Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation
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- Transcriptional read-through of the long noncoding RNA SVALKA governs plant cold acclimation
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Most DNA in the genomes of higher organisms does not encode proteins, yet much is transcribed by RNA polymerase II (RNAPII) into long non-coding RNAs (lncRNAs). The biological significance of most lncRNAs is largely unclear. Here, we identify a lncRNA (SVALKA) in a cold-sensitive region of the Arabidopsis genome. Mutations in SVALKA affect CBF1 expression and freezing tolerance. RNAPII read-through transcription of SVALKA results in a cryptic lncRNA overlapping CBF1 on the antisense strand, termed asCBF1. Our molecular dissection reveals that CBF1 is suppressed by RNAPII collision stemming from the SVALKA-asCBF1 lncRNA cascade. The SVALKA-asCBF1 cascade provides a mechanism to tightly control CBF1 expression and timing that could be exploited to maximize freezing tolerance with mitigated fitness costs. Our results provide a compelling example of local gene regulation by lncRNA transcription having a profound impact on the ability of plants to appropriately acclimate to challenging environmental conditions.
Original language | English |
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Article number | 4561 |
Journal | Nature Communications |
Volume | 9 |
Pages (from-to) | 1-11 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 2018 |
Bibliographical note
Author Correction: https://www.nature.com/articles/s41467-019-13269-0
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