Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.)

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.). / Hansen, Mads Anders Tengstedt; Ahl, Louise Isager; Pedersen, Henriette Lodberg; Westereng, Bjørge; Willats, William George Tycho; Jørgensen, Henning; Felby, Claus.

In: Industrial Crops and Products, Vol. 55, 2014, p. 63-69.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, MAT, Ahl, LI, Pedersen, HL, Westereng, B, Willats, WGT, Jørgensen, H & Felby, C 2014, 'Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.)', Industrial Crops and Products, vol. 55, pp. 63-69. https://doi.org/10.1016/j.indcrop.2014.02.002

APA

Hansen, M. A. T., Ahl, L. I., Pedersen, H. L., Westereng, B., Willats, W. G. T., Jørgensen, H., & Felby, C. (2014). Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.). Industrial Crops and Products, 55, 63-69. https://doi.org/10.1016/j.indcrop.2014.02.002

Vancouver

Hansen MAT, Ahl LI, Pedersen HL, Westereng B, Willats WGT, Jørgensen H et al. Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.). Industrial Crops and Products. 2014;55:63-69. https://doi.org/10.1016/j.indcrop.2014.02.002

Author

Hansen, Mads Anders Tengstedt ; Ahl, Louise Isager ; Pedersen, Henriette Lodberg ; Westereng, Bjørge ; Willats, William George Tycho ; Jørgensen, Henning ; Felby, Claus. / Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.). In: Industrial Crops and Products. 2014 ; Vol. 55. pp. 63-69.

Bibtex

@article{5f94448e4b2e4d198220c0b360c0964f,
title = "Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.)",
abstract = "Fuels and chemicals derived through biochemical conversion of agricultural by-products such as wheat straw (Triticum aestivum L.) is an area currently under intense research. In this study, separate leaves and stems were hydrothermally pretreated and enzymatically hydrolysed and analysed chemically and by comprehensive microarray polymer profiling (CoMPP). This way, the effects of each degradation step to the intermolecular organisation of specific polysaccharides in the cell walls were elucidated. After pretreatment, the degree of polymerisation (DP) of released xylo-oligosaccharides in both samples was up to about 20, but mostly around 3–8, and notably more acetylated in stems. Arabinoxylan (AX) and mixed-linkage glucan (MLG) became water-extractable while xylan, xyloglucan (XG), mannan and glucan remained only alkali-extractable. All polysaccharides became partly digestible after pretreatment however, regardless their extractability in water or only alkali. Based on the results, AX and MLG appear to be loosely bound in the cell wall matrix while the other polysaccharides are bound more tightly and shielded from enzymatic attack by AX and MLG until pretreatment. The gradual solubilisation and digestion of the polysaccharides during pretreatment and hydrolysis correlate well with previous models of the polysaccharides{\textquoteright} structural organisation in the cell wall.",
author = "Hansen, {Mads Anders Tengstedt} and Ahl, {Louise Isager} and Pedersen, {Henriette Lodberg} and Bj{\o}rge Westereng and Willats, {William George Tycho} and Henning J{\o}rgensen and Claus Felby",
year = "2014",
doi = "10.1016/j.indcrop.2014.02.002",
language = "English",
volume = "55",
pages = "63--69",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Extractability and digestibility of plant cell wall polysaccharides during hydrothermal and enzymatic degradation of wheat straw (Triticum aestivum L.)

AU - Hansen, Mads Anders Tengstedt

AU - Ahl, Louise Isager

AU - Pedersen, Henriette Lodberg

AU - Westereng, Bjørge

AU - Willats, William George Tycho

AU - Jørgensen, Henning

AU - Felby, Claus

PY - 2014

Y1 - 2014

N2 - Fuels and chemicals derived through biochemical conversion of agricultural by-products such as wheat straw (Triticum aestivum L.) is an area currently under intense research. In this study, separate leaves and stems were hydrothermally pretreated and enzymatically hydrolysed and analysed chemically and by comprehensive microarray polymer profiling (CoMPP). This way, the effects of each degradation step to the intermolecular organisation of specific polysaccharides in the cell walls were elucidated. After pretreatment, the degree of polymerisation (DP) of released xylo-oligosaccharides in both samples was up to about 20, but mostly around 3–8, and notably more acetylated in stems. Arabinoxylan (AX) and mixed-linkage glucan (MLG) became water-extractable while xylan, xyloglucan (XG), mannan and glucan remained only alkali-extractable. All polysaccharides became partly digestible after pretreatment however, regardless their extractability in water or only alkali. Based on the results, AX and MLG appear to be loosely bound in the cell wall matrix while the other polysaccharides are bound more tightly and shielded from enzymatic attack by AX and MLG until pretreatment. The gradual solubilisation and digestion of the polysaccharides during pretreatment and hydrolysis correlate well with previous models of the polysaccharides’ structural organisation in the cell wall.

AB - Fuels and chemicals derived through biochemical conversion of agricultural by-products such as wheat straw (Triticum aestivum L.) is an area currently under intense research. In this study, separate leaves and stems were hydrothermally pretreated and enzymatically hydrolysed and analysed chemically and by comprehensive microarray polymer profiling (CoMPP). This way, the effects of each degradation step to the intermolecular organisation of specific polysaccharides in the cell walls were elucidated. After pretreatment, the degree of polymerisation (DP) of released xylo-oligosaccharides in both samples was up to about 20, but mostly around 3–8, and notably more acetylated in stems. Arabinoxylan (AX) and mixed-linkage glucan (MLG) became water-extractable while xylan, xyloglucan (XG), mannan and glucan remained only alkali-extractable. All polysaccharides became partly digestible after pretreatment however, regardless their extractability in water or only alkali. Based on the results, AX and MLG appear to be loosely bound in the cell wall matrix while the other polysaccharides are bound more tightly and shielded from enzymatic attack by AX and MLG until pretreatment. The gradual solubilisation and digestion of the polysaccharides during pretreatment and hydrolysis correlate well with previous models of the polysaccharides’ structural organisation in the cell wall.

U2 - 10.1016/j.indcrop.2014.02.002

DO - 10.1016/j.indcrop.2014.02.002

M3 - Journal article

VL - 55

SP - 63

EP - 69

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -

ID: 112847681