GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements. / Huang, Junfeng; Chen, Feng; Guo, Yanjun; Gan, Xinli; Yang, Mingming; Zeng, Wei; Persson, Staffan; Li, Juan; Xu, Wenliang.

In: New Phytologist, Vol. 232, No. 4, 2021, p. 1718-1737.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Huang, J, Chen, F, Guo, Y, Gan, X, Yang, M, Zeng, W, Persson, S, Li, J & Xu, W 2021, 'GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements', New Phytologist, vol. 232, no. 4, pp. 1718-1737. https://doi.org/10.1111/nph.17612

APA

Huang, J., Chen, F., Guo, Y., Gan, X., Yang, M., Zeng, W., Persson, S., Li, J., & Xu, W. (2021). GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements. New Phytologist, 232(4), 1718-1737. https://doi.org/10.1111/nph.17612

Vancouver

Huang J, Chen F, Guo Y, Gan X, Yang M, Zeng W et al. GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements. New Phytologist. 2021;232(4):1718-1737. https://doi.org/10.1111/nph.17612

Author

Huang, Junfeng ; Chen, Feng ; Guo, Yanjun ; Gan, Xinli ; Yang, Mingming ; Zeng, Wei ; Persson, Staffan ; Li, Juan ; Xu, Wenliang. / GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements. In: New Phytologist. 2021 ; Vol. 232, No. 4. pp. 1718-1737.

Bibtex

@article{f2b7db42ddc94f98ab17564338ea0ade,
title = "GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements",
abstract = "Cotton fibre is the most important source for natural textiles. The secondary cell walls (SCWs) of mature cotton fibres contain the highest proportion of cellulose content (> 90%) in any plant. The onset and progression of SCW cellulose synthesis need to be tightly controlled to balance fibre elongation and cell wall deposition. However, regulatory mechanisms that control cellulose synthesis during cotton fibre growth remain elusive. Here, we conducted genetic and functional analyses demonstrating that the R2R3-MYB GhMYB7 controls cotton fibre cellulose synthesis. Overexpression of GhMYB7 in cotton sped up SCW cellulose biosynthesis in fibre cells, and led to shorter fibres with thicker walls. By contrast, RNA interference (RNAi) silencing of GhMYB7 delayed fibre SCW cellulose synthesis and resulted in elongated fibres with thinner walls. Furthermore, we demonstrated that GhMYB7 regulated cotton fibre SCW cellulose synthases by directly binding to three distinct cis-elements in the respective GhCesA4, GhCesA7 and GhCesA8 promoters. We found that this regulatory mechanism of cellulose synthesis was 'hi-jacked' also by other GhMYBs. Together, our findings uncover a hitherto-unknown mechanism that cotton fibre employs to regulate SCW cellulose synthesis. Our results also provide a strategy for genetic improvement of SCW thickness of cotton fibre.",
keywords = "cellulose synthesis, cis-elements, cotton fibre, MYB, secondary cell wall, TRANSCRIPTION FACTOR, CELL ELONGATION, BIOSYNTHESIS, ARABIDOPSIS, IDENTIFICATION, PROTEINS, SEQUENCE, XYLEM, NAC",
author = "Junfeng Huang and Feng Chen and Yanjun Guo and Xinli Gan and Mingming Yang and Wei Zeng and Staffan Persson and Juan Li and Wenliang Xu",
year = "2021",
doi = "10.1111/nph.17612",
language = "English",
volume = "232",
pages = "1718--1737",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Academic Press",
number = "4",

}

RIS

TY - JOUR

T1 - GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements

AU - Huang, Junfeng

AU - Chen, Feng

AU - Guo, Yanjun

AU - Gan, Xinli

AU - Yang, Mingming

AU - Zeng, Wei

AU - Persson, Staffan

AU - Li, Juan

AU - Xu, Wenliang

PY - 2021

Y1 - 2021

N2 - Cotton fibre is the most important source for natural textiles. The secondary cell walls (SCWs) of mature cotton fibres contain the highest proportion of cellulose content (> 90%) in any plant. The onset and progression of SCW cellulose synthesis need to be tightly controlled to balance fibre elongation and cell wall deposition. However, regulatory mechanisms that control cellulose synthesis during cotton fibre growth remain elusive. Here, we conducted genetic and functional analyses demonstrating that the R2R3-MYB GhMYB7 controls cotton fibre cellulose synthesis. Overexpression of GhMYB7 in cotton sped up SCW cellulose biosynthesis in fibre cells, and led to shorter fibres with thicker walls. By contrast, RNA interference (RNAi) silencing of GhMYB7 delayed fibre SCW cellulose synthesis and resulted in elongated fibres with thinner walls. Furthermore, we demonstrated that GhMYB7 regulated cotton fibre SCW cellulose synthases by directly binding to three distinct cis-elements in the respective GhCesA4, GhCesA7 and GhCesA8 promoters. We found that this regulatory mechanism of cellulose synthesis was 'hi-jacked' also by other GhMYBs. Together, our findings uncover a hitherto-unknown mechanism that cotton fibre employs to regulate SCW cellulose synthesis. Our results also provide a strategy for genetic improvement of SCW thickness of cotton fibre.

AB - Cotton fibre is the most important source for natural textiles. The secondary cell walls (SCWs) of mature cotton fibres contain the highest proportion of cellulose content (> 90%) in any plant. The onset and progression of SCW cellulose synthesis need to be tightly controlled to balance fibre elongation and cell wall deposition. However, regulatory mechanisms that control cellulose synthesis during cotton fibre growth remain elusive. Here, we conducted genetic and functional analyses demonstrating that the R2R3-MYB GhMYB7 controls cotton fibre cellulose synthesis. Overexpression of GhMYB7 in cotton sped up SCW cellulose biosynthesis in fibre cells, and led to shorter fibres with thicker walls. By contrast, RNA interference (RNAi) silencing of GhMYB7 delayed fibre SCW cellulose synthesis and resulted in elongated fibres with thinner walls. Furthermore, we demonstrated that GhMYB7 regulated cotton fibre SCW cellulose synthases by directly binding to three distinct cis-elements in the respective GhCesA4, GhCesA7 and GhCesA8 promoters. We found that this regulatory mechanism of cellulose synthesis was 'hi-jacked' also by other GhMYBs. Together, our findings uncover a hitherto-unknown mechanism that cotton fibre employs to regulate SCW cellulose synthesis. Our results also provide a strategy for genetic improvement of SCW thickness of cotton fibre.

KW - cellulose synthesis

KW - cis-elements

KW - cotton fibre

KW - MYB

KW - secondary cell wall

KW - TRANSCRIPTION FACTOR

KW - CELL ELONGATION

KW - BIOSYNTHESIS

KW - ARABIDOPSIS

KW - IDENTIFICATION

KW - PROTEINS

KW - SEQUENCE

KW - XYLEM

KW - NAC

U2 - 10.1111/nph.17612

DO - 10.1111/nph.17612

M3 - Journal article

C2 - 34245570

VL - 232

SP - 1718

EP - 1737

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 4

ER -

ID: 275882301