Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta): Advantage under Climate Change Scenarios?

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Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta) : Advantage under Climate Change Scenarios? / Permann, Charlotte; Herburger, Klaus; Felhofer, Martin; Gierlinger, Notburga; Lewis, Louise A.; Holzinger, Andreas.

In: Plants, Vol. 10, No. 8, 1740, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Permann, C, Herburger, K, Felhofer, M, Gierlinger, N, Lewis, LA & Holzinger, A 2021, 'Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta): Advantage under Climate Change Scenarios?', Plants, vol. 10, no. 8, 1740. https://doi.org/10.3390/plants10081740

APA

Permann, C., Herburger, K., Felhofer, M., Gierlinger, N., Lewis, L. A., & Holzinger, A. (2021). Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta): Advantage under Climate Change Scenarios? Plants, 10(8), [1740]. https://doi.org/10.3390/plants10081740

Vancouver

Permann C, Herburger K, Felhofer M, Gierlinger N, Lewis LA, Holzinger A. Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta): Advantage under Climate Change Scenarios? Plants. 2021;10(8). 1740. https://doi.org/10.3390/plants10081740

Author

Permann, Charlotte ; Herburger, Klaus ; Felhofer, Martin ; Gierlinger, Notburga ; Lewis, Louise A. ; Holzinger, Andreas. / Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta) : Advantage under Climate Change Scenarios?. In: Plants. 2021 ; Vol. 10, No. 8.

Bibtex

@article{ce4e3d245e7f427c8bac785f649418d6,
title = "Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta): Advantage under Climate Change Scenarios?",
abstract = "Extreme environments, such as alpine habitats at high elevation, are increasingly exposed to man-made climate change. Zygnematophyceae thriving in these regions possess a special means of sexual reproduction, termed conjugation, leading to the formation of resistant zygospores. A field sample of Spirogyra with numerous conjugating stages was isolated and characterized by molecular phylogeny. We successfully induced sexual reproduction under laboratory conditions by a transfer to artificial pond water and increasing the light intensity to 184 mu mol photons m(-2) s(-1). This, however was only possible in early spring, suggesting that the isolated cultures had an internal rhythm. The reproductive morphology was characterized by light- and transmission electron microscopy, and the latter allowed the detection of distinctly oriented microfibrils in the exo- and endospore, and an electron-dense mesospore. Glycan microarray profiling showed that Spirogyra cell walls are rich in major pectic and hemicellulosic polysaccharides, and immuno-fluorescence allowed the detection of arabinogalactan proteins (AGPs) and xyloglucan in the zygospore cell walls. Confocal RAMAN spectroscopy detected complex aromatic compounds, similar in their spectral signature to that of Lycopodium spores. These data support the idea that sexual reproduction in Zygnematophyceae, the sister lineage to land plants, might have played an important role in the process of terrestrialization.",
keywords = "alpine region, cell wall, conjugation, Spirogyra, sexual reproduction, streptophyte, zygospore, GREEN-ALGA ZYGNEMA, FRESH-WATER, SEXUAL REPRODUCTION, TERRESTRIAL ALGAE, ANTARCTIC STRAINS, NY-ALESUND, STREPTOPHYTA, DIVERSITY, EVOLUTION, PHOTOSYNTHESIS",
author = "Charlotte Permann and Klaus Herburger and Martin Felhofer and Notburga Gierlinger and Lewis, {Louise A.} and Andreas Holzinger",
year = "2021",
doi = "10.3390/plants10081740",
language = "English",
volume = "10",
journal = "Plants",
issn = "2223-7747",
publisher = "MDPI AG",
number = "8",

}

RIS

TY - JOUR

T1 - Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta)

T2 - Advantage under Climate Change Scenarios?

AU - Permann, Charlotte

AU - Herburger, Klaus

AU - Felhofer, Martin

AU - Gierlinger, Notburga

AU - Lewis, Louise A.

AU - Holzinger, Andreas

PY - 2021

Y1 - 2021

N2 - Extreme environments, such as alpine habitats at high elevation, are increasingly exposed to man-made climate change. Zygnematophyceae thriving in these regions possess a special means of sexual reproduction, termed conjugation, leading to the formation of resistant zygospores. A field sample of Spirogyra with numerous conjugating stages was isolated and characterized by molecular phylogeny. We successfully induced sexual reproduction under laboratory conditions by a transfer to artificial pond water and increasing the light intensity to 184 mu mol photons m(-2) s(-1). This, however was only possible in early spring, suggesting that the isolated cultures had an internal rhythm. The reproductive morphology was characterized by light- and transmission electron microscopy, and the latter allowed the detection of distinctly oriented microfibrils in the exo- and endospore, and an electron-dense mesospore. Glycan microarray profiling showed that Spirogyra cell walls are rich in major pectic and hemicellulosic polysaccharides, and immuno-fluorescence allowed the detection of arabinogalactan proteins (AGPs) and xyloglucan in the zygospore cell walls. Confocal RAMAN spectroscopy detected complex aromatic compounds, similar in their spectral signature to that of Lycopodium spores. These data support the idea that sexual reproduction in Zygnematophyceae, the sister lineage to land plants, might have played an important role in the process of terrestrialization.

AB - Extreme environments, such as alpine habitats at high elevation, are increasingly exposed to man-made climate change. Zygnematophyceae thriving in these regions possess a special means of sexual reproduction, termed conjugation, leading to the formation of resistant zygospores. A field sample of Spirogyra with numerous conjugating stages was isolated and characterized by molecular phylogeny. We successfully induced sexual reproduction under laboratory conditions by a transfer to artificial pond water and increasing the light intensity to 184 mu mol photons m(-2) s(-1). This, however was only possible in early spring, suggesting that the isolated cultures had an internal rhythm. The reproductive morphology was characterized by light- and transmission electron microscopy, and the latter allowed the detection of distinctly oriented microfibrils in the exo- and endospore, and an electron-dense mesospore. Glycan microarray profiling showed that Spirogyra cell walls are rich in major pectic and hemicellulosic polysaccharides, and immuno-fluorescence allowed the detection of arabinogalactan proteins (AGPs) and xyloglucan in the zygospore cell walls. Confocal RAMAN spectroscopy detected complex aromatic compounds, similar in their spectral signature to that of Lycopodium spores. These data support the idea that sexual reproduction in Zygnematophyceae, the sister lineage to land plants, might have played an important role in the process of terrestrialization.

KW - alpine region

KW - cell wall

KW - conjugation

KW - Spirogyra

KW - sexual reproduction

KW - streptophyte

KW - zygospore

KW - GREEN-ALGA ZYGNEMA

KW - FRESH-WATER

KW - SEXUAL REPRODUCTION

KW - TERRESTRIAL ALGAE

KW - ANTARCTIC STRAINS

KW - NY-ALESUND

KW - STREPTOPHYTA

KW - DIVERSITY

KW - EVOLUTION

KW - PHOTOSYNTHESIS

U2 - 10.3390/plants10081740

DO - 10.3390/plants10081740

M3 - Journal article

C2 - 34451785

VL - 10

JO - Plants

JF - Plants

SN - 2223-7747

IS - 8

M1 - 1740

ER -

ID: 279256587