Fluorescent cytoskeletal markers reveal associations between the actin and microtubule cytoskeleton in rice cells
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Fluorescent cytoskeletal markers reveal associations between the actin and microtubule cytoskeleton in rice cells. / Liu, Zengyu; Østerlund, Isabella; Ruhnow, Felix; Cao, Yiran; Huang, Guoqiang; Cai, Wenguo; Zhang, Jiao; Liang, Wanqi; Nikoloski, Zoran; Persson, Staffan; Zhang, Dabing.
In: Development, Vol. 149, No. 12, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Fluorescent cytoskeletal markers reveal associations between the actin and microtubule cytoskeleton in rice cells
AU - Liu, Zengyu
AU - Østerlund, Isabella
AU - Ruhnow, Felix
AU - Cao, Yiran
AU - Huang, Guoqiang
AU - Cai, Wenguo
AU - Zhang, Jiao
AU - Liang, Wanqi
AU - Nikoloski, Zoran
AU - Persson, Staffan
AU - Zhang, Dabing
N1 - Publisher Copyright: © 2022. Published by The Company of Biologists Ltd.
PY - 2022
Y1 - 2022
N2 - Rice (Oryza sativa) is one of our main food crops, feeding ∼3.5 billion people worldwide. An increasing number of studies note the importance of the cytoskeleton, including actin filaments and microtubules, in rice development and environmental responses. Yet, reliable in vivo cytoskeleton markers are lacking in rice, which limits our knowledge of cytoskeletal functions in living cells. Therefore, we generated bright fluorescent marker lines of the actin and microtubule cytoskeletons in rice, suitable for live-cell imaging in a wide variety of rice tissues. Using these lines, we show that actin bundles and microtubules engage and co-function during pollen grain development, how the cytoskeletal components are coordinated during root cell development, and that the actin cytoskeleton is robust and facilitates microtubule responses during salt stress. Hence, we conclude that our cytoskeletal marker lines, highlighted by our findings of cytoskeletal associations and dynamics, will substantially further future investigations in rice biology.
AB - Rice (Oryza sativa) is one of our main food crops, feeding ∼3.5 billion people worldwide. An increasing number of studies note the importance of the cytoskeleton, including actin filaments and microtubules, in rice development and environmental responses. Yet, reliable in vivo cytoskeleton markers are lacking in rice, which limits our knowledge of cytoskeletal functions in living cells. Therefore, we generated bright fluorescent marker lines of the actin and microtubule cytoskeletons in rice, suitable for live-cell imaging in a wide variety of rice tissues. Using these lines, we show that actin bundles and microtubules engage and co-function during pollen grain development, how the cytoskeletal components are coordinated during root cell development, and that the actin cytoskeleton is robust and facilitates microtubule responses during salt stress. Hence, we conclude that our cytoskeletal marker lines, highlighted by our findings of cytoskeletal associations and dynamics, will substantially further future investigations in rice biology.
KW - Actin
KW - Cytoskeleton
KW - Fluorescent markers
KW - Live-cell imaging
KW - Microtubule
KW - Rice
U2 - 10.1242/dev.200415
DO - 10.1242/dev.200415
M3 - Journal article
C2 - 35587127
AN - SCOPUS:85132456077
VL - 149
JO - Development
JF - Development
SN - 0950-1991
IS - 12
ER -
ID: 315175565