Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures. / Hansen, Sara Fasmer; Ebert, Berit; Rautengarten, Carsten; Heazlewood, Joshua L.

The golgi complex: methods and protocols. ed. / William J. Brown. Springer, 2016. p. 91-109 (Methods in Molecular Biology, Vol. 1496).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Hansen, SF, Ebert, B, Rautengarten, C & Heazlewood, JL 2016, Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures. in WJ Brown (ed.), The golgi complex: methods and protocols. Springer, Methods in Molecular Biology, vol. 1496, pp. 91-109. https://doi.org/10.1007/978-1-4939-6463-5_8

APA

Hansen, S. F., Ebert, B., Rautengarten, C., & Heazlewood, J. L. (2016). Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures. In W. J. Brown (Ed.), The golgi complex: methods and protocols (pp. 91-109). Springer. Methods in Molecular Biology Vol. 1496 https://doi.org/10.1007/978-1-4939-6463-5_8

Vancouver

Hansen SF, Ebert B, Rautengarten C, Heazlewood JL. Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures. In Brown WJ, editor, The golgi complex: methods and protocols. Springer. 2016. p. 91-109. (Methods in Molecular Biology, Vol. 1496). https://doi.org/10.1007/978-1-4939-6463-5_8

Author

Hansen, Sara Fasmer ; Ebert, Berit ; Rautengarten, Carsten ; Heazlewood, Joshua L. / Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures. The golgi complex: methods and protocols. editor / William J. Brown. Springer, 2016. pp. 91-109 (Methods in Molecular Biology, Vol. 1496).

Bibtex

@inbook{0202757b4959460e95790d4fe83ce458,
title = "Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures",
abstract = "The plant Golgi apparatus has a central role in the secretory pathway and is the principal site within the cell for the assembly and processing of macromolecules. The stacked membrane structure of the Golgi apparatus along with its interactions with the cytoskeleton and endoplasmic reticulum has historically made the isolation and purification of this organelle difficult. Density centrifugation has typically been used to enrich Golgi membranes from plant microsomal preparations, and aside from minor adaptations, the approach is still widely employed. Here we outline the enrichment of Golgi membranes from an Arabidopsis cell suspension culture that can be used to investigate the proteome of this organelle. We also provide a useful workflow for the examination of proteomic data as the result of multiple analyses. Finally, we highlight a simple technique to validate the subcellular localization of proteins by fluorescent tags after their identification by tandem mass spectrometry.",
keywords = "Journal Article",
author = "Hansen, {Sara Fasmer} and Berit Ebert and Carsten Rautengarten and Heazlewood, {Joshua L.}",
year = "2016",
doi = "10.1007/978-1-4939-6463-5_8",
language = "English",
isbn = "978-1-4939-6461-1",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "91--109",
editor = "Brown, {William J.}",
booktitle = "The golgi complex",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Proteomic characterization of golgi membranes enriched from Arabidopsis suspension cell cultures

AU - Hansen, Sara Fasmer

AU - Ebert, Berit

AU - Rautengarten, Carsten

AU - Heazlewood, Joshua L.

PY - 2016

Y1 - 2016

N2 - The plant Golgi apparatus has a central role in the secretory pathway and is the principal site within the cell for the assembly and processing of macromolecules. The stacked membrane structure of the Golgi apparatus along with its interactions with the cytoskeleton and endoplasmic reticulum has historically made the isolation and purification of this organelle difficult. Density centrifugation has typically been used to enrich Golgi membranes from plant microsomal preparations, and aside from minor adaptations, the approach is still widely employed. Here we outline the enrichment of Golgi membranes from an Arabidopsis cell suspension culture that can be used to investigate the proteome of this organelle. We also provide a useful workflow for the examination of proteomic data as the result of multiple analyses. Finally, we highlight a simple technique to validate the subcellular localization of proteins by fluorescent tags after their identification by tandem mass spectrometry.

AB - The plant Golgi apparatus has a central role in the secretory pathway and is the principal site within the cell for the assembly and processing of macromolecules. The stacked membrane structure of the Golgi apparatus along with its interactions with the cytoskeleton and endoplasmic reticulum has historically made the isolation and purification of this organelle difficult. Density centrifugation has typically been used to enrich Golgi membranes from plant microsomal preparations, and aside from minor adaptations, the approach is still widely employed. Here we outline the enrichment of Golgi membranes from an Arabidopsis cell suspension culture that can be used to investigate the proteome of this organelle. We also provide a useful workflow for the examination of proteomic data as the result of multiple analyses. Finally, we highlight a simple technique to validate the subcellular localization of proteins by fluorescent tags after their identification by tandem mass spectrometry.

KW - Journal Article

U2 - 10.1007/978-1-4939-6463-5_8

DO - 10.1007/978-1-4939-6463-5_8

M3 - Book chapter

C2 - 27632004

SN - 978-1-4939-6461-1

T3 - Methods in Molecular Biology

SP - 91

EP - 109

BT - The golgi complex

A2 - Brown, William J.

PB - Springer

ER -

ID: 166197177