Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana. / Yu, Bo; Zheng, Wenna; Persson, Staffan; Zhao, Yang.

In: STAR Protocols, Vol. 4, No. 2, 102157, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yu, B, Zheng, W, Persson, S & Zhao, Y 2023, 'Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana', STAR Protocols, vol. 4, no. 2, 102157. https://doi.org/10.1016/j.xpro.2023.102157

APA

Yu, B., Zheng, W., Persson, S., & Zhao, Y. (2023). Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana. STAR Protocols, 4(2), [102157]. https://doi.org/10.1016/j.xpro.2023.102157

Vancouver

Yu B, Zheng W, Persson S, Zhao Y. Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana. STAR Protocols. 2023;4(2). 102157. https://doi.org/10.1016/j.xpro.2023.102157

Author

Yu, Bo ; Zheng, Wenna ; Persson, Staffan ; Zhao, Yang. / Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana. In: STAR Protocols. 2023 ; Vol. 4, No. 2.

Bibtex

@article{46033396310a4834bdc5067328f0cb14,
title = "Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana",
abstract = "Plant roots sense salt gradients in soil to avoid saline environments through halotropism. Here, we present a protocol to study halotropism with an optimized split-agar system that simulates the salt gradient in soil. We describe steps for preparation of the split-agar system, measurement of Na+, and observation of root bending. We then detail segmentation of root cells and visualization of microtubules and cellulose synthases. This system is simple to operate and has broader applications, such as hydrotropism and chemotropism. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).1",
keywords = "Cell Biology, Microscopy, Model Organisms, Molecular Biology, Plant Sciences",
author = "Bo Yu and Wenna Zheng and Staffan Persson and Yang Zhao",
note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
doi = "10.1016/j.xpro.2023.102157",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana

AU - Yu, Bo

AU - Zheng, Wenna

AU - Persson, Staffan

AU - Zhao, Yang

N1 - Publisher Copyright: © 2023 The Author(s)

PY - 2023

Y1 - 2023

N2 - Plant roots sense salt gradients in soil to avoid saline environments through halotropism. Here, we present a protocol to study halotropism with an optimized split-agar system that simulates the salt gradient in soil. We describe steps for preparation of the split-agar system, measurement of Na+, and observation of root bending. We then detail segmentation of root cells and visualization of microtubules and cellulose synthases. This system is simple to operate and has broader applications, such as hydrotropism and chemotropism. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).1

AB - Plant roots sense salt gradients in soil to avoid saline environments through halotropism. Here, we present a protocol to study halotropism with an optimized split-agar system that simulates the salt gradient in soil. We describe steps for preparation of the split-agar system, measurement of Na+, and observation of root bending. We then detail segmentation of root cells and visualization of microtubules and cellulose synthases. This system is simple to operate and has broader applications, such as hydrotropism and chemotropism. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).1

KW - Cell Biology

KW - Microscopy

KW - Model Organisms

KW - Molecular Biology

KW - Plant Sciences

U2 - 10.1016/j.xpro.2023.102157

DO - 10.1016/j.xpro.2023.102157

M3 - Journal article

C2 - 36917605

AN - SCOPUS:85149891966

VL - 4

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 102157

ER -

ID: 339679153