Biological Macromolecules, Vol. 231, 123306, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhu, C, Zhang, X, Xu, R, Zhong, Y, Li, S, Li, J, Huang, C, Wu, W, Zhai, M, Nurzikhan, S, Blennow, A & Guo, D 2023, 'Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting', International Journal of Biological Macromolecules, vol. 231, 123306. https://doi.org/10.1016/j.ijbiomac.2023.123306

APA

Zhu, C., Zhang, X., Xu, R., Zhong, Y., Li, S., Li, J., Huang, C., Wu, W., Zhai, M., Nurzikhan, S., Blennow, A., & Guo, D. (2023). Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting. International Journal of Biological Macromolecules, 231, [123306]. https://doi.org/10.1016/j.ijbiomac.2023.123306

Vancouver

Zhu C, Zhang X, Xu R, Zhong Y, Li S, Li J et al. Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting. International Journal of Biological Macromolecules. 2023;231. 123306. https://doi.org/10.1016/j.ijbiomac.2023.123306

Author

Zhu, Chuanhao ; Zhang, Xudong ; Xu, Renyuan ; Zhong, Yuyue ; Li, Silu ; Li, Jinyuan ; Huang, Chenggang ; Wu, Wenhao ; Zhai, Mingming ; Nurzikhan, Seitkali ; Blennow, Andreas ; Guo, Dongwei. / Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting. In: International Journal of Biological Macromolecules. 2023 ; Vol. 231.

Bibtex

@article{90a5f345452a4e999c2dcc53d10529b7,
title = "Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting",
abstract = "Bivariate flow cytometry (FC) sorting with forward scatter (FSC) and side scatter (SSC) is a recently established novel technique to separate starch granules. However, the forming mechanism of starch FC-dependent popula-tion patterns (i.e. the number of subgroups (NS) and FSC/SSC-dependent distribution patterns) remain partly elusive. For this, the correlation of granular size and multi-scale structure of native starches and FC-dependent population patterns was investigated through employing a wide range of native starches originating from different species involving cereal-, pulse-, and tuber crops. Results showed NS was pertinent with particle size, amylose content (AC), amylopectin chains length distribution, lamellar structure, short-range ordered structure. The distinct NS was determined by impacts of native starch FSC / SSC-dependent distribution patterns. Specif-ically, starch granular size significantly correlated with both FSC and SSC-dependent distribution patterns. The proportion of chains with DP 6-12 was the intra-molecular decisive factor to influence short-range ordered structure, finally leading to FSC-dependent distribution patterns. By contrast, AC was another intra-molecular index to determine SSC-dependent distribution patterns through affecting lamellar structure and short-range ordered structure.",
keywords = "Starch granules, Flow cytometry, Population patterns, Particle size, Structural properties, PHYSICOCHEMICAL PROPERTIES, WHEAT, TRITICALE",
author = "Chuanhao Zhu and Xudong Zhang and Renyuan Xu and Yuyue Zhong and Silu Li and Jinyuan Li and Chenggang Huang and Wenhao Wu and Mingming Zhai and Seitkali Nurzikhan and Andreas Blennow and Dongwei Guo",
year = "2023",
doi = "10.1016/j.ijbiomac.2023.123306",
language = "English",
volume = "231",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting

AU - Zhu, Chuanhao

AU - Zhang, Xudong

AU - Xu, Renyuan

AU - Zhong, Yuyue

AU - Li, Silu

AU - Li, Jinyuan

AU - Huang, Chenggang

AU - Wu, Wenhao

AU - Zhai, Mingming

AU - Nurzikhan, Seitkali

AU - Blennow, Andreas

AU - Guo, Dongwei

PY - 2023

Y1 - 2023

N2 - Bivariate flow cytometry (FC) sorting with forward scatter (FSC) and side scatter (SSC) is a recently established novel technique to separate starch granules. However, the forming mechanism of starch FC-dependent popula-tion patterns (i.e. the number of subgroups (NS) and FSC/SSC-dependent distribution patterns) remain partly elusive. For this, the correlation of granular size and multi-scale structure of native starches and FC-dependent population patterns was investigated through employing a wide range of native starches originating from different species involving cereal-, pulse-, and tuber crops. Results showed NS was pertinent with particle size, amylose content (AC), amylopectin chains length distribution, lamellar structure, short-range ordered structure. The distinct NS was determined by impacts of native starch FSC / SSC-dependent distribution patterns. Specif-ically, starch granular size significantly correlated with both FSC and SSC-dependent distribution patterns. The proportion of chains with DP 6-12 was the intra-molecular decisive factor to influence short-range ordered structure, finally leading to FSC-dependent distribution patterns. By contrast, AC was another intra-molecular index to determine SSC-dependent distribution patterns through affecting lamellar structure and short-range ordered structure.

AB - Bivariate flow cytometry (FC) sorting with forward scatter (FSC) and side scatter (SSC) is a recently established novel technique to separate starch granules. However, the forming mechanism of starch FC-dependent popula-tion patterns (i.e. the number of subgroups (NS) and FSC/SSC-dependent distribution patterns) remain partly elusive. For this, the correlation of granular size and multi-scale structure of native starches and FC-dependent population patterns was investigated through employing a wide range of native starches originating from different species involving cereal-, pulse-, and tuber crops. Results showed NS was pertinent with particle size, amylose content (AC), amylopectin chains length distribution, lamellar structure, short-range ordered structure. The distinct NS was determined by impacts of native starch FSC / SSC-dependent distribution patterns. Specif-ically, starch granular size significantly correlated with both FSC and SSC-dependent distribution patterns. The proportion of chains with DP 6-12 was the intra-molecular decisive factor to influence short-range ordered structure, finally leading to FSC-dependent distribution patterns. By contrast, AC was another intra-molecular index to determine SSC-dependent distribution patterns through affecting lamellar structure and short-range ordered structure.

KW - Starch granules

KW - Flow cytometry

KW - Population patterns

KW - Particle size

KW - Structural properties

KW - PHYSICOCHEMICAL PROPERTIES

KW - WHEAT

KW - TRITICALE

U2 - 10.1016/j.ijbiomac.2023.123306

DO - 10.1016/j.ijbiomac.2023.123306

M3 - Journal article

C2 - 36669629

VL - 231

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

M1 - 123306

ER -

ID: 337585786