TY - JOUR

T1 - 3D bioprinted pectin-based hydrogel as sustainable biomaterials for musculoskeletal tissue engineering

AU - Chang, Hao Chen

AU - Jørgensen, Bodil

AU - Di Silvio, Lucy

AU - Gurzawska-Comis, Kasia

N1 - Publisher Copyright: © 2023

PY - 2023

Y1 - 2023

N2 - Development of sustainable biomaterials are rapidly emerging as alternatives to fossil-based products. The new generation of eco-friendly “green” biomaterials manufactured using the latest advanced technologies in tissue engineering offers new possibilities for biomedical field in terms of drug delivery, therapeutics and damaged tissues repair. Musculoskeletal tissue has limited self-repair capacity, hence, there is an urgent need for effective treatment modalities to repair and prevent progression to chronic disease. More recently, cellular scaffolds are being considered as new therapeutic approaches for tissue engineering and 3D bioprinting tissues. Despite the success of using bioprinting techniques to create hierarchical constructs with uniformly dispersed cells, there is limited research on “green” bioactive components which can stimulate both osteogenesis and chondrogenesis simultaneously. Based on previous studies; pectin RG-I has been shown to have osteogenic and anti-inflammatory properties. This study focused on the fabrication of a pectin RG-I bioink for the incorporation of chondrocytes. The RG-I was extracted from agricultural side streams from the potato starch. Three different concentrations (0 μg/mL, 500 μg/mL, 700 μg/mL) of RG-I were evaluated. Human chondrocytes were incorporated within the bioinks and 3D bioprinted to create viable scaffolds. Physical properties were characterized by swelling and degradation studies. Cell response was assessed with proliferation, cell viability and morphology, and gene expression of galectin-3, collagen type-l, and collagen type- ll. All three groups of bioinks showed water intake and degradation ability as well as ideal printability and shape fidelity. Bioprinting did not cause any detrimental effect on cell-laden ink and cells were observed to be homogeneously dispersed. The formulation of 500 μg/mL pectin RG-I bioink showed the most promising properties for potential use to repair osteochondral defects due to its anti-inflammatory effect and fibrosis inhibition. The use of RG-I opens possibilities to manufacture multifunctional sustainable “green” biomaterials.

AB - Development of sustainable biomaterials are rapidly emerging as alternatives to fossil-based products. The new generation of eco-friendly “green” biomaterials manufactured using the latest advanced technologies in tissue engineering offers new possibilities for biomedical field in terms of drug delivery, therapeutics and damaged tissues repair. Musculoskeletal tissue has limited self-repair capacity, hence, there is an urgent need for effective treatment modalities to repair and prevent progression to chronic disease. More recently, cellular scaffolds are being considered as new therapeutic approaches for tissue engineering and 3D bioprinting tissues. Despite the success of using bioprinting techniques to create hierarchical constructs with uniformly dispersed cells, there is limited research on “green” bioactive components which can stimulate both osteogenesis and chondrogenesis simultaneously. Based on previous studies; pectin RG-I has been shown to have osteogenic and anti-inflammatory properties. This study focused on the fabrication of a pectin RG-I bioink for the incorporation of chondrocytes. The RG-I was extracted from agricultural side streams from the potato starch. Three different concentrations (0 μg/mL, 500 μg/mL, 700 μg/mL) of RG-I were evaluated. Human chondrocytes were incorporated within the bioinks and 3D bioprinted to create viable scaffolds. Physical properties were characterized by swelling and degradation studies. Cell response was assessed with proliferation, cell viability and morphology, and gene expression of galectin-3, collagen type-l, and collagen type- ll. All three groups of bioinks showed water intake and degradation ability as well as ideal printability and shape fidelity. Bioprinting did not cause any detrimental effect on cell-laden ink and cells were observed to be homogeneously dispersed. The formulation of 500 μg/mL pectin RG-I bioink showed the most promising properties for potential use to repair osteochondral defects due to its anti-inflammatory effect and fibrosis inhibition. The use of RG-I opens possibilities to manufacture multifunctional sustainable “green” biomaterials.

KW - 3D bioprinting

KW - Hydrogel

KW - Osteochondral regeneration

KW - Pectin

KW - Sustainable biomaterials

U2 - 10.1016/j.susmat.2023.e00732

DO - 10.1016/j.susmat.2023.e00732

M3 - Journal article

AN - SCOPUS:85173586962

VL - 38

JO - Sustainable Materials and Technologies

JF - Sustainable Materials and Technologies

SN - 2214-9937

M1 - e00732

ER -