Mass spectrometry approaches to study plant endomembrane trafficking

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Mass spectrometry approaches to study plant endomembrane trafficking. / Parsons, Harriet Tempé; Lilley, Kathryn S.

In: Seminars in Cell and Developmental Biology, Vol. 80, 2018, p. 123-132.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Parsons, HT & Lilley, KS 2018, 'Mass spectrometry approaches to study plant endomembrane trafficking', Seminars in Cell and Developmental Biology, vol. 80, pp. 123-132. https://doi.org/10.1016/j.semcdb.2017.10.014

APA

Parsons, H. T., & Lilley, K. S. (2018). Mass spectrometry approaches to study plant endomembrane trafficking. Seminars in Cell and Developmental Biology, 80, 123-132. https://doi.org/10.1016/j.semcdb.2017.10.014

Vancouver

Parsons HT, Lilley KS. Mass spectrometry approaches to study plant endomembrane trafficking. Seminars in Cell and Developmental Biology. 2018;80:123-132. https://doi.org/10.1016/j.semcdb.2017.10.014

Author

Parsons, Harriet Tempé ; Lilley, Kathryn S. / Mass spectrometry approaches to study plant endomembrane trafficking. In: Seminars in Cell and Developmental Biology. 2018 ; Vol. 80. pp. 123-132.

Bibtex

@article{12eb4fc105624e0eb829abd5ea4cf84c,
title = "Mass spectrometry approaches to study plant endomembrane trafficking",
abstract = "Intracellular proteins reside in highly controlled microenvironments in which they perform context specific functions. Trafficking pathways have evolved that enable proteins to be precisely delivered to the correct location but also to re-locate in response to environmental perturbation. Trafficking of membrane proteins to their correct endomembrane location is especially important to enable them to carry out their function. Although a considerable amount of knowledge about membrane protein trafficking in plants has been delivered by years of dedicated research, there are still significant gaps in our understanding of this process. Further knowledge of endomembrane trafficking is dependent on thorough characterization of the subcellular components that constitute the endomembrane system. Such studies are challenging for a number of reasons including the complexity of the plant endomembrane system, inability to purify individual constituents, discrimination protein cargo for full time residents of compartments, and the fact that many proteins function at more than one location. In this review, we describe the components of the secretory pathway and focus on how mass spectrometry based proteomics methods have helped elucidation of this pathway. We demonstrate that the combination of targeted and untargeted approaches is allowing research into new areas of the secretory pathway investigation. Finally we describe new enabling technologies that will impact future studies in this area.",
author = "Parsons, {Harriet Temp{\'e}} and Lilley, {Kathryn S.}",
note = "Copyright {\textcopyright} 2017. Published by Elsevier Ltd.",
year = "2018",
doi = "10.1016/j.semcdb.2017.10.014",
language = "English",
volume = "80",
pages = "123--132",
journal = "Seminars in Cell and Developmental Biology",
issn = "1084-9521",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Mass spectrometry approaches to study plant endomembrane trafficking

AU - Parsons, Harriet Tempé

AU - Lilley, Kathryn S.

N1 - Copyright © 2017. Published by Elsevier Ltd.

PY - 2018

Y1 - 2018

N2 - Intracellular proteins reside in highly controlled microenvironments in which they perform context specific functions. Trafficking pathways have evolved that enable proteins to be precisely delivered to the correct location but also to re-locate in response to environmental perturbation. Trafficking of membrane proteins to their correct endomembrane location is especially important to enable them to carry out their function. Although a considerable amount of knowledge about membrane protein trafficking in plants has been delivered by years of dedicated research, there are still significant gaps in our understanding of this process. Further knowledge of endomembrane trafficking is dependent on thorough characterization of the subcellular components that constitute the endomembrane system. Such studies are challenging for a number of reasons including the complexity of the plant endomembrane system, inability to purify individual constituents, discrimination protein cargo for full time residents of compartments, and the fact that many proteins function at more than one location. In this review, we describe the components of the secretory pathway and focus on how mass spectrometry based proteomics methods have helped elucidation of this pathway. We demonstrate that the combination of targeted and untargeted approaches is allowing research into new areas of the secretory pathway investigation. Finally we describe new enabling technologies that will impact future studies in this area.

AB - Intracellular proteins reside in highly controlled microenvironments in which they perform context specific functions. Trafficking pathways have evolved that enable proteins to be precisely delivered to the correct location but also to re-locate in response to environmental perturbation. Trafficking of membrane proteins to their correct endomembrane location is especially important to enable them to carry out their function. Although a considerable amount of knowledge about membrane protein trafficking in plants has been delivered by years of dedicated research, there are still significant gaps in our understanding of this process. Further knowledge of endomembrane trafficking is dependent on thorough characterization of the subcellular components that constitute the endomembrane system. Such studies are challenging for a number of reasons including the complexity of the plant endomembrane system, inability to purify individual constituents, discrimination protein cargo for full time residents of compartments, and the fact that many proteins function at more than one location. In this review, we describe the components of the secretory pathway and focus on how mass spectrometry based proteomics methods have helped elucidation of this pathway. We demonstrate that the combination of targeted and untargeted approaches is allowing research into new areas of the secretory pathway investigation. Finally we describe new enabling technologies that will impact future studies in this area.

U2 - 10.1016/j.semcdb.2017.10.014

DO - 10.1016/j.semcdb.2017.10.014

M3 - Review

C2 - 29042236

VL - 80

SP - 123

EP - 132

JO - Seminars in Cell and Developmental Biology

JF - Seminars in Cell and Developmental Biology

SN - 1084-9521

ER -

ID: 195464900