Balanced callose and cellulose biosynthesis in Arabidopsis quorum sensing and pattern-triggered immunity
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Balanced callose and cellulose biosynthesis in Arabidopsis quorum sensing and pattern-triggered immunity. / Liu, Xiaolin; Ma, Zhiming; Tran, Tuan Minh; Rautengarten, Carsten; Cheng, Yingying; Yang, Liang; Ebert, Berit; Persson, Staffan; Miao, Yansong.
In: Plant Physiology, Vol. 194, No. 1, 2024, p. 137-152.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Balanced callose and cellulose biosynthesis in Arabidopsis quorum sensing and pattern-triggered immunity
AU - Liu, Xiaolin
AU - Ma, Zhiming
AU - Tran, Tuan Minh
AU - Rautengarten, Carsten
AU - Cheng, Yingying
AU - Yang, Liang
AU - Ebert, Berit
AU - Persson, Staffan
AU - Miao, Yansong
N1 - © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.
PY - 2024
Y1 - 2024
N2 - The plant cell wall (CW) is one of the most important physical barriers phytopathogens must conquer to invade their hosts. This barrier is a dynamic structure that responds to pathogen infection through a complex network of immune receptors, together with CW-synthesizing and CW-degrading enzymes. Callose deposition in the primary CW is a well-known physical response to pathogen infection. Notably, callose and cellulose biosynthesis share an initial substrate, UDP-glucose, which is the main load-bearing component of the CW. However, how these two critical biosynthetic processes are balanced during plant-pathogen interactions remains unclear. Here, using two different pathogen-derived molecules, bacterial flagellin (flg22) and the diffusible signal factor (DSF) produced by Xanthomonas campestris pv. campestris, we show a negative correlation between cellulose and callose biosynthesis in Arabidopsis (Arabidopsis thaliana). By quantifying the abundance of callose and cellulose under DSF or flg22 elicitation and characterizing the dynamics of the enzymes involved in the biosynthesis and degradation of these two polymers, we show that the balance of these two CW components is mediated by the activity of a β-1,3-glucanase (BG2). Our data demonstrate balanced cellulose and callose biosynthesis during plant immune responses.
AB - The plant cell wall (CW) is one of the most important physical barriers phytopathogens must conquer to invade their hosts. This barrier is a dynamic structure that responds to pathogen infection through a complex network of immune receptors, together with CW-synthesizing and CW-degrading enzymes. Callose deposition in the primary CW is a well-known physical response to pathogen infection. Notably, callose and cellulose biosynthesis share an initial substrate, UDP-glucose, which is the main load-bearing component of the CW. However, how these two critical biosynthetic processes are balanced during plant-pathogen interactions remains unclear. Here, using two different pathogen-derived molecules, bacterial flagellin (flg22) and the diffusible signal factor (DSF) produced by Xanthomonas campestris pv. campestris, we show a negative correlation between cellulose and callose biosynthesis in Arabidopsis (Arabidopsis thaliana). By quantifying the abundance of callose and cellulose under DSF or flg22 elicitation and characterizing the dynamics of the enzymes involved in the biosynthesis and degradation of these two polymers, we show that the balance of these two CW components is mediated by the activity of a β-1,3-glucanase (BG2). Our data demonstrate balanced cellulose and callose biosynthesis during plant immune responses.
U2 - 10.1093/plphys/kiad473
DO - 10.1093/plphys/kiad473
M3 - Journal article
C2 - 37647538
VL - 194
SP - 137
EP - 152
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 1
ER -
ID: 371657855