Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation. / Zhong, Yingxin; Sagnelli, Domenico; Topbjerg, Henrik Bak; Hasler-Sheetal, Harald; Andrzejzcak, Olga; Hooshmand, Kourosh; Gislum, Rene; Jiang, Dong; Moller, Ian Max; Blennow, Andreas; Hebelstrup, Kim H.

In: Journal of Experimental Botany, Vol. 71, No. 1, 2020, p. 234-246.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhong, Y, Sagnelli, D, Topbjerg, HB, Hasler-Sheetal, H, Andrzejzcak, O, Hooshmand, K, Gislum, R, Jiang, D, Moller, IM, Blennow, A & Hebelstrup, KH 2020, 'Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation', Journal of Experimental Botany, vol. 71, no. 1, pp. 234-246. https://doi.org/10.1093/jxb/erz401

APA

Zhong, Y., Sagnelli, D., Topbjerg, H. B., Hasler-Sheetal, H., Andrzejzcak, O., Hooshmand, K., Gislum, R., Jiang, D., Moller, I. M., Blennow, A., & Hebelstrup, K. H. (2020). Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation. Journal of Experimental Botany, 71(1), 234-246. https://doi.org/10.1093/jxb/erz401

Vancouver

Zhong Y, Sagnelli D, Topbjerg HB, Hasler-Sheetal H, Andrzejzcak O, Hooshmand K et al. Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation. Journal of Experimental Botany. 2020;71(1):234-246. https://doi.org/10.1093/jxb/erz401

Author

Zhong, Yingxin ; Sagnelli, Domenico ; Topbjerg, Henrik Bak ; Hasler-Sheetal, Harald ; Andrzejzcak, Olga ; Hooshmand, Kourosh ; Gislum, Rene ; Jiang, Dong ; Moller, Ian Max ; Blennow, Andreas ; Hebelstrup, Kim H. / Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation. In: Journal of Experimental Botany. 2020 ; Vol. 71, No. 1. pp. 234-246.

Bibtex

@article{319e3783e60b46b688030bb616342033,
title = "Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation",
abstract = "The biosynthesis of starch granules in plant plastids is coordinated by the orchestrated action of transferases, hydrolases, and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interactions with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions, we expressed the protein module Carbohydrate-Binding Motif 20 (CBM20), which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and in formation of compound starch granules in grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology and number, and plant growth, as substantiated by a metabolic link between starch-protein interactions and control of CO2 fixation in chloroplasts.",
keywords = "Barley, Hordeum vulgare, photosynthesis, starch metabolism, starch-protein interactions, DOMAIN, METABOLISM, INITIATION, AMYLOPLAST, ENDOSPERM, PROTEINS, ENCODES, GROWTH",
author = "Yingxin Zhong and Domenico Sagnelli and Topbjerg, {Henrik Bak} and Harald Hasler-Sheetal and Olga Andrzejzcak and Kourosh Hooshmand and Rene Gislum and Dong Jiang and Moller, {Ian Max} and Andreas Blennow and Hebelstrup, {Kim H.}",
year = "2020",
doi = "10.1093/jxb/erz401",
language = "English",
volume = "71",
pages = "234--246",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation

AU - Zhong, Yingxin

AU - Sagnelli, Domenico

AU - Topbjerg, Henrik Bak

AU - Hasler-Sheetal, Harald

AU - Andrzejzcak, Olga

AU - Hooshmand, Kourosh

AU - Gislum, Rene

AU - Jiang, Dong

AU - Moller, Ian Max

AU - Blennow, Andreas

AU - Hebelstrup, Kim H.

PY - 2020

Y1 - 2020

N2 - The biosynthesis of starch granules in plant plastids is coordinated by the orchestrated action of transferases, hydrolases, and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interactions with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions, we expressed the protein module Carbohydrate-Binding Motif 20 (CBM20), which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and in formation of compound starch granules in grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology and number, and plant growth, as substantiated by a metabolic link between starch-protein interactions and control of CO2 fixation in chloroplasts.

AB - The biosynthesis of starch granules in plant plastids is coordinated by the orchestrated action of transferases, hydrolases, and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interactions with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions, we expressed the protein module Carbohydrate-Binding Motif 20 (CBM20), which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and in formation of compound starch granules in grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology and number, and plant growth, as substantiated by a metabolic link between starch-protein interactions and control of CO2 fixation in chloroplasts.

KW - Barley

KW - Hordeum vulgare

KW - photosynthesis

KW - starch metabolism

KW - starch-protein interactions

KW - DOMAIN

KW - METABOLISM

KW - INITIATION

KW - AMYLOPLAST

KW - ENDOSPERM

KW - PROTEINS

KW - ENCODES

KW - GROWTH

U2 - 10.1093/jxb/erz401

DO - 10.1093/jxb/erz401

M3 - Journal article

C2 - 31494665

VL - 71

SP - 234

EP - 246

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 1

ER -

ID: 249488128