Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls
Research output: Contribution to journal › Journal article › Research › peer-review
To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.
Original language | English |
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Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 103 |
Issue number | 30 |
Pages (from-to) | 11417-11422 |
Number of pages | 6 |
ISSN | 0027-8424 |
DOIs | |
Publication status | Published - 2006 |
Externally published | Yes |
- Arabidopsis, Fingerprinting, Hydrolase, Mutant, Xylan
Research areas
ID: 273641626