Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings. / Ruhnow, Felix; Persson, Staffan; Schneider, René.

The Plant Cytoskeleton: Methods and Protocols. Springer, 2023. p. 297-309 (Methods in Molecular Biology, Vol. 2604).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Ruhnow, F, Persson, S & Schneider, R 2023, Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings. in The Plant Cytoskeleton: Methods and Protocols. Springer, Methods in Molecular Biology, vol. 2604, pp. 297-309. https://doi.org/10.1007/978-1-0716-2867-6_24

APA

Ruhnow, F., Persson, S., & Schneider, R. (2023). Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings. In The Plant Cytoskeleton: Methods and Protocols (pp. 297-309). Springer. Methods in Molecular Biology Vol. 2604 https://doi.org/10.1007/978-1-0716-2867-6_24

Vancouver

Ruhnow F, Persson S, Schneider R. Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings. In The Plant Cytoskeleton: Methods and Protocols. Springer. 2023. p. 297-309. (Methods in Molecular Biology, Vol. 2604). https://doi.org/10.1007/978-1-0716-2867-6_24

Author

Ruhnow, Felix ; Persson, Staffan ; Schneider, René. / Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings. The Plant Cytoskeleton: Methods and Protocols. Springer, 2023. pp. 297-309 (Methods in Molecular Biology, Vol. 2604).

Bibtex

@inbook{57dc3fab5c9d4fa5b211648326d6974b,
title = "Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings",
abstract = "The preparation of biological samples, especially for live-cell microscopy, remains a major experimental challenge in the lab despite technological advances. In addition, high-resolution microscopy techniques require higher sample quality and uniformity, which is difficult to ensure during manual preparation while maintaining {"}ideal{"} growth conditions. In this protocol, we provide a way out by growing Arabidopsis thaliana seedlings directly in an imaging chamber, which eliminates invasive sample preparation directly before imaging. This method hinges on the precise placement of seeds into imaging chambers, which can be grown in conventional climate chambers. We detail three methods to grow hypocotyls, cotyledons, leaves, and roots for high-resolution and long-term imaging of the plant cytoskeleton. Furthermore, we show that the growth and development of seedlings inside the chambers can be externally manipulated by the addition of chemicals.",
keywords = "Arabidopsis, Custom-built imaging chambers, Microtubules, Salt stress, Secondary cell walls, Vertical stage",
author = "Felix Ruhnow and Staffan Persson and Ren{\'e} Schneider",
note = "Publisher Copyright: {\textcopyright} 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2867-6_24",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "297--309",
booktitle = "The Plant Cytoskeleton",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings

AU - Ruhnow, Felix

AU - Persson, Staffan

AU - Schneider, René

N1 - Publisher Copyright: © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2023

Y1 - 2023

N2 - The preparation of biological samples, especially for live-cell microscopy, remains a major experimental challenge in the lab despite technological advances. In addition, high-resolution microscopy techniques require higher sample quality and uniformity, which is difficult to ensure during manual preparation while maintaining "ideal" growth conditions. In this protocol, we provide a way out by growing Arabidopsis thaliana seedlings directly in an imaging chamber, which eliminates invasive sample preparation directly before imaging. This method hinges on the precise placement of seeds into imaging chambers, which can be grown in conventional climate chambers. We detail three methods to grow hypocotyls, cotyledons, leaves, and roots for high-resolution and long-term imaging of the plant cytoskeleton. Furthermore, we show that the growth and development of seedlings inside the chambers can be externally manipulated by the addition of chemicals.

AB - The preparation of biological samples, especially for live-cell microscopy, remains a major experimental challenge in the lab despite technological advances. In addition, high-resolution microscopy techniques require higher sample quality and uniformity, which is difficult to ensure during manual preparation while maintaining "ideal" growth conditions. In this protocol, we provide a way out by growing Arabidopsis thaliana seedlings directly in an imaging chamber, which eliminates invasive sample preparation directly before imaging. This method hinges on the precise placement of seeds into imaging chambers, which can be grown in conventional climate chambers. We detail three methods to grow hypocotyls, cotyledons, leaves, and roots for high-resolution and long-term imaging of the plant cytoskeleton. Furthermore, we show that the growth and development of seedlings inside the chambers can be externally manipulated by the addition of chemicals.

KW - Arabidopsis

KW - Custom-built imaging chambers

KW - Microtubules

KW - Salt stress

KW - Secondary cell walls

KW - Vertical stage

U2 - 10.1007/978-1-0716-2867-6_24

DO - 10.1007/978-1-0716-2867-6_24

M3 - Book chapter

C2 - 36773244

AN - SCOPUS:85147894014

T3 - Methods in Molecular Biology

SP - 297

EP - 309

BT - The Plant Cytoskeleton

PB - Springer

ER -

ID: 340790569