Plants have evolved signaling mechanisms that guide growth away from adverse environments that can cause yield losses. Root halotropism is a sodium-specific negative tropism that is crucial for surviving and thriving under high salinity. Although root halotropism was discovered some years ago, the underlying molecular and cellular mechanisms remain unknown. Here, we show that abscisic acid (ABA)-mediated root twisting determines halotropism in Arabidopsis. An ABA-activated SnRK2 protein kinase (SnRK2.6) phosphorylates the microtubule-associated protein SP2L at Ser406, which induces a change in the anisotropic cell expansion at the root transition zone and is required for root twisting during halotropism. Salt stress triggers SP2L-mediated cortical microtubule reorientation, which guides cellulose microfibril patterns. Our findings thus outline the molecular mechanism of root halotropism and indicate that anisotropic cell expansion through microtubule reorientation and microfibril deposition has a central role in mediating tropic responses.