Low-coherence quantitative differential phase-contrast microscopy using Talbot interferometry
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Low-coherence quantitative differential phase-contrast microscopy using Talbot interferometry. / Tajbakhsh, Kiarash; Ebrahimi, Samira; Dashtdar, Masoomeh.
In: Applied Optics, Vol. 61, No. 2, 2022, p. 398-402.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Low-coherence quantitative differential phase-contrast microscopy using Talbot interferometry
AU - Tajbakhsh, Kiarash
AU - Ebrahimi, Samira
AU - Dashtdar, Masoomeh
N1 - Publisher Copyright: © 2022 Optica Publishing Group
PY - 2022
Y1 - 2022
N2 - This paper presents a simple, cost-efficient, and highly stable quantitative differential phase-contrast (PC) microscopy based on Talbot interferometry. The proposed system is composed of an optical microscope coupled with a pair of Ronchi amplitude gratings that utilizes a light-emitting diode as a low temporal coherence light source. The quantitative differential PC images of the microscopic transparent samples are reconstructed by analyzing the deformation of moiré patterns using a phase-shifting procedure. Low temporal coherence leads to eliminating speckle noise and undesirable interferences to obtain high-quality images. The spatial phase stability of the system is investigated and compared to two other common-path interferometers. Additionally, the performance of the method is verified by the experimental results of a standard resolution test target and phase biological samples.
AB - This paper presents a simple, cost-efficient, and highly stable quantitative differential phase-contrast (PC) microscopy based on Talbot interferometry. The proposed system is composed of an optical microscope coupled with a pair of Ronchi amplitude gratings that utilizes a light-emitting diode as a low temporal coherence light source. The quantitative differential PC images of the microscopic transparent samples are reconstructed by analyzing the deformation of moiré patterns using a phase-shifting procedure. Low temporal coherence leads to eliminating speckle noise and undesirable interferences to obtain high-quality images. The spatial phase stability of the system is investigated and compared to two other common-path interferometers. Additionally, the performance of the method is verified by the experimental results of a standard resolution test target and phase biological samples.
U2 - 10.1364/AO.445369
DO - 10.1364/AO.445369
M3 - Journal article
C2 - 35200875
AN - SCOPUS:85122293112
VL - 61
SP - 398
EP - 402
JO - Applied Optics
JF - Applied Optics
SN - 1559-128X
IS - 2
ER -
ID: 289962040