Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies. / Ulvskov, Peter; Marcussen, Jan; Rajagopal, Ranganatha; Prinsen, Els; Rudelsheim, Patrick; Onckelen, Henri Van.

In: Plant and Cell Physiology, Vol. 28, No. 5, 07.1987, p. 937-945.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ulvskov, P, Marcussen, J, Rajagopal, R, Prinsen, E, Rudelsheim, P & Onckelen, HV 1987, 'Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies', Plant and Cell Physiology, vol. 28, no. 5, pp. 937-945. https://doi.org/10.1093/oxfordjournals.pcp.a077376

APA

Ulvskov, P., Marcussen, J., Rajagopal, R., Prinsen, E., Rudelsheim, P., & Onckelen, H. V. (1987). Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies. Plant and Cell Physiology, 28(5), 937-945. https://doi.org/10.1093/oxfordjournals.pcp.a077376

Vancouver

Ulvskov P, Marcussen J, Rajagopal R, Prinsen E, Rudelsheim P, Onckelen HV. Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies. Plant and Cell Physiology. 1987 Jul;28(5):937-945. https://doi.org/10.1093/oxfordjournals.pcp.a077376

Author

Ulvskov, Peter ; Marcussen, Jan ; Rajagopal, Ranganatha ; Prinsen, Els ; Rudelsheim, Patrick ; Onckelen, Henri Van. / Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies. In: Plant and Cell Physiology. 1987 ; Vol. 28, No. 5. pp. 937-945.

Bibtex

@article{d89b048f7dfc4a73a84d4ba9a835e322,
title = "Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies",
abstract = "A single-step immunoaffinity purification procedure using monoclonal antibodies was developed to isolate indole-3-acetamide from plant extracts. Antibodies from a selected clone, raised against IAA-Cl'-BSA, with pronounced ability to recognize indole-3-acetamide (IAM) were used to prepare an immunoaffinity absorbent. Antibodies purified by thiophilic interaction chromatography were immobilized on divinylsulfoneactivated agarose. This column shows a very high selectivity towards IAM compared to IAA. This single step of immunoaffinity purification gave plant extracts of sufficient purity for direct quantification by on-line spectrofluorimetry after an analytical ionsuppression-HPLC run. Successive approximation by a second analytical ion-pairing-HPLC run confirmed the validity of this analytical technique.",
keywords = "HPLC-on-line-fluorimetry, Immunoaffinity chromatography, Indole-3-acetamine, T-DNA, Thiophilic interaction chromatography, Tritiated indole-3-acetamide",
author = "Peter Ulvskov and Jan Marcussen and Ranganatha Rajagopal and Els Prinsen and Patrick Rudelsheim and Onckelen, {Henri Van}",
note = "Funding Information: Britta Nielsen is acknowledged for her skillful technical assistance. This work wassupported by the Danish Sugar Factories (Peter Ulvskov) and by the Danish Agricultural Research Council toJan Marcussen, vide J.nrs.: SJVF 13-3224 and 13-3406. Els Prinsen is recipient of an {"}Instituut voor Wetenschappelijk Onderzoek in Nijverheid en Landbouw{"} (IWONL) grant. Patrick Rudelsheim and Henri Van Onckelen are research assistant and senior research associate respectively of {"}Nationaal Fonds voor Wetenschappelijk Onderzoek{"} (NFWO).",
year = "1987",
month = jul,
doi = "10.1093/oxfordjournals.pcp.a077376",
language = "English",
volume = "28",
pages = "937--945",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - Immunoaffinity purification of indole-3-acetamide using monoclonal antibodies

AU - Ulvskov, Peter

AU - Marcussen, Jan

AU - Rajagopal, Ranganatha

AU - Prinsen, Els

AU - Rudelsheim, Patrick

AU - Onckelen, Henri Van

N1 - Funding Information: Britta Nielsen is acknowledged for her skillful technical assistance. This work wassupported by the Danish Sugar Factories (Peter Ulvskov) and by the Danish Agricultural Research Council toJan Marcussen, vide J.nrs.: SJVF 13-3224 and 13-3406. Els Prinsen is recipient of an "Instituut voor Wetenschappelijk Onderzoek in Nijverheid en Landbouw" (IWONL) grant. Patrick Rudelsheim and Henri Van Onckelen are research assistant and senior research associate respectively of "Nationaal Fonds voor Wetenschappelijk Onderzoek" (NFWO).

PY - 1987/7

Y1 - 1987/7

N2 - A single-step immunoaffinity purification procedure using monoclonal antibodies was developed to isolate indole-3-acetamide from plant extracts. Antibodies from a selected clone, raised against IAA-Cl'-BSA, with pronounced ability to recognize indole-3-acetamide (IAM) were used to prepare an immunoaffinity absorbent. Antibodies purified by thiophilic interaction chromatography were immobilized on divinylsulfoneactivated agarose. This column shows a very high selectivity towards IAM compared to IAA. This single step of immunoaffinity purification gave plant extracts of sufficient purity for direct quantification by on-line spectrofluorimetry after an analytical ionsuppression-HPLC run. Successive approximation by a second analytical ion-pairing-HPLC run confirmed the validity of this analytical technique.

AB - A single-step immunoaffinity purification procedure using monoclonal antibodies was developed to isolate indole-3-acetamide from plant extracts. Antibodies from a selected clone, raised against IAA-Cl'-BSA, with pronounced ability to recognize indole-3-acetamide (IAM) were used to prepare an immunoaffinity absorbent. Antibodies purified by thiophilic interaction chromatography were immobilized on divinylsulfoneactivated agarose. This column shows a very high selectivity towards IAM compared to IAA. This single step of immunoaffinity purification gave plant extracts of sufficient purity for direct quantification by on-line spectrofluorimetry after an analytical ionsuppression-HPLC run. Successive approximation by a second analytical ion-pairing-HPLC run confirmed the validity of this analytical technique.

KW - HPLC-on-line-fluorimetry

KW - Immunoaffinity chromatography

KW - Indole-3-acetamine

KW - T-DNA

KW - Thiophilic interaction chromatography

KW - Tritiated indole-3-acetamide

UR - http://www.scopus.com/inward/record.url?scp=70349864415&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.pcp.a077376

DO - 10.1093/oxfordjournals.pcp.a077376

M3 - Journal article

AN - SCOPUS:70349864415

VL - 28

SP - 937

EP - 945

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 5

ER -

ID: 308328443