Impacts of dietary copper on the swine gut microbiome and antibiotic resistome

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Impacts of dietary copper on the swine gut microbiome and antibiotic resistome. / Brinck, Julius Emil; Lassen, Simon Bo; Forouzandeh, Asal; Pan, Ting; Wang, Yan Zi; Monteiro, Alessandra; Blavi, Laia; Solà-Oriol, David; Stein, Hans H.; Su, Jian Qiang; Brandt, Kristian K.

In: Science of the Total Environment, Vol. 857, 159609, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Brinck, JE, Lassen, SB, Forouzandeh, A, Pan, T, Wang, YZ, Monteiro, A, Blavi, L, Solà-Oriol, D, Stein, HH, Su, JQ & Brandt, KK 2023, 'Impacts of dietary copper on the swine gut microbiome and antibiotic resistome', Science of the Total Environment, vol. 857, 159609. https://doi.org/10.1016/j.scitotenv.2022.159609

APA

Brinck, J. E., Lassen, S. B., Forouzandeh, A., Pan, T., Wang, Y. Z., Monteiro, A., Blavi, L., Solà-Oriol, D., Stein, H. H., Su, J. Q., & Brandt, K. K. (2023). Impacts of dietary copper on the swine gut microbiome and antibiotic resistome. Science of the Total Environment, 857, [159609]. https://doi.org/10.1016/j.scitotenv.2022.159609

Vancouver

Brinck JE, Lassen SB, Forouzandeh A, Pan T, Wang YZ, Monteiro A et al. Impacts of dietary copper on the swine gut microbiome and antibiotic resistome. Science of the Total Environment. 2023;857. 159609. https://doi.org/10.1016/j.scitotenv.2022.159609

Author

Brinck, Julius Emil ; Lassen, Simon Bo ; Forouzandeh, Asal ; Pan, Ting ; Wang, Yan Zi ; Monteiro, Alessandra ; Blavi, Laia ; Solà-Oriol, David ; Stein, Hans H. ; Su, Jian Qiang ; Brandt, Kristian K. / Impacts of dietary copper on the swine gut microbiome and antibiotic resistome. In: Science of the Total Environment. 2023 ; Vol. 857.

Bibtex

@article{1e164cbb8e36446f8f55da6968cfa851,
title = "Impacts of dietary copper on the swine gut microbiome and antibiotic resistome",
abstract = "Restrictions on antibiotic growth promoters have prompted livestock producers to use alternative growth promoters, and dietary copper (Cu) supplementation is currently being widely used in pig production. However, elevated doses of dietary Cu constitute a risk for co-selection of antibiotic resistance and the risk may depend on the type of Cu-based feed additives being used. We here report the first controlled experiment investigating the impact of two contrasting Cu-based feed additives on the overall swine gut microbiome and antibiotic resistome. DNA was extracted from fecal samples (n = 96) collected at four time points during 116 days from 120 pigs allotted to three dietary treatments: control, divalent copper sulfate (CuSO4; 250 μg Cu g−1 feed), and monovalent copper oxide (Cu2O; 250 μg Cu g−1 feed). Bacterial community composition, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) were assessed, and bioavailable Cu ([Cu]bio) was determined using whole-cell bacterial bioreporters. Cu supplementation to feed increased total Cu concentrations ([Cu]total) and [Cu]bio in feces 8–10 fold and at least 670–1000 fold, respectively, but with no significant differences between the two Cu sources. The swine gut microbiome harbored highly abundant and diverse ARGs and MGEs irrespective of the treatments throughout the experiment. Microbiomes differed significantly between pig growth stages and tended to converge over time, but only minor changes in the bacterial community composition and resistome could be linked to Cu supplementation. A significant correlation between bacterial community composition (i.e., bacterial taxa present) and ARG prevalence patterns were observed by Procrustes analysis. Overall, results of the experiment did not provide evidence for Cu-induced co-selection of ARGs or MGEs even at a Cu concentration level exceeding the maximal permitted level for pig diets in the EU (25 to 150 μg Cu g−1 feed depending on pig age).",
keywords = "Antibiotic resistance genes, Antimicrobial resistance, Bacterial community composition, Co-selection, HT-qPCR, Pig microbiome",
author = "Brinck, {Julius Emil} and Lassen, {Simon Bo} and Asal Forouzandeh and Ting Pan and Wang, {Yan Zi} and Alessandra Monteiro and Laia Blavi and David Sol{\`a}-Oriol and Stein, {Hans H.} and Su, {Jian Qiang} and Brandt, {Kristian K.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2023",
doi = "10.1016/j.scitotenv.2022.159609",
language = "English",
volume = "857",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Impacts of dietary copper on the swine gut microbiome and antibiotic resistome

AU - Brinck, Julius Emil

AU - Lassen, Simon Bo

AU - Forouzandeh, Asal

AU - Pan, Ting

AU - Wang, Yan Zi

AU - Monteiro, Alessandra

AU - Blavi, Laia

AU - Solà-Oriol, David

AU - Stein, Hans H.

AU - Su, Jian Qiang

AU - Brandt, Kristian K.

N1 - Publisher Copyright: © 2022 The Authors

PY - 2023

Y1 - 2023

N2 - Restrictions on antibiotic growth promoters have prompted livestock producers to use alternative growth promoters, and dietary copper (Cu) supplementation is currently being widely used in pig production. However, elevated doses of dietary Cu constitute a risk for co-selection of antibiotic resistance and the risk may depend on the type of Cu-based feed additives being used. We here report the first controlled experiment investigating the impact of two contrasting Cu-based feed additives on the overall swine gut microbiome and antibiotic resistome. DNA was extracted from fecal samples (n = 96) collected at four time points during 116 days from 120 pigs allotted to three dietary treatments: control, divalent copper sulfate (CuSO4; 250 μg Cu g−1 feed), and monovalent copper oxide (Cu2O; 250 μg Cu g−1 feed). Bacterial community composition, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) were assessed, and bioavailable Cu ([Cu]bio) was determined using whole-cell bacterial bioreporters. Cu supplementation to feed increased total Cu concentrations ([Cu]total) and [Cu]bio in feces 8–10 fold and at least 670–1000 fold, respectively, but with no significant differences between the two Cu sources. The swine gut microbiome harbored highly abundant and diverse ARGs and MGEs irrespective of the treatments throughout the experiment. Microbiomes differed significantly between pig growth stages and tended to converge over time, but only minor changes in the bacterial community composition and resistome could be linked to Cu supplementation. A significant correlation between bacterial community composition (i.e., bacterial taxa present) and ARG prevalence patterns were observed by Procrustes analysis. Overall, results of the experiment did not provide evidence for Cu-induced co-selection of ARGs or MGEs even at a Cu concentration level exceeding the maximal permitted level for pig diets in the EU (25 to 150 μg Cu g−1 feed depending on pig age).

AB - Restrictions on antibiotic growth promoters have prompted livestock producers to use alternative growth promoters, and dietary copper (Cu) supplementation is currently being widely used in pig production. However, elevated doses of dietary Cu constitute a risk for co-selection of antibiotic resistance and the risk may depend on the type of Cu-based feed additives being used. We here report the first controlled experiment investigating the impact of two contrasting Cu-based feed additives on the overall swine gut microbiome and antibiotic resistome. DNA was extracted from fecal samples (n = 96) collected at four time points during 116 days from 120 pigs allotted to three dietary treatments: control, divalent copper sulfate (CuSO4; 250 μg Cu g−1 feed), and monovalent copper oxide (Cu2O; 250 μg Cu g−1 feed). Bacterial community composition, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) were assessed, and bioavailable Cu ([Cu]bio) was determined using whole-cell bacterial bioreporters. Cu supplementation to feed increased total Cu concentrations ([Cu]total) and [Cu]bio in feces 8–10 fold and at least 670–1000 fold, respectively, but with no significant differences between the two Cu sources. The swine gut microbiome harbored highly abundant and diverse ARGs and MGEs irrespective of the treatments throughout the experiment. Microbiomes differed significantly between pig growth stages and tended to converge over time, but only minor changes in the bacterial community composition and resistome could be linked to Cu supplementation. A significant correlation between bacterial community composition (i.e., bacterial taxa present) and ARG prevalence patterns were observed by Procrustes analysis. Overall, results of the experiment did not provide evidence for Cu-induced co-selection of ARGs or MGEs even at a Cu concentration level exceeding the maximal permitted level for pig diets in the EU (25 to 150 μg Cu g−1 feed depending on pig age).

KW - Antibiotic resistance genes

KW - Antimicrobial resistance

KW - Bacterial community composition

KW - Co-selection

KW - HT-qPCR

KW - Pig microbiome

U2 - 10.1016/j.scitotenv.2022.159609

DO - 10.1016/j.scitotenv.2022.159609

M3 - Journal article

C2 - 36273560

AN - SCOPUS:85140464613

VL - 857

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

M1 - 159609

ER -

ID: 335686082