Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]
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Isolation in vitro of Strongwellsea castrans [Fungi : Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]. / Eilenberg, J.; Wilding, N.; Bresciani, J.
In: Entomophaga, Vol. 37, No. 1, 01.03.1992, p. 65-77.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Isolation in vitro of Strongwellsea castrans [Fungi
T2 - Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]
AU - Eilenberg, J.
AU - Wilding, N.
AU - Bresciani, J.
PY - 1992/3/1
Y1 - 1992/3/1
N2 - The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.
AB - The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.
KW - Delia radicum
KW - entomogenous fungus
KW - Entomophthorales
KW - enzyme profile
KW - in vitro culture
KW - Strongwellsea castrans
UR - http://www.scopus.com/inward/record.url?scp=0003247816&partnerID=8YFLogxK
U2 - 10.1007/BF02372975
DO - 10.1007/BF02372975
M3 - Journal article
AN - SCOPUS:0003247816
VL - 37
SP - 65
EP - 77
JO - BioControl
JF - BioControl
SN - 1386-6141
IS - 1
ER -
ID: 200827769