Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls. / Bauer, Stefan; Vasu, Prasanna; Persson, Staffan; Mort, Andrew J.; Somerville, Chris R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 30, 2006, p. 11417-11422.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bauer, S, Vasu, P, Persson, S, Mort, AJ & Somerville, CR 2006, 'Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 30, pp. 11417-11422. https://doi.org/10.1073/pnas.0604632103

APA

Bauer, S., Vasu, P., Persson, S., Mort, A. J., & Somerville, C. R. (2006). Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls. Proceedings of the National Academy of Sciences of the United States of America, 103(30), 11417-11422. https://doi.org/10.1073/pnas.0604632103

Vancouver

Bauer S, Vasu P, Persson S, Mort AJ, Somerville CR. Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls. Proceedings of the National Academy of Sciences of the United States of America. 2006;103(30):11417-11422. https://doi.org/10.1073/pnas.0604632103

Author

Bauer, Stefan ; Vasu, Prasanna ; Persson, Staffan ; Mort, Andrew J. ; Somerville, Chris R. / Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 30. pp. 11417-11422.

Bibtex

@article{49e87586d0194777ae12a836ba0e9d4f,
title = "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls",
abstract = "To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.",
keywords = "Arabidopsis, Fingerprinting, Hydrolase, Mutant, Xylan",
author = "Stefan Bauer and Prasanna Vasu and Staffan Persson and Mort, {Andrew J.} and Somerville, {Chris R.}",
year = "2006",
doi = "10.1073/pnas.0604632103",
language = "English",
volume = "103",
pages = "11417--11422",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "30",

}

RIS

TY - JOUR

T1 - Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls

AU - Bauer, Stefan

AU - Vasu, Prasanna

AU - Persson, Staffan

AU - Mort, Andrew J.

AU - Somerville, Chris R.

PY - 2006

Y1 - 2006

N2 - To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.

AB - To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.

KW - Arabidopsis

KW - Fingerprinting

KW - Hydrolase

KW - Mutant

KW - Xylan

U2 - 10.1073/pnas.0604632103

DO - 10.1073/pnas.0604632103

M3 - Journal article

AN - SCOPUS:33746614259

VL - 103

SP - 11417

EP - 11422

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 30

ER -

ID: 273641626