A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum

Research output: Contribution to journal › Journal article › Research › peer-review

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A Walk on the Wild Side : Genome Editing of Tuber-Bearing Solanum bulbocastanum. / Azariadis, Aristotelis; Andrzejczak, Olga A.; Carlsen, Frida M.; Westberg, Ida; Brinch-Pedersen, Henrik; Petersen, Bent L.; Hebelstrup, Kim H.

In: Plants, Vol. 13, No. 7, 1044, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Azariadis, A, Andrzejczak, OA, Carlsen, FM, Westberg, I, Brinch-Pedersen, H, Petersen, BL & Hebelstrup, KH 2024, 'A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum', Plants, vol. 13, no. 7, 1044. https://doi.org/10.3390/plants13071044

APA

Azariadis, A., Andrzejczak, O. A., Carlsen, F. M., Westberg, I., Brinch-Pedersen, H., Petersen, B. L., & Hebelstrup, K. H. (2024). A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum. Plants, 13(7), [1044]. https://doi.org/10.3390/plants13071044

Vancouver

Azariadis A, Andrzejczak OA, Carlsen FM, Westberg I, Brinch-Pedersen H, Petersen BL et al. A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum. Plants. 2024;13(7). 1044. https://doi.org/10.3390/plants13071044

Author

Azariadis, Aristotelis ; Andrzejczak, Olga A. ; Carlsen, Frida M. ; Westberg, Ida ; Brinch-Pedersen, Henrik ; Petersen, Bent L. ; Hebelstrup, Kim H. / A Walk on the Wild Side : Genome Editing of Tuber-Bearing Solanum bulbocastanum. In: Plants. 2024 ; Vol. 13, No. 7.

Bibtex

@article{5a70ef5760024bcdada8287c89cec0de,
title = "A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum",
abstract = "Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. bulbocastanum protoplasts and regeneration of gene-edited plants. We use ribonucleoproteins, consisting of Cas9 and sgRNA, assembled in vitro, to target a gene belonging to the nitrate and peptide transporter family. Four different sgRNAs were designed and we observed efficiency in gene-editing in the protoplast pool between 8.5% and 12.4%. Twenty-one plants were re-generated from microcalli developed from individual protoplasts. In three of the plants we found that the target gene had been edited. Two of the edited plants had deletion mutations introduced into both alleles, whereas one only had a mutation in one of the alleles. Our work demonstrates that protocols for the transformation of Solanum tuberosum can be optimized to be applied to a wild Solanum species.",
keywords = "de novo domestication, genome editing, protoplasts, transgene free, wild potato",
author = "Aristotelis Azariadis and Andrzejczak, {Olga A.} and Carlsen, {Frida M.} and Ida Westberg and Henrik Brinch-Pedersen and Petersen, {Bent L.} and Hebelstrup, {Kim H.}",
note = "Publisher Copyright: {\textcopyright} 2024 by the authors.",
year = "2024",
doi = "10.3390/plants13071044",
language = "English",
volume = "13",
journal = "Plants",
issn = "2223-7747",
publisher = "MDPI AG",
number = "7",

}

RIS

TY - JOUR

T1 - A Walk on the Wild Side

T2 - Genome Editing of Tuber-Bearing Solanum bulbocastanum

AU - Azariadis, Aristotelis

AU - Andrzejczak, Olga A.

AU - Carlsen, Frida M.

AU - Westberg, Ida

AU - Brinch-Pedersen, Henrik

AU - Petersen, Bent L.

AU - Hebelstrup, Kim H.

N1 - Publisher Copyright: © 2024 by the authors.

PY - 2024

Y1 - 2024

N2 - Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. bulbocastanum protoplasts and regeneration of gene-edited plants. We use ribonucleoproteins, consisting of Cas9 and sgRNA, assembled in vitro, to target a gene belonging to the nitrate and peptide transporter family. Four different sgRNAs were designed and we observed efficiency in gene-editing in the protoplast pool between 8.5% and 12.4%. Twenty-one plants were re-generated from microcalli developed from individual protoplasts. In three of the plants we found that the target gene had been edited. Two of the edited plants had deletion mutations introduced into both alleles, whereas one only had a mutation in one of the alleles. Our work demonstrates that protocols for the transformation of Solanum tuberosum can be optimized to be applied to a wild Solanum species.

AB - Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. bulbocastanum protoplasts and regeneration of gene-edited plants. We use ribonucleoproteins, consisting of Cas9 and sgRNA, assembled in vitro, to target a gene belonging to the nitrate and peptide transporter family. Four different sgRNAs were designed and we observed efficiency in gene-editing in the protoplast pool between 8.5% and 12.4%. Twenty-one plants were re-generated from microcalli developed from individual protoplasts. In three of the plants we found that the target gene had been edited. Two of the edited plants had deletion mutations introduced into both alleles, whereas one only had a mutation in one of the alleles. Our work demonstrates that protocols for the transformation of Solanum tuberosum can be optimized to be applied to a wild Solanum species.

KW - de novo domestication

KW - genome editing

KW - protoplasts

KW - transgene free

KW - wild potato

U2 - 10.3390/plants13071044

DO - 10.3390/plants13071044

M3 - Journal article

C2 - 38611572

AN - SCOPUS:85190124991

VL - 13

JO - Plants

JF - Plants

SN - 2223-7747

IS - 7

M1 - 1044

ER -

ID: 389089401